Acta Neuropathologica

, Volume 74, Issue 1, pp 81–83 | Cite as

Topochemical demonstration of zinc in meningiomas

  • I. Loennecken
  • W. Müller
  • R. Firsching
Regular Papers


In agreement with the atomic absorption tests, histochemical demonstration of zinc in meningiomas using the dithizone method revealed a higher level of zinc in fibromatous meningiomas than in its meningiotheliomatous variant. Calcified psammoma bodies and vessels exhibited an intensely positive reaction on their surface. The metabolic function of zinc in the synthesis of fibre proteins, as well as in metalloenzymes associated with mineralization, explains these findings.

Key words

Meningioma Zinc Psammoma bodies Calcified vessels 


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  1. Angyal AM, Archer GT (1968) The zinc content of rat mast cells. Aust J Exp Biol Med Sci 46:119–121Google Scholar
  2. Babcock AK, Henkin RI, Aamodt RL, Foster DM, Berman M (1982) Effects of oral zinc loading on zinc metabolism in humans. II. In vivo kinetics. Metabolism 31:336–347Google Scholar
  3. Bersin T (1963) Biochemie der Mineral- und Spurenelemente. Akademische Verlagsgesellschaft, Wiesbaden, pp 347–361Google Scholar
  4. Duckett S, Galle P, Escourolle R, Poirier J, Hauw JJ (1977) Presence of zinc, aluminum, magnesium in striopalledodentate (SPD) calcifications (Fahr's disease): electron probe study. Acta Neuropathol (Berl) 38:7–10Google Scholar
  5. Fabiani A, Monticone GF, Schiffer D (1967) Histochemical study of hydrolytic enzymes in meningiomas. Psychiatr Neurol 154:95–105Google Scholar
  6. Foster DM, Aamodt RL, Henkin RI, Berman M (1979) Zinc metabolism in humans: a kinetic model. Am J Physiol 237:340–349Google Scholar
  7. Höck A, Demmel U, Schicha H, Kasperek K, Feinendegen LE (1975) Trace element concentration in human brain. Activation analysis of cobalt, iron, rubidium, selenium, zinc, chromium, silver, cesium, antimony and scandium. Brain 98:49–64Google Scholar
  8. Kubota T, Hirano A, Sato K, Yamamoto S (1985) Fine structure of psammoma bodies at the outer aspect of blood vessels in meningioma. Acta Neuropathol (Berl) 66:163–166Google Scholar
  9. Leuthardt F (1977) Intermediär-Stoffwechsel. Walter de Gruyter, Berlin New York, pp 273–274Google Scholar
  10. Mager M, McNary WF, Lionetti F (1953) The histochemical detection of zinc. J Histochem Cytochem 1:493–504Google Scholar
  11. Michotte Y, Smeyers-Verbeke J, Massart DL, Lowenthal A (1973) Morphological, structural and chemical study of small calcifications. Acta Neurol Belg 73:374–379Google Scholar
  12. Müller W, Iffland R (1981) Studies on metals in meningiomas by atomic absorption spectrometry. Acta Neuropathol (Berl) 55:53–58Google Scholar
  13. Osske G, Jänisch W (1967) Zur Enzymhistochemie der Meningiome. Acta Neuropathol (Berl) 9:290–297Google Scholar
  14. Pihl E, Gustafson GT (1967) Heavy metals in rat mast cell granules. Lab Invest 17:588–598Google Scholar
  15. Rizzoli HV, Randall JD, Smith DR (1978) Psammoma bodies in meningioma. Appearance by scanning electron microscopy. Virchows Arch [A] 380:317–325Google Scholar
  16. Roinel N, Morel F, Istin M (1973) Etude des granules calcifiés du manteau des lamellibranches à l'aide de la microscope électronique. Calcif Tissue Res 11:163–170Google Scholar
  17. Samachson J, Dennis J, Fowler R, Schmitz A (1967) The reaction of65Zn with the surfaces of bone and bone mineral. Biochim Biophys Acta 148:767–773Google Scholar
  18. Schicha H, Müller W, Kasperek K, Schröder R (1974) Neutronenaktivierungsanalytische Bestimmung der Spurenelemente Kobalt, Eisen, Rubidium, Selen, Zink, Chrom, Silber, Caesium, Antimon und Scandium in operativ entnommenen Hirntumoren des Menschen. Beitr Pathol 151:281–296Google Scholar
  19. Schiffer D (1971) Calcification in nervous tissue. In: Minckler J (ed) Pathology of the nervous system, vol 2. McGraw-Hill, New York, pp 1342–1360Google Scholar
  20. Schmidt R, Rautschke R (1963) Die Dithizon-Zink-Reaktion in der Histochemie. Acta Histochem 15:87–112Google Scholar
  21. Simkiss K (1979) Metal ions in cells. Endeavour 3:2–6Google Scholar
  22. Ulmer D (1977) Trace elements. N Engl J Med 297:318–321Google Scholar
  23. Yoshinaga T, Shinji Y, Katayama T, Yamamoto Y (1965) Über die Darstellungsmethode des Zinks im Gewebe und ihren Mechanismus. Acta Histochem 21:276–283Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • I. Loennecken
    • 1
  • W. Müller
    • 1
  • R. Firsching
    • 2
  1. 1.Department of Pathology, UniversityKöln 41Federal Republic of Germany
  2. 2.Department of Neurosurgery, UniversityKöln 41Federal Republic of Germany

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