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Journal of comparative physiology

, Volume 124, Issue 2, pp 121–127 | Cite as

Changes in the level of octopine during the escape responses of the scallop,Pecten maximus (L.)

  • Gerd Gäde
  • Ernst Weeda
  • Peter A. Gabbott
Article

Summary

  1. 1.

    The activities of octopine dehydrogenase (ODH) and α-glycerophosphate dehydrogenase (GDH) were determined in several tissues ofPecten maximus. The ratios of ODH/GDH were 13 for the adductor muscle, 9 for the mantle and 3 for the digestive gland and the gill. Therefore, the main pathway for the glycolytic re-oxidation of NADH seemed to be via the formation of octopine.

     
  2. 2.

    During burst activity the level of arginine phosphate in the adductor muscle declined concurently with an increase in arginine. Little octopine, however, accumulated until the animals were exhausted; the main increase in octopine concentration took place during the beginning of the subsequent rest period.

     
  3. 3.

    It is concluded that inPecten maximus there is no “flare-up” of glycolysis during burst activity. Instead the increased demand for ATP is met by the breakdown of arginine phosphate. Octopine accumulates later, possibly due to a stimulation of glycolysis brought about by the activation of phosphofructokinase by AMP at low levels of arginine phosphate.

     

Keywords

Phosphate Adductor Muscle Arginine NADH Human Physiology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

GDH

α-glycerophosphate dehydrogenase (E.C.1.1.1.8)

ODH

octopine dehydrogenase (E.C.1.5.1.11)

LDH

lactate dehydrogenase (E.C.1.1.1.27)

DTT

dithiothreitol

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Gerd Gäde
    • 1
  • Ernst Weeda
    • 1
  • Peter A. Gabbott
    • 2
  1. 1.Institut für ZoophysiologieUniversität Bonn, AVZ IBonnFederal Republic of Germany
  2. 2.Marine Science LaboratoriesNERC Unit of Marine Invertebrate BiologyAngleseyUK

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