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Archives of Microbiology

, Volume 117, Issue 1, pp 53–60 | Cite as

Regulatory properties of the nitrogenase fromRhodopseudomonas palustris

  • W. G. Zumft
  • F. Castillo
Article

Abstract

Ammonium salts, glutamine, asparagine, and urea cause an immediate inactivation (switch-off) of light-dependent acetylene reduction in intact cells of the photosynthetic bacteriumRhodopseudomonas palustris. This effect is reversible showing the same kinetic pattern of inactivation and reactivation with all effector compounds. Its duration depends on the amount of effector added to the cells. Both nitrogenase components are found catalytically active in a cell-free preparation after enzyme switch-off in vivo. Involvement of the ammonia assimilating system in this regulatory mechanism is indicated by the following observations: ammonia uptake during the switch-off period, resumption of acetylene reduction after disappearance of ammonia from the outer medium, and persistence of enzyme switch-off with dihydrogen and thiosulfate as electron donors in the absence of an additional carbon source. Nitrogenase activity in crude extracts is non-linear with time and is stimulated by manganese ions. After resolution of nitrogenase into its MoFe-protein and Fe-protein these properties are lost, indicating the presence of an activating factor. Nitrogenase ofR. palustris cross reacts reciprocally with the complementary proteins ofAzotobacter vinelandii, but not with those ofClostridium pasteurianum.

Key words

Rhodopseudomonas palustris Nitrogenase Regulation Ammonia Cross reactivity 

Abbreviations

CCCP

m-chlorocarbonyl cyanide phenyl hydrazone

DNP

2,4-dinitrophenol

EPR

electron paramagnetic resonance

HEPES

N-2-hydroxyethylpiperazine-Ń-2-ethane sulfonic acid

NOQNO

2-n-nonyl-4-hydroxyquinoline-N-oxide

TES

N-tris[hydroxymethyl]methyl-2-aminoethane sulfonic acid

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • W. G. Zumft
    • 1
  • F. Castillo
    • 1
  1. 1.Institut für Botanik der UniversitätErlangenGermany

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