Cancer Chemotherapy and Pharmacology

, Volume 37, Issue 3, pp 203–210 | Cite as

A microcomputer program for calculating cell population doubling time in vitro and in vivo

  • Jeffrey I. Zwicker
  • Robert T. Proffitt
  • C. Patrick Reynolds
Cell Growth Kinetics, Nude Mice, Microcomputer, Computer Software


Determination of the doubling time for a population of cells can involve tedious calculations. We have developed computer software for MS-DOS microcomputers to expedite the analysis of tumor cell growth in vitro and in vivo. This program, DOUBLE-TIME, assists in the collection of cell numbers into a database and calculates the doubling time for a population of cells from the plot of cell growth over time. For experiments where tumor mass is measured in vivo, the software collects measurements of tumor size, calculates tumor volume (mass), generates growth curves for tumor volume change over time, and determines the doubling time of the tumor and the mean for multiple tumors. DOUBLE-TIME plots both total and viable cell numbers over time, calculates standard error of the doubling time, and the doubling time for a selected portion of a growth curve. This software also automates the cell counting process with a software-generated cell counter that allows cell counts to be tallied directly into the computer via a mouse.

Key words

Cell growth kinetics Nude mice Microcomputer Computer software 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Jeffrey I. Zwicker
    • 1
    • 2
  • Robert T. Proffitt
    • 1
    • 2
  • C. Patrick Reynolds
    • 1
    • 2
  1. 1.Departments of Pediatrics and PathologyUniversity of Southern California School of MedicineLos AngelesUSA
  2. 2.Division of Hematology-OncologyChildren's Hospital Los AngelesLos AngelesUSA

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