Immunocytochemistry of cerebellar astrocytomas: with a special note on rosenthal fibres
- 37 Downloads
Two astrocytic immunocytochemical markers, glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS), were demonstrated in cerebellar astrocytomas. The staining pattern for both antigens was similar, but GFAP demonstrated astrocytic processes better, while GS staining was stronger in poorly fibrillated cells. The various astrocytic forms, bipolar, stellate, bi- and multinucleate cells, displayed different immunostaining.
Rosenthal fibres also showed a varying pattern of reaction: larger fibres were entirely negative or had a narrow peripheral ring of dense reaction product, but occasional smaller fibres were positive. The varying proportion of filamentous and amorphous material within Rosenthal fibres could be responsible for this finding. The pale granular bodies possessed a GFAP-positive limiting membrane and GS-positive contents, suggestive of their astrocytic origin. Immunohistochemistry of GFAP and GS has contributed to the knowledge of cellular differentiation and secondary changes in cerebellar astrocytomas.
Key wordsCerebellar astrocytomas Immunocytochemistry Glial fibrillary acidic protein Glutamine synthetase Rosenthal fibres
Unable to display preview. Download preview PDF.
- Austin EJ (1984) Prognosis of cerebellar astrocytoma. J Neuropathol Exp Neurol 43:339Google Scholar
- Bonnin JM, Rubinstein LJ (1984) Immunohistochemistry of central nervous system tumors. J Neurosurg 60:1121–1133Google Scholar
- Deck, JHN, Eng LF, Bigbee J, Woodcock SM (1978) The role of glial fibrillary acidic protein in the diagnosis of central nervous system tumors. Acta Neuropathol (Berl) 42:183–190Google Scholar
- Eng LF, DeArmond SJ (1983) Immunocytochemistry of the glial fibrillary acidic protein. In: Zimmerman HM (ed) Progress in neuropathology, vol 5. Raven Press, New York, pp 19–39Google Scholar
- Eng LF, Rubinstein LJ (1978) Contribution of immunohistochemistry to diagnostic problems of human cerebral tumors. J Histochem Cytochem 26:513–522Google Scholar
- Herndon RM, Runbinstein LJ, Freeman JM, Mathieson G (1970) Light- and electron-microscopic observations on Rosenthal fibres in Alexander's disease and in multiple sclerosis. J Neuropathol Exp Neurol 29:524–551Google Scholar
- Heyderman E (1979) Immunoperoxidase technique in histopathology: applications, methods, and controls. J Clin Pathol 32:971–978Google Scholar
- Horoupian DS, Kress Y, Yen S-H, Gaskin F (1982) Nickelinduced changes and reappraisal of Rosenthal fibres in focal CNS lesions. J Neuropathol Exp Neurol 41:664–675Google Scholar
- Janzer RC, Friede RL (1981) Do Rosenthal fibres contain glial fibrillary acidic protein? Acta Neuropathol (Berl) 55:75–76Google Scholar
- Marsden HB, Kumar S, Kahn J, Anderton BJ (1983) A study of glial fibrillary acidic protein (GFAP) in childhood brain tumours. Int J Cancer 31:439–445Google Scholar
- Norenberg MD (1979) The distribution of glutamine synthetase in the rat central nervous system. J Histochem Cytochem 27:756–762Google Scholar
- Papasozomenos SC (1983) Glial fibrillary acidic (GFA) proteincontaining cells in the human pineal gland. J Neuropathol Exp Neurol 42:391–408Google Scholar
- Pilkington GJ, Lantos PL (1982) The role of glutamine synthetase in the diagnosis of cerebral tumours. Neuropathol Appl Neurobiol 8:227–236Google Scholar
- Russell DS, Rubinstein LJ (1977) Pathology of tumours of the nervous system, 4th edn. Arnold, LondonGoogle Scholar
- Towfighi J, Young R, Sassani J, Ramer J, Horoupian DS (1983) Alexander's disease: further light- and electron-microscopic observations. Acta Neuropathol (Berl) 61:36–42Google Scholar
- Van der Meulen JDM, Houthoff HJ, Ebels EJ (1978) Glial fibrillary acidic protein in human gliomas. Neuropathol Appl Neurobiol 4:177–190Google Scholar
- Velasco ME, Dahl D, Roessmann U, Gambetti P (1980) Immunohistochemical localization of glial fibrillary acidic protein in human glial neoplasms. Cancer 45:484–494Google Scholar