Acta Neuropathologica

, Volume 51, Issue 1, pp 79–84 | Cite as

Cerebral lesions in rats with streptozotocin-induced diabetes

  • Noritsugu Mukai
  • Sadao Hori
  • Marsha Pomeroy
Original Works

Summary

We studied cerebral lesions in the streptozotocin-diabetic rat, an established model of diabetic retinopathy. A group of diabetic rats all had cerebral lesions, but showed no gross abnormalities of the brain or signs of effects on the central nervous system. Light and electron microscopy of horizontal brain sections stained by a variety of methods showed focal accumulation of collagen fibrils in the basement membranes of arteriole and capillary walls, slight degeneration of random neuronic cells (mainly in the brain stem), and no evidence of perivascular myelin pallor plaques. A marked tendency toward platelet aggregation, which leads to thrombosis, was also seen. There was no difference between results from rats killed 9 and 12 months after streptozotocin injection. The brain lesions bore a close resemblance to those seen in a nutritional encephalopathy known to be induced by a diet deficient in tocopherol and rich in oxidized oil, but differed from that encephalopathy in that the peculiar hypertrophic cerebral astroglia associated with diabetes resembled Alzheimer Type II astroglia. The differences between these cerebral astroglia and the retinal Müller cells may account for the difference in degree between the effects of diabetes on the brain and the retina.

Key words

Streptozotocin Wistar inbred rats Diabetes Cerebral vascular lesions Collagen fibrils Alzheimer Type II astroglia 

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References

  1. Adams CWM (1965) Histochemistry of the cells in the nervous system. In: Adams CWM (ed) Neurohistochemistry. Elsevier, Amsterdam London New York, pp 253–331Google Scholar
  2. Clements RS, Jr (1979) Diabetic neuropathy—New concepts of its etiology. Diabetes 28:604–611Google Scholar
  3. Dror Y, Budowski P, Bubis JJ, Sandbank U, Wolman M (1976) Chick nutritional encephalopathy induced by diet rich in oxidized oil and deficient in tocopherol. In: Zimmerman HM (ed) Progress in neuropathology, vol. III. Grune & Stratton, New York San Francisco London, pp 343–357Google Scholar
  4. Engerman RL (1976) Animal models of diabetic retinopathy. Trans Am Acad Ophthalmol Otolaryngol 81:710–715Google Scholar
  5. Gutierrez JA, Norenberg MD (1975) Alzheimer II astrocytosis following methionine sulfoximine. Arch Neurol 32:123–126Google Scholar
  6. Hori S, Mukai N (1980) Ultrastructural lesions of retinal pericapillary Müller cells in streptozotocin-induced diabetic rats. Albrecht Von Graefes Arch Klin Exp Ophthalmol 213:1–9Google Scholar
  7. Hori S, Nishida T, Mukai N (in press) Ultrastructural studies on lysosomes in retinal Müller cells of streptozotocin-diabetic rats. Invest Ophthalmol Vis SciGoogle Scholar
  8. Hirano A (1976) An outline of neuropathology. Igaku-Shoin, TokyoGoogle Scholar
  9. Junod A, Lambert AE, Stauffacher W, Renold AE (1969) Diabetogenic action of streptozotocin: Relationship of dose to metabolic response. J Clin Invest 48:2129–2139Google Scholar
  10. Kuwabara T (1965) Some aspects of retinal metabolism revealed by histochemistry. Müller cells in the pathological condition. In: Graymore CN (ed) Biochemistry of the retina. Academic Press, London New York, pp 93–99Google Scholar
  11. Mukai N (1975) A quick supra-vital staining method for the retinal Müller-fiber network under normal and pathological conditions. Brain Nerve 27:545–549Google Scholar
  12. Murata T (1979) Lipid peroxide formation in retina of alloxandiabetic rats. (unpubl. observ.)Google Scholar
  13. Plum F (1960) The neurologic complications of diabetes mellitus. In: Williams RH (ed) Diabetes. Hoeber/Harper & Row, New York, pp 602–622Google Scholar
  14. Rakieten N, Rakieten ML, Nadkarni MR (1963) Studies on the diabetogenic action of streptozotocin (NSC-37917). Cancer Chemother Rep 29:91–98Google Scholar
  15. Seitelberger F (1973) Zentrale pontine Myelinolyse. Schweiz Arch Neurol Neurochir Psychiatr 112:285–297Google Scholar
  16. Slater TF (1965) Notified contribution to discussion: More thoughts on ubiquinones. In: Graymore CN (ed) Biochemistry of the retina. Academic Press, London New York, pp 163–164Google Scholar
  17. Spiro, RG (1969) Glycoproteins: Their biochemistry, biology and role in human disease. N Engl J Med 281:991–1001, 1043–1056Google Scholar
  18. Towfighi J (1978) Vitamin E deficiency and “dying back” axonal lesions in the central and peripheral nervous system (CNS and PNS). J Neuropathol Exp Neurol 37:700 (abstr)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Noritsugu Mukai
    • 1
    • 2
  • Sadao Hori
    • 1
  • Marsha Pomeroy
    • 1
  1. 1.Wesley C. Bowers Laboratory of Pharmacology and Experimental PathologyEye Research Institute of Retina FoundationBostonUSA
  2. 2.Neuro-ophthalmology and Neuropathology at Harvard Medical SchoolBostonUSA

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