Summary
Using the series system cell receptor→mistletoe lectin → antiferritin-antibody→ferritin, the possibilities for combination of lectin and immunological reactions for histochemistry are discussed.
The system cell antigen→ antibody → labelled mistletoe (or other) lectin is recommended for visualization of cell antigens (mistletoe lectin as common immunoglobulin reagent). It is pointed out that lectin reactions do not belong to immunhistochemistry but to affinity histochemistry. For all receptor specific proteins (antibodies, lectins, enzymes, haptoglobin and other) the term affinitin is proposed. In consideration of this new definition a common scheme is formulated: Affinitin reacts with affinitin receptor forming affinity product.
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References
Asperg, K., Porath, I.: Group-specific adsorption of glycoproteins. Acta Chem. Scand.24, 1839–1841 (1970)
Bernhard, W., Avrameas, S.: Ultrastructural visualization of cellular carbohydrate components by means of Concanavalin A Exp. Cell Res.64, 232–236 (1971)
Ersson, B., Porath, I.: Immobilized lectins as adsorbents for serum proteins. FEBS Lett.48, 126–129 (1974)
Franz, H., Haustein, B., Luther, P., Kindt, A.: Isolierung und Charakterisierung von Inhaltsstoffen der Mistel (Viscum album L). I. Affinitätschromatographie von Mistelrohextrakten an fixierten Plasmaproteinen Acta Biol. Med. Germ.36, 113–117 (1977)
Franz, H., Ziska, P., Roth, J.: Heavy metal carbohydrate complexes for demonstration of lectin surface receptors. The preparation of a galactan-iron complex. Acta Histochem. (Jena)57, 165–166 (1976)
Franz, H., Roth, J.: Preparation of carbohydrate-iron complexes, as electron-dense receptors for membrane bound lectins. Histochemistry41, 361–363 (1975)
Hjelm, H., Hjelm, K., Sjöquist, J.: Protein A from staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett.28, 73–76 (1972)
Kharilonenko, I.G.: Der Einsatz der Affinitätschromatographie in der Virologie. Voprosi Virusol.1, 97–104 (1977)
Martin, B., Spicer, S.S.: Concanavalin A — iron dextran technique for staining cell surface mucosub-strances. J. Histochem. Cytochem.22, 206–207 (1974)
Nicolson, G.L., Singer, S.J.: Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes. Proc. Natl. Acad. Sci. USA68, 942–945 (1974)
Owen, P., Oppenheim, I.O., Nachbar, M.S., Kessler, R.E.: The use of lectins in the quantitation and analysis of macromolecules by affinity-electrophoresis. Analyt. Biochem.80, 446–457 (1977)
Shinjyo, S., ABE, H., Masuda, M.: Carbohydrate composition of horse spleen ferritin. Biochim. Biophys. Acta411, 165–167 (1975)
Singer, S.J., Schick, A.F.: The properties of specific stains for electron microscopy prepared by the conjugation of antibody molecules with ferritin. J. Biophys. Biochem. Cytol.9, 519–537 (1961)
Weinstein, Y., Givol, D., Strausbach, P.H.: The Fractionation of Immunoglobulins with Insolubilized Concanavalin A. J. Immunol.109, 1402–1404 (1972)
Ziska, P., Franz, H., Kindt, A.: The lectin from Viscum album L. purification by biospecific affinity chromatography. Experientia34, 123 (1978)
Ziska, P., Mohr, J.: Affinity chromatography of serum proteins using immobilized lectin from Vicia faba Acta Biol. Med. Germ.36, 1197–1198 (1977)
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Franz, H., Bergmann, P. & Ziska, P. Combination of immunological and lectin reactions in affinity histochemistry: Proposition of the term affinitin. Histochemistry 59, 335–342 (1979). https://doi.org/10.1007/BF00689615
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DOI: https://doi.org/10.1007/BF00689615