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Acta Neuropathologica

, Volume 23, Issue 1, pp 43–47 | Cite as

Uptake and retrograde axonal transport of protein tracers in hypoglossal neurons

Fate of the tracer and reaction of the nerve cell bodies
  • Krister Kristensson
  • Yngve Olsson
Original Investigations

Summary

Protein tracers (albumin labelled with Evan's blue and horseradish peroxidase) accumulate in the cytoplasm of hypoglossal neurons in the brain stem following injection into the tongue of rats and mice resulting apparently from axonal uptake and subsequent retrograde transport from the periphery. The present study was performed to follow the fate of the protein tracers and to find out if they induce any signs of neuronal necrosis.

The proteins remained in the nerve cell bodies for about 6–11 days as revealed by fluorescence microscopy and light microscopical enzyme histochemistry after the injection into the tongue. Thereafter the proteins disappeared from the neurons presumably as a result of lysosomal degradation. The incorporation of foreign proteins did not produce as observed by light and electron microscopy any signs of cell degeneration.

Key words

Retrograde Axonal Transport Neuronal Degradation Foreign Proteins 

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References

  1. Brightman, M. W., Klatzo, I., Olsson, Y., Reese, T. S.: The blood-brain barrier to proteins under normal and pathological conditions. J. Neurol. Sci.10, 215–239 (1970).Google Scholar
  2. Brownstone, Y. S., Chapman-Andresen, C.: Degradation of endocytosed albumin by Chaos chaos. C. R. Carlsberg38, 297–313 (1971).Google Scholar
  3. Dales, S., Silverberg, H.: Viropexis of herpes simplex virus by HeLa cells. Virology37, 475–480 (1969).Google Scholar
  4. Ehrenreich, B. A., Cohn, Z. A.: The uptake and digestion of iodinated human serum albumin by macrophages in vitro. J. exp. Med.126, 941–958 (1967).Google Scholar
  5. Friede, R. L.: Topographic brain chemistry. New York-London: Academic Press 1966.Google Scholar
  6. Gabathuler, M.-P., Ryser, H. J.-P.: The digestive function of lysosomes as studied by the turnover of ingested foreign macromolecules. Proc. roy. Soc. B173, 95–98 (1969).Google Scholar
  7. Graham, R. C., Karnovsky, M. J.: The early stages of absorbtion of injected horseradish peroxidase in the proximal tubules of mouse kidney; Ultrastructural correlates by a new technique. J. Histochem. Cytochem.14, 291–299 (1966).Google Scholar
  8. Holzman, E.: Lysosomes in the physiology and pathology of neurons. In: Lysosomes in biology and pathology I, pp. 192–216. Amsterdam: North Holland Publ. Comp. 1969.Google Scholar
  9. Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol.27, 137 A (1965).Google Scholar
  10. Klüver, H., Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Neuropath. exp. Neurol.12, 400–403 (1953).Google Scholar
  11. Kristensson, K.: Transport of fluorescent protein tracer in peripheral nerves. Acta neuropath. (Berl.)16, 293–300 (1970).Google Scholar
  12. —, Olsson, Y.: Retrograde axonal transport of protein. Brain Res.29, 363–365 (1971a).Google Scholar
  13. ——: Uptake and retrograde axonal transport of peroxidase in hypoglossal neurons. Electron microscopical localization in the neuronal perikaryon. Acta neuropath. (Berl.)19, 1–9 (1971b).Google Scholar
  14. ——, Sjöstrand, J.: Axonal uptake and retrograde transport of exogenous proteins in the hypoglossal nerve. Brain Res.32, 399–406 (1971).Google Scholar
  15. Palmgren, A.: A rapid method for selective silver staining of nerve fibres and nerve endings in mounted paraffin sections. Acta zool.29, 377–392 (1948).Google Scholar
  16. Richardson, K. C., Jarrett, L., Finke, E. H.: Embedding in epoxy resin for ultrathin sectioning in electron microscopy. Stain Technol.35, 313–323 (1960).Google Scholar
  17. Steinwall, O., Klatzo, I.: Selective vulnerability of the blood-brain barrier in chemically induced lesions. J. Neuropath. exp. Neurol.25, 542–559 (1966).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Krister Kristensson
    • 1
  • Yngve Olsson
    • 1
    • 2
  1. 1.Neuropathological Laboratory, Department of PathologyUniversity of GöteborgGöteborgSweden
  2. 2.Patol. inst.UppsalaSweden

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