Summary
Background: Over the last few years, estrogen receptor (ER) determination by immunohistochemistry (ER-ICA) has been extensively used, but it still remains to be established whether this method can replace the standard biochemical technique using dextran-coated charcoal (ERDCC).
Patients and methods: ER were determined by both the dextran-coated charcoal (DCC) method and immunohistochemistry (ICA) in 699 patients with primary breast cancer; other parameters (age, pathological T-pT- and nodal status -pN-, progesterone receptors by DCC, proliferative index by ICA) were also recorded. The ‘best’ cut-off for ERICA was evaluated by means of Receiver Operating Characteristics (R.O.C.) analysis; logistic regression analysis was used to find adequate ‘weights’ for stain intensity.
Results and conclusions: A significant correlation was found between the two methods (p < 0.001). R.O.C. analysis revealed that the ‘best’ cut-off for the ERICA score was 45% (sensitivity 0.810, specificity 0.804). Logistic regression analysis showed that an ERICA score which also considers staining intensity does not add any useful information concerning ER content in breast cancers.
References
Wollenweider-Zerargui L, Barrelet L, Wong Y, Lemarchand-Beraud I, Gomez F: The predictive value of estrogen and progesterone receptor concentrations on the clinical behavior of breast cancer in women. Clinical correlations in 547 patients. Cancer 57: 1171–1180, 1988
Williams MR, Todd JH, Ellis IO, Dowle CS, Haybittle JL, Elston CW, Nicholson RI, Griffiths K, Blamey RW: Oestrogen receptors in primary and advanced breast cancer. An eight-year review of 704 cases. Br J Cancer 55: 67–73, 1987
Alexieva-Figusch J, van Putten WL, Blankestein M: The prognostic value and relationships of patient characteristics, estrogen and progestin receptors, and site of relapse in primary breast cancer. Cancer 61: 758–768, 1988
Clark GM, McGuire WL: Steroid receptors and other prognostic factors in primary breast cancer. Semin Oncol 15 (supp.): 20–25, 1988
Molino A, Turazza M, Bonetti A, Biondani P, Griso C, Adami L, Corgnati A, Nortilli R, Pollini GP, Modena S, Cetto GL: Estrogen and progesterone receptors in breast cancer: correlation with clinical and pathological features and with prognosis. Oncology 49: 82–88, 1992
Early breast cancer trialists collaborative group: Systemic treatment of early breast cancer by hormonal, cytotoxic or immune therapy. Lancet 339: 1–15, 71-85, 1992
Pertschuk LP, Eisenberg KB, Carter AC, Feldman J: Immunohistologic localization of estrogen receptors in breast cancer with monoclonal antibodies. Cancer 55: 1513–1518, 1985
McCarty KS Jr, Miller LS, Cox EB, Konrath J, McCarty KS Sr: Estrogen receptor analysis. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies. Arch Path Lab Med 109: 716–721, 1985
Pascal RR, Santeusano G, Sarrel D, Johnson CE: Immunohistologic detection of estrogen receptors in paraffin-embedded breast cancers: correlation with cytosol measurements. Hum Path 17: 370–375, 1986
Reiner A, Reiner G, Spona J, Teleky B, Holzner JH: Estrogen receptor analysis and biopsies and fine-needle aspirates from human breast carcinoma: correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies. Am J Pathol 125: 443–449, 1986
Bruun Rasmussen B, Thorpe SM, Norgaard T, Rasmussen J, Agdal N, Rose C: Immunohistochemical steroid receptor detection in frozen breast cancer tissue. A multicenter investigation. Acta Oncologica 27 (6A): 757–760, 1988
De Lena M, Marzullo F, Simone G, Labriola A, Tommasi S, Petroni S, Paradiso A: Correlation between ERICA and DCC assay in hormone receptor assessment of human breast cancer. Oncology 45: 308–312, 1988
Helin HJ, Helle MJ, Helin ML, Isola JJ: Immunocytochemical detection of estrogen and progesterone receptors in 124 human breast cancers. Am J Clin Pathol 89: 137–142, 1988
Scheres HME, de Goeij AFPM, Rousch MJM, Hondius GG, Willebrand DD, Gijzen AH, Bosman FT: Quantification of oestrogen receptors in breast cancer: radiochemical assay on cytosols and cryostat sections compared with semiquantitative immunocytochemical analysis. J Clin Pathol 41: 623–632, 1988
Parl FF, Posey YF: Discrepancies of biochemical and immunohistochemical estrogen receptor assays in breast cancer. Human Pathology 19 (8): 960–966, 1988
Wong SY, Path MRC, Carrol EDS, Ah-See SY, Sewell HF: Detection of estrogen receptor proteins in breast tumors using an improved APAAP immunohistochemical technique. Cancer 62: 2171–2175, 1988
Wedad H, Mobbs BG: Comparative evaluation of ER-ICA and enzyme immunoassay for the quantitation of estrogen receptors in breast cancer. Am J Clin Pathol 91: 182–186, 1989
Patterson DA, Reid CP, Anderson TJ, Hawkins RA: Assessment of oestrogen receptor content of breast carcinoma by immunohistochemical techniques on fixed and frozen tissue and by biochemical ligand binding assay. J Clin Pathol 43: 46–51, 1990
Sondergaard G, Pedersen KO, Paulsen SM: Estrogen receptor analyses in breast cancer: comparison of monoclonal immunohistochemical and biochemical methods. Eur J Cancer Clin Oncol 25: 1425–1429, 1989
Molino A, Bonetti F, Bonetti A, Turazza M, Capelli P, Piazzola E, Martignoni G, Pollini G, Modena S, Nortilli R, Pradella R, Corgnati A, Piubello Q, Cetto GL: Prognostic significance of estrogen receptors, determined by two different methods in breast cancer (abstract). Ann Oncol 1: 8.16, 1990
Pertschuk LP, Kim DS, Nayer K, Feldman JG, Eisenberg KB, Carter AC, Rong ZT, Thelmo WL, Fleisher J, Greene GL: Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Cancer 66: 1663–1670, 1990
Katz RL, Patel S, Sneige N, Fritsche HA, Hortobagyi GN, Ames FC, Brooks T, Ordonez NG: Comparison of immunocytochemical and biochemical assays for estrogen receptor in fine needle aspirates and histologic sections from breast carcinomas. Breast Cancer Res Treat 15: 191–203, 1990
Allred DC, Bustamante MA, Daniel CO, Gaskill HV, Cruz AB: Immunocytochemical analysis of estrogen receptors in human breast carcinomas. Arch Surg 125: 107–113, 1990
U.I.C.C. International Union Against Cancer: TNM Atlas. Illustrated guide to the TNM/pTNM classification of malignant tumours. Springer Verlag, Berlin, Heidelberg, 1992
EORTC breast cancer co-operative group: Revision of standards for the assessment of hormone receptors in human breast cancer: report of the second EORTC workshop, held on March 16–17, 1979, in the Netherlands Cancer Institute. Eur J Cancer 16: 1513–1515, 1980
Gerdes J, Pickartz H, Brotherton J, Hammerstein J, Weitzel H, Stein H: Growth fractions and estrogen receptors in human breast cancers as determinedin situ with monoclonal antibodies. Am J Pathol 129: 486–492, 1987
Metz CE: Basic principles of R.O.C. analysis. Semin Nucl Med 8: 238- 298, 1978
McNeil BJ, Adelstein SP: Determining the value of diagnostic and screening tests. J Nucl Med 17: 439–448, 1976
Weinstein J, Finenberg R: Clinical Decision Analysis, WR Saunders Co., Philadelphia, 114–127, 1980
Cox DR: The analysis of binary data. Chapman and Hall London, 1970
Payne CD: The Glim System Release 3.77 Manual. Royal Statistical Society London, 1986
Cudahy TJ, Boeryd BR, Franlund BK, Nordenskjold BA: A comparison of three different methods for the determination of estrogen receptors in human breast cancer. Am J Clin Pathol 90: 583–590, 1988
Fisher B, Redmond C, and others for the National Surgical Adjuvant Breast Project: Systemic therapy in node-negative patients: updated NSABP clinical trials. J Natl Cancer Inst Monogr 11: 105–116, 1992
Zambetti M, Bonadonna G, Valagussa P, Daidone MG, Coradini D, Bignami P, Contesso G, Silvestrini R: Adjuvant CMF for node-negative and estrogen receptor-negative breast cancer patients. J Natl Cancer Inst Monogr 11: 77–83, 1992
Tosi P, Baak JPA, Luzi P, Sforza V, Santopietro R, Lio R: Correlation between immunohistochemically determined oestrogen receptor content, using monoclonal antibodies and qualitative and quantitative tissue features in ductal breast cancer. Histopathology 11: 741–751, 1987
Gaskell DJ, Sangster K, Tesdale AL, Carson D, Hawkins RA: Change in the oestrogen receptor status of breast cancer with age-comparison of two types of assay. Br J Cancer 66: 610–613, 1992
Fisher B, Wickerman DL, Brown A: Breast cancer estrogen and progesterone receptor values: their distribution, degree of concordance, and relation to number of positive axillary nodes. J Clin Oncol 1: 349–358, 1983
Thorpe SM, Carsten R: Oestrogen and progesterone receptor determinations in breast cancer. Technology and Biology Cancer Surv 5: 505–525, 1986
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Molino, A., Micciolo, R., Turazza, M. et al. Estrogen receptors in 699 primary breast cancers: A comparison of immunohistochemical and biochemical methods. Breast Cancer Res Tr 34, 221–228 (1995). https://doi.org/10.1007/BF00689713
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DOI: https://doi.org/10.1007/BF00689713