Acta Neuropathologica

, Volume 78, Issue 4, pp 429–437 | Cite as

Immunodetection of the amyloid P component in Alzheimer's disease

  • T. Duong
  • E. C. Pommier
  • A. B. Scheibel
Regular Papers


Amyloid P component (AP), a plasma constituent normally not found in brain arenchyma, has been immunohistochemically determined in brains from patients with Alzheimer's disease (AD). Tissue came from 11 clinically diagnosed and neuropathologically verified AD patients and from 6 normal aged controls. Positive labeling for AP was observed in amyloidotic blood vessels, senile plaques (SP) and neurofibrillary tangles (NFT). The immunoreactivity was specific for these AD-associated lesions and clearly revealed their morphological appearance. Affected blood vessels appeared to be mainly of the arteriolar types and were labeled abluminally in short segments. SP constituents such as amyloid fibrils, anyloid core and degenerative axonal and dendritic processes were positive for AP antiserum; the morphology and distribution of immunoreactive SP corresponded to previous descriptions. Labeling of NFT revealed the morphology of paired helical and straight filaments. In all cerebral areas studied, tangle-bearing neurons were immunoreactive to AP antiserum, suggesting that AP is involved in early cellular development of NFT. Given the large molecular weight of AP (about 220,000), these results point to a potential impairment of the blood-brain barrier in AD. Since AP is always present in systemic amyloidosis, its detection in cerebral amyloidosis associated with AD may suggest mechanisms common to the two disorders.

Key words

Alzheimer's disease Amyloid P component Amyloidosis Neurofibrils 


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  1. 1.
    Baltz ML, Caspi D, Evans DJ, Rowe IF, Hind CRK, Pepys MB (1986) Circulating serum amyloid P component is the precursor of amyloid P component in tissue amyloid deposits. Clin Exp Immunol 66:691–700Google Scholar
  2. 2.
    Binette P, Binette M (1974) The isolation and identification of the P-component of normal human plasma proteins. Biochem J 143:253–254Google Scholar
  3. 3.
    Cathcart ES, Shirahama T, Cohen AS (1967) Isolation and identification of a plasma component of amyloid. Biochem Biophys Acta 147:392–393Google Scholar
  4. 4.
    Coria F, Castano E, Prelli F, Larrondo-Lillo M, Frangione B (1988) Isolation and characterization of amyloid P component from Alzheimer's disease and other types of cerebral amyloidosis. Lab Invest 58:454–458Google Scholar
  5. 5.
    Duyckaerts C, Hauw JJ, Bastenaire F, Piette F, Poulain C, Rainsard V, Javoy-Agid F, Berthaux P (1986) Laminar distribution of neocortical senile plaques in senile dementia of the Alzheimer type. Acta Neuropathol (Berl) 70:249–256Google Scholar
  6. 6.
    Elovaara I, Maury CPJ, Palo J (1986) Serum amyloid A component, albumin and prealbumin in Alzheimer's disease patients with Down's syndrome. Acta Neurol Scand 74:245–250Google Scholar
  7. 7.
    Husebekk A, Skogen B, Husby G, Marhaug G (1985) Transformation of amyloid precursor SAA to protein AA and incorporation in amyloid fibrils in vivo. Scand J Immunol 21:283–287Google Scholar
  8. 8.
    Hyman BT, Van Hoesen GW, Damasio AR, Barnes CL (1984) Alzheimer's disease: cell-specific pathology isolates the hippocampal formation. Science 225:1168–1170Google Scholar
  9. 9.
    Iseki E, Amano M, Matsuishi T, Yokoi S, Arai N, Yagishita S (1988) Case report. A case of familial, atypical Alzheimer's disease: immunohistochemical study of amyloid P-component. Neuropathol Appl Neurobiol 14:169–174Google Scholar
  10. 10.
    Khachaturian ZS (1985) Diagnosis of Alzheimer's disease. Arch Neurol 42:1097–1105Google Scholar
  11. 11.
    Kidd M, Allsop D, Landon M (1985) Senile plaque amyloid, paired helical filaments, and cerebrovascular amyloid in Alzheimer's disease are all deposits on the same protein [Letter to the Editor]. Lancet I:278Google Scholar
  12. 12.
    Kirschner DA, Abraham D, Selkoe DJ (1986) X-ray diffraction from intraneuronal paired helical filaments and extraneuronal amyloid fibers in Alzheimer disease indicates cross-β conformation. Proc Natl Acad Sci USA 83:503–507Google Scholar
  13. 13.
    Mann DMA, Tucker CM, Yates PO (1988) Alzheimer's disease: an olfactory connection? Mech Ageing Dev 42:1–15Google Scholar
  14. 14.
    Mann DMA, Yates PO, Marcyniuk B (1984) A comparison of changes in the nucleus basalis and locus caeruleus in Alzheimer's disease. J Neurol Neurosurg Psychiatry 47:201–203Google Scholar
  15. 15.
    Masters CL, Beyreuther K (1988) The blood-brain barrier in Alzheimer's disease and normal aging. Neurobiol Aging 9:43–44Google Scholar
  16. 16.
    Ohm TG, Braak H (1987) Olfactory bulb changes in Alzheimer's disease. Acta Neuropathol (Berl) 73:365–369Google Scholar
  17. 17.
    Osmand AP, Friedenson B, Gewurz H, Painter RH, Hofmann TH, Shelton E (1977) Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins). Proc Natl Acad Sci USA 74:739–743Google Scholar
  18. 18.
    Pearson RCA, Esiri MM, Hiorns RW, Wilcock GK, Powell TPS (1985) Anatomical correlates of the distribution of the pathological changes in the neocortex in Alzheimer's disease. Proc Natl Acad Sci USA 82:4531–4534Google Scholar
  19. 19.
    Pepys MB (1986) Amyloid P component: structure and properties. In: Marrink J, Van Rijswijk MH (eds) Amyloidosis. Martinus Nijhoff, