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Histochemistry

, Volume 59, Issue 4, pp 259–269 | Cite as

Histochemical demonstration of a circadian rhythm of succinate dehydrogenase in rat pineal gland. Influence of coenzyme Q10 addition

  • M. Møller
  • P. E. Høyer
Article

Summary

Succinate dehydrogenase activity was investigated histochemically in the rat pineal gland. The influence of fixation on the activity pattern, the possible diffusion of enzyme, the nothing dehydrogenase reaction, and the substantivity of the tetrazolium salts and formazans were investigated in control experiments.

In rats maintained on a 17/7 h light/dark schedule a distinct circadian rhythm of the succinate dehydrogenase was demonstrated in the pineal gland. Activity was lowest during the day and highest during the night. The dorsocaudal part of the gland showed the highest activity and within the same part of the gland the activity varied between individual pinealocytes. A relative lack of endogenous coenzyme Q, as well as a circadian rhythm of this coenzyme, highly influenced the activity of succinate dehydrogenase. It is concluded that succinate dehydrogenase activity in the pineal gland of the rat is regulated by changing the concentration of the active enzyme itself as well as the level of the endogenous coenzyme Q. Whether this is caused by a circadian rhythm in the synthesis or in the catabolism of the enzyme and the coenzyme was not revealed by the present study.

