Uptake and retrograde axonal transport of protein tracers in hypoglossal neurons
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Protein tracers (albumin labelled with Evan's blue and horseradish peroxidase) accumulate in the cytoplasm of hypoglossal neurons in the brain stem following injection into the tongue of rats and mice resulting apparently from axonal uptake and subsequent retrograde transport from the periphery. The present study was performed to follow the fate of the protein tracers and to find out if they induce any signs of neuronal necrosis.
The proteins remained in the nerve cell bodies for about 6–11 days as revealed by fluorescence microscopy and light microscopical enzyme histochemistry after the injection into the tongue. Thereafter the proteins disappeared from the neurons presumably as a result of lysosomal degradation. The incorporation of foreign proteins did not produce as observed by light and electron microscopy any signs of cell degeneration.
Key wordsRetrograde Axonal Transport Neuronal Degradation Foreign Proteins
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- Brightman, M. W., Klatzo, I., Olsson, Y., Reese, T. S.: The blood-brain barrier to proteins under normal and pathological conditions. J. Neurol. Sci.10, 215–239 (1970).Google Scholar
- Brownstone, Y. S., Chapman-Andresen, C.: Degradation of endocytosed albumin by Chaos chaos. C. R. Carlsberg38, 297–313 (1971).Google Scholar
- Dales, S., Silverberg, H.: Viropexis of herpes simplex virus by HeLa cells. Virology37, 475–480 (1969).Google Scholar
- Ehrenreich, B. A., Cohn, Z. A.: The uptake and digestion of iodinated human serum albumin by macrophages in vitro. J. exp. Med.126, 941–958 (1967).Google Scholar
- Friede, R. L.: Topographic brain chemistry. New York-London: Academic Press 1966.Google Scholar
- Gabathuler, M.-P., Ryser, H. J.-P.: The digestive function of lysosomes as studied by the turnover of ingested foreign macromolecules. Proc. roy. Soc. B173, 95–98 (1969).Google Scholar
- Graham, R. C., Karnovsky, M. J.: The early stages of absorbtion of injected horseradish peroxidase in the proximal tubules of mouse kidney; Ultrastructural correlates by a new technique. J. Histochem. Cytochem.14, 291–299 (1966).Google Scholar
- Holzman, E.: Lysosomes in the physiology and pathology of neurons. In: Lysosomes in biology and pathology I, pp. 192–216. Amsterdam: North Holland Publ. Comp. 1969.Google Scholar
- Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol.27, 137 A (1965).Google Scholar
- Klüver, H., Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Neuropath. exp. Neurol.12, 400–403 (1953).Google Scholar
- Kristensson, K.: Transport of fluorescent protein tracer in peripheral nerves. Acta neuropath. (Berl.)16, 293–300 (1970).Google Scholar
- —, Olsson, Y.: Retrograde axonal transport of protein. Brain Res.29, 363–365 (1971a).Google Scholar
- ——: Uptake and retrograde axonal transport of peroxidase in hypoglossal neurons. Electron microscopical localization in the neuronal perikaryon. Acta neuropath. (Berl.)19, 1–9 (1971b).Google Scholar
- ——, Sjöstrand, J.: Axonal uptake and retrograde transport of exogenous proteins in the hypoglossal nerve. Brain Res.32, 399–406 (1971).Google Scholar
- Palmgren, A.: A rapid method for selective silver staining of nerve fibres and nerve endings in mounted paraffin sections. Acta zool.29, 377–392 (1948).Google Scholar
- Richardson, K. C., Jarrett, L., Finke, E. H.: Embedding in epoxy resin for ultrathin sectioning in electron microscopy. Stain Technol.35, 313–323 (1960).Google Scholar
- Steinwall, O., Klatzo, I.: Selective vulnerability of the blood-brain barrier in chemically induced lesions. J. Neuropath. exp. Neurol.25, 542–559 (1966).Google Scholar