Changes in the level of octopine during the escape responses of the scallop,Pecten maximus (L.)
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The activities of octopine dehydrogenase (ODH) and α-glycerophosphate dehydrogenase (GDH) were determined in several tissues ofPecten maximus. The ratios of ODH/GDH were 13 for the adductor muscle, 9 for the mantle and 3 for the digestive gland and the gill. Therefore, the main pathway for the glycolytic re-oxidation of NADH seemed to be via the formation of octopine.
During burst activity the level of arginine phosphate in the adductor muscle declined concurently with an increase in arginine. Little octopine, however, accumulated until the animals were exhausted; the main increase in octopine concentration took place during the beginning of the subsequent rest period.
It is concluded that inPecten maximus there is no “flare-up” of glycolysis during burst activity. Instead the increased demand for ATP is met by the breakdown of arginine phosphate. Octopine accumulates later, possibly due to a stimulation of glycolysis brought about by the activation of phosphofructokinase by AMP at low levels of arginine phosphate.
KeywordsPhosphate Adductor Muscle Arginine NADH Human Physiology
α-glycerophosphate dehydrogenase (E.C.22.214.171.124)
octopine dehydrogenase (E.C.126.96.36.199)
lactate dehydrogenase (E.C.188.8.131.52)
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