Acta Neuropathologica

, Volume 66, Issue 3, pp 199–207 | Cite as

Effect of chlorpromazine on the localization of cAMP phosphodiesterase

  • L. C. Seegers
  • M. Haag
  • O. J. van Heerden
  • W. S. Joubert
  • J. J. Theron
Original Works

Summary

Chlorpromazine (CPZ) at dosages of 10 mg/kg body weight (b. wt.) affected the cytochemical localization of cAMP-dependent phosphodiesterase (cAMP PDE) activity in the synapses of the rat frontal cortex. Postsynaptic cAMP PDE activity was inhibited, and presynaptic activity increased. CPZ also inhibited membrane-bound ATPase activity in the frontal cortex. The activity of Na+−K+-ATPase was significantly (P<0.005) inhibited in isolated plasma membranes from the rat frontal cortex. CPZ exposure also affected the cytochemical localization of cations with potassium pyroantimonate. Precipitate, which could be removed with 5 mm EGTA, was decreased in the mitochondria and synaptic vesicles in presynaptic areas after CPZ treatment. The incorporation of45Ca2+ into slices of the rat frontal cortex was also significantly (P<0.001) inhibited by CPZ. This ultrastructural study shows that CPZ may affect biochemical events in an opposite manner in the pre- and post-synaptic areas of some neurons of the frontal cortex.

Key words

Chlorpromazine cAMP-dependent phosphodiesterase ATPase Synapses 45Ca2 incorporation 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • L. C. Seegers
    • 1
  • M. Haag
    • 1
  • O. J. van Heerden
    • 1
  • W. S. Joubert
    • 1
  • J. J. Theron
    • 1
  1. 1.Dept. of PhysiologyUniversity of PretoriaPretoriaSouth Africa

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