Acta Neuropathologica

, Volume 50, Issue 3, pp 181–186 | Cite as

Immunolabeling of SSPE and wild-type measles viruses in ferret brain cell culture

  • Hannah R. Brown
  • Halldor Thormar
  • Fu Hai Lin
Original Works


Immunocytochemical studies using horseradish peroxidase labeled antibody were undertaken in an attempt to determine whether there are detectable antigenic differences which correlate with the biological properties of different strains of SSPE and wild-type measles virus grown in ferret brain cell cultures. The rabbit anti-measles hyperimmune serum used in this experiment contained antibodies to all the measles virus proteins when tested by immunoprecipitation. When cells infected with the wild-type measles or productive SSPE virus strains were treated with this serum, heavy deposits of reaction product were seen on the cell membrane and virion envelope. When SSPE serum which contained relatively little antibody to the M protein was applied, a clear unlabeled area was evident just beneath the surface label. Cells infected with the non-productive SSPE strains were labeled by both sera in a spotty or discontinuous pattern on the outer surface of the cell membrane. The differences in membrane labeling seem to reflect differences in the expression of viral membrane proteins by the various SSPE and measles virus strains.

Key words

Subacute sclerosing panencephalitis Measies Immunoperoxidase Ferret brain cells 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Avrameas S, Ternynck T (1971) Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175–1179Google Scholar
  2. Brown HR, Thormar H (1976) A non-productive subacute sclerosing panencephalitis (SSPE) virus of human and ferret: an ultrastructural study. Acta Neuropathol (Berl) 34:339–351Google Scholar
  3. Brown HR, Thormar H (1977) Antibody in SSPE serum and brain IgG against measles virus smooth nucleocapsids detected by immunoelectron microscopy. Acta Neuropathol (Berl) 40:183–188Google Scholar
  4. Brown HR, Jervis GA, Thormar H (1977) Ultrastructural and histological studies of brains of ferrets inoculated with subacute sclerosing panencephalitis: similarities to human disease. J Neuropathol Exp Neurol 36:653–665Google Scholar
  5. Dubois-Dalcq M, Barbosa LH (1973) Immunoperoxidase stain of measles antigen in tissue culture. J Virol 12:909–918Google Scholar
  6. Dubois-Dalcq M, Barbosa LH, Hamilton R, Sever JL (1974) Comparison between productive and latent subacute sclerosing panencephalitis viral infection in vitro. Lab Invest 30:241–250Google Scholar
  7. Graves MC, Silver SM, Choppin PW (1978) Measles virus polypeptide synthesis in infected cells. Virology 86:254–263Google Scholar
  8. Hall WW, Choppin PW (1979) Evidence for lack of synthesis of the M polypeptide of measles virus in brain cells in subacute sclerosing panencephalitis. Virology 99:443–447Google Scholar
  9. Hall WW, Lamb RA, Choppin PW (1979) Measles and subacute sclerosing panencephalitis virus proteins: Lack of antibodies to the M protein in patients with subacute sclerosing panencephalitis. Proc Natl Acad Sci USA 76:2047–2051Google Scholar
  10. Lamb RA, Etkind PR, Choppin PW (1978) Evidence for a ninth influenza viral polypeptide. Virology 91:60–78Google Scholar
  11. Lin FH, (1978) Polyacrylamide gel electrophoresis of visna virus polypeptides isolated by agarose gel chromatography. J Virol 25:207–214Google Scholar
  12. Lin FH, Thormar H (1980) Absence of M protein in a cell-associated subacute sclerosing panencephalitis virus. Nature (in press)Google Scholar
  13. McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083Google Scholar
  14. Rott R (1979) Molecular basis of infectivity and pathogenicity of myxovirus. Arch Virol 59:285–298Google Scholar
  15. Thormar H, Jervis GA, Karl SC, Brown HR (1973) Passage in ferrets of encephalitogenic cell-associated measles virus isolated from brain of patient with subacute sclerosing panencephalitis. J Infect Dis 127:678–685Google Scholar
  16. Thormar H, Mehta PD, Brown HR (1978) Comparison of wild-type and subacute sclerosing panencephalitis strains of measles virus. Neurovirulence in ferrets and biological properties in cell cultures. J Exp Med 148:674–691Google Scholar
  17. Wechsler SL, Weiner HL, Fields BN (1979) Immune response in subacute sclerosing panencephalitis: Reduced antibody response to the matrix protein of measles virus. J Immunol 123:884–889Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Hannah R. Brown
    • 1
  • Halldor Thormar
    • 1
  • Fu Hai Lin
    • 1
  1. 1.N.Y.S. Institute for Basic Research in Mental RetardationStaten IslandUSA

Personalised recommendations