Keywords

Public Health Active Enzyme High Activity Succinate Control Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Andersen, H., Høyer, P.E.: Studies in succinate dehydrogenase histochemistry. Histochemie35, 173–188 (1973)Google Scholar
  2. Andersen, H., Høyer, P.E.: Simplified control experiments in the histochemical study of coenzymelinked dehydrogenases. Histochemistry38, 71–83 (1974)Google Scholar
  3. Andersen, H., Contestabile, A.: The pyridine nucleotide and non-pyridine nucleotide dependence of L-glutamate dehydrogenase in the histochemical system. Histochemistry53, 117–133 (1977)Google Scholar
  4. Bayerová, G., Bayer, A.: Beitrag zur Fermenthistochemie der menschlichen Epiphyse. Acta Histochem (Jena)28, 169–173 (1967)Google Scholar
  5. Bäckström, M., Wetterberg, L.: Increased N-acetylserotonin and melatonin induced by d-amphetamine in rat pineal gland organ culture via a beta-adrenergic receptor mechanism. Acta Physiol. Scand.87, 113–120 (1973)Google Scholar
  6. Bostelmann, W.: Das ultrastrukturelle und enzymhistochemische Verhalten der Rattenzirbeldrüse nach Funktionsphasenwechsel durch Dauerbeleuchtung und ständige Dunkelheit. Endokrinologie53, 365–384 (1968)Google Scholar
  7. Bostelmann, W.: Enzymhistochemische und submikroskopische Untersuchungen zur Reperation der Rattenzirbeldrüse nach experimenteller Parenchymnekrose. Acta Neuropathologica11, 140–156 (1968)Google Scholar
  8. Bostelmann, W., Bienengräber, A.: Enzymhistochemie der Epiphysis cerebri des Kaninchens. Acta Neuropathologica2, 461–469 (1963)Google Scholar
  9. Botticelli, A., Trentini, G.P., Barbolini, G., De Gaetani, C.F.: Histoenzymology of the “circumventricular organs” of the rat. V. The oxidoreductases, the transferases and the hydrolases of the pineal gland. Acta Histochem. (Jena)44, 288–299 (1972)Google Scholar
  10. Conn, E.E., Stumpf, P.K.: Outlines of Biochemistry. New York-London-Sydney-Toronto: John Wiley & Sons 1976Google Scholar
  11. Contestabile, A., Andersen, H.: Methodological aspects of the histochemical localization and activity of some cerebellar dehydrogenases. Histochemistry56, 117–132 (1978)Google Scholar
  12. Freund, D., Arendt, J., Vollrath, L.: Tentative immunohistochemical demonstration of melatonin in the rat pineal gland. Cell Tissue Res.181, 239–244 (1977)Google Scholar
  13. Garbarsch, C., Andersen, H., Høyer, P.E.: Succinate dehydrogenase activity in the wall of rabbit aorta. The histochemical use of PMS and exogenous coenzyme Q10 as intermediate carriers. Histochemistry57, 297–304 (1978)Google Scholar
  14. Høyer, P.E., Andersen, H.: Histochemistry of 3β-hydroxysteroid dehydrogenase in rat ovary. I. A methodological study. Histochemistry51, 167–193 (1977)Google Scholar
  15. Høyer, P.E., Møller, M.: Histochemistry of 11β-hydroxysteroid dehydrogenase in rat submandibular gland. Effect of cortisol stimulation. Histochem. J.9, 599–618 (1977)Google Scholar
  16. Kappers, J.A.: The development, topographical relations and innervation of the epiphysis cerebri in the albino rat. Z. Zellforsch.52, 163–215 (1960)Google Scholar
  17. Klein, D.C., Weller, J.L., Moore, R.Y.: Melatonin metabolism: neural regulation of pineal serotonin: acetyl coenzyme A N-acetyltransferase activity. Proc. Natl. Acad. Sci. USA68, 3107–3110 (1971)Google Scholar
  18. Klein, D.C., Yuwiler, A., Weller, J.L., Plotkin, S.: Postsynaptic adrenergic-cyclic AMP control of the serotonin content of cultured rat pineal glands. J. Neurochem.21, 1261–1271 (1973)Google Scholar
  19. Krass, M.E., LaBella, F.S., Shin, S.H., Minnich, J.: Biochemical features of the pineal compared with other endocrine and nervous tissues. Mem. Soc. Endo.19, 49–76 (1971)Google Scholar
  20. Le Gros Clark, W.E.: The nervous and vascular relations of the pineal gland. J. Anat. (Cambridge)74, 471–494 (1940)Google Scholar
  21. Lehninger, A.L.: Biochemistry. New York: Worth Publishers 1975Google Scholar
  22. Lojda, Z., Gossrau, R., Schiebler, T.H.: Enzym-histochemische Methoden. Berlin-Heidelberg-New York: Springer-Verlag 1976Google Scholar
  23. Mahler, H.R., Cordes, E.H.: Biological Chemistry. New York-Evanston-San Francisco-London: Harper & Row 1971Google Scholar
  24. Montgomery R., Dryer, R.L., Conway, T.W., Spector, A.A.: Biochemistry. A case-oriented Approach. Saint Louis: Mosby 1977Google Scholar
  25. Moore, R.Y.: Retinohypothalamic projection in mammals: A comparative study. Brain Res.49, 403–409 (1973)Google Scholar
  26. Moore, R.Y., Heller, A., Bhatnager, R.K., Wurtman, R.J., Axelrod, J.: Central control of the pineal gland: visual pathways. Arch. Neurol.18, 208–218 (1968)Google Scholar
  27. Moore, R.Y., Klein, D.C.: Visual pathways and the central neural control of a circadian rhythm in pineal serotonin N-acetyltransferase activity. Brain Res.71, 17–33 (1974)Google Scholar
  28. Pearse, A.G.E.: Histochemistry, Theoretical and Applied. Edinburgh-London: Churchill Livingstone 1972Google Scholar
  29. Quay, W.B.: Experimental modifications and changes with age in pineal succinic dehydrogenase activity. J. Physiol.196, 951–955 (1958)Google Scholar
  30. Quay, W.B.: Pineal chemistry in cellular and physiological mechanisms. Springfield, Illinois: Charles C. Thomas (1974)Google Scholar
  31. Reiter, R.J.: Endocrine rhythms associated with pineal gland function. Adv. Exp. Med. Biol.54, 43–73 (1975)Google Scholar
  32. Rudeen, P.K., Reiter, R.J., Vaughan, M.K.: Pineal serotonin-acetyl-transferase activity in four mammalian species. Neurosci. Letters1, 225–229 (1975)Google Scholar
  33. Snedecor, G.W., Cochran, W.G.: Statistical Methods. Ames-Iowa: Iowa State University Press (1967)Google Scholar
  34. Tapp, E., Huxley, M., Davies, S.: The enzyme histochemistry of the pineal gland in rats. Histochem. J.5, 49–56 (1973)Google Scholar
  35. Vollrath, L., Schmidt, D.S.: Enzymhistochemische Untersuchungen an der Zirbeldrüse normaler und trächtiger Meerschweinchen. Histochemie20, 328–337 (1969)Google Scholar
  36. Wattenberg, L.W., Leong, J.L.: Effects of coenzyme Q10 on succinic dehydrogenase activity as measured by tetrazolium salt reductions. J. Histochem. Cytochem.8, 296–303 (1960)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • M. Møller
    • 1
    • 2
  • P. E. Høyer
    • 1
    • 2
  1. 1.Anatomy Department BUniversity of CopenhagenCopenhagenDenmark
  2. 2.Laboratory of Histochemistry and Cytochemistry, Anatomy Department AUniversity of CopenhagenCopenhagenDenmark

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