Summary
A new tumour model that is particularly suitable for testing intraperitoneal chemotherapy is described. Single tumours were induced to grow in the mesentery of rats by the implantation of small pieces taken from subcutaneous tumours. Tumour growth was monitored by repeated laparotomies at which the tumour size was measured with calipers. In this way, growth curves of treated and untreated tumours could be defined. The diameter of untreated intraperitoneal tumours increased linearly with time [diameter (mm)=0.39 t (days) +2.4]. Tests using different numbers of laparotomies showed that the procedure itself had little influence on growth. Cell kinetic studies of 6-mm tumours showed a mean labelling index of 31% and a volume-doubling time of 3.9 days, resulting in cell-loss factors probably in excess of 70%. The model was tested by assessing the effect of the chemotherapeutic agent cisplatin. Regression and regrowth could be satisfactorily followed, leading to estimates of growth delay. This model therefor provides a quantitative way to assess the response of intraperitoneal tumours to chemotherapy.
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References
Begg AC (1987) Principles and practice of the tumor growth delay assay. In: Kallman RF (ed) Rodent tumor models in experimental cancer therapy. Pergamon Press, Oxford, pp 114–121
Begg AC, Moonen L, Hofland I, Dessing M, Bartelink H (1988) Human tumour cell kinetics using a monoclonal antibody against iododeoxyuridine: intratumoral sampling variations. Radiother Oncol 11: 337–347
Brenner DE (1986) Intraperitoneal chemotherapy: a review. J Clin Oncol 4: 1135–1147
Denekamp J (1970) The cell proliferation kinetics of animal tumors. Cancer Res 30: 393–400
Los G, Ruevekamp M, Bosnie N, De Graaf PW, McVie JG (1990) Intraperitoneal tumour growth and chemotherapy in a rat model. Eur J Cancer Clin Oncol 25: 1057–1066
Hamilton TC, Young RC, Louie KG, Behrense BC, McKoy WM, Grotzinger KR, Ozols RF (1984) Characteristics of a xenograft model of human ovarian carcinoma which produces ascites and intraabdominal carcinomatosis in mice. Cancer Res 44: 3286–3289
Marquet RL, Westbrook DL, Jeekel J (1984) Interferon treatment of a transplantable rat colon adenocarcinoma: importance of tumor site. Int J Cancer 33: 689–692
Steel GG (1977) Growth kinetics of tumours. Oxford University Press, Oxford
Ten Bokkel Huinink WW, Dubbelman R, Aartsen A, Franklin H, McVie JG (1985) Experimental and clinical results with intraperitoneal cisplatin. Semin Oncol 12 [Suppl 4]: 43–46
Thomlinson RH, Craddock EA (1967) The gross response of an experimental tumour to single doses of X-rays. Br J Cancer 21: 108–123
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Nagel, J.D., Los, G., Begg, A.C. et al. A new intraperitoneal tumor model in the rat. Cancer Chemother. Pharmacol. 27, 121–124 (1990). https://doi.org/10.1007/BF00689095
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DOI: https://doi.org/10.1007/BF00689095