Journal of comparative physiology

, Volume 136, Issue 1, pp 39–43 | Cite as

Oxygen consumption in the rectal gland of the dogfishScyliorhinus canicula and the effects of cyclic AMP

  • T. J. Shuttleworth
  • J. L. Thompson
Article

Summary

Oxygen consumption was measured in slices of dogfish (Scyliorhinus canicula) rectal gland tissue and compared with spleen and kidney. Concentrations of dibutyryl cAMP and theophylline that have previously been shown to stimulate secretion from the perfused gland and to produce a large increase in ouabain binding in this tissue, greatly increased oxygen consumption in the rectal gland but were without effect on oxygen consumption in the spleen and the kidney. Addition of theophylline alone increased rectal gland oxygen consumption and no further increase was detected on addition of cAMP at the concentration used (0.05 mmol l−1).

Ouabain (10−4 M) significantly decreased oxygen consumption in all three tissues studied and completely abolished the increase in oxygen consumption of the rectal gland produced as a result of the addition of cAMP and theophylline.

These findings are discussed in relation to the apparent specific effect of cAMP on the rectal gland and its possible mode of action in this tissue.

Keywords

Oxygen Oxygen Consumption Human Physiology Theophylline Specific Effect 

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References

  1. Burger, J.W.: Further studies on the function of the rectal gland in the spiny dogfish. Physiol. Zool.35, 205–217 (1962)Google Scholar
  2. Burger, J.W.: Roles of the rectal gland and the kidneys in salt and water excretion in the spiny dogfish. Physiol. Zool.38, 191–196 (1965)Google Scholar
  3. Burger, J.W., Hess, W.N.: Function of the rectal gland in the spiny dogfish. Science131, 670–671 (1960)Google Scholar
  4. Butler, P.J., Taylor, E.W.: The effect of progressive hypoxia on respiration in the dogfish (Scyliorhinus canicula) at different seasonal temperatures. J. Exp. Biol.63, 117–130 (1975)Google Scholar
  5. Hilden, S., Hokin, L.E.: Active potassium transport coupled to active sodium transport in vesicles reconstituted from purified sodium and potassium ion-activated adenosine triphosphatase from the rectal gland ofSqualus acanthias. J. Biol. Chem.250, 6296–6303 (1975)Google Scholar
  6. Kyte, J.E.: Physiological consequences of the cellular distribution of sodium-plus-potassium ion dependent adenosine triphosphatase. Biochem. Soc. Trans.6, 833–836 (1978)Google Scholar
  7. Martin, D.W., Diamond, J.M.: Energetics of coupled active transport of sodium and chloride. J. Gen. Physiol.50, 295–315 (1966)Google Scholar
  8. McIlwain, H., Buddle, M.L.: Techniques in tissue metabolism. I. A mechanical chopper. Biochem. J.53, 412–420 (1953)Google Scholar
  9. Palmer, R.F.:In vitro perfusion of the isolated rectal gland ofSqualus acanthias. Clin. Res.14, 77 (1966)Google Scholar
  10. Potts, W.T.W.: Ion transport and osmoregulation in marine fish. In: Perspectives in experimental biology, Vol. 1. Spencer Davies, P. (ed.), pp. 65–75. Oxford, New York: Pergamon Press 1976Google Scholar
  11. Shuttleworth, T.J. Thompson, J.L.: Cyclic AMP and ouabainbinding sites in the rectal gland of the dogfishScyliorhinus canicula. J. Exp. Zool.206, 297–302 (1978)Google Scholar
  12. Siegel, N.J., Silva, P., Epstein, F.H., Maren, T.H., Hayslett, J.P.: Functional correlates of the dogfish rectal gland during in vitro perfusion. Comp. Biochem. Physiol.51A, 593–597 (1975)Google Scholar
  13. Silva, P., Stoff, J., Field, M., Fine, L., Forrest, J.N., Epstein, F.H.: Mechanism of active chloride secretion by shark rectal gland: role of Na-K-ATPase in chloride transport. Am. J. Physiol.233, 298–306 (1977)Google Scholar
  14. Solomon, R., Hays, L., Epstein, S., Forrest, J., Silva, P., Epstein, F.H.: Studies of rectal gland function inSqualus acanthias in vivo and in vitro. Bull. Mt. Desert Isl. Biol. Lab.14, 122–126 (1974)Google Scholar
  15. Stoff, J.S., Silva, P., Field, M., Forrest, J., Stevens, A., Epstein, F.H.: Cyclic AMP regulation of active chloride transport in the rectal gland of marine elasmobranchs. J. Exp. Zool.199, 443–448 (1977)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • T. J. Shuttleworth
    • 1
  • J. L. Thompson
    • 1
  1. 1.Department of Biological SciencesUniversity of ExeterExeterUK

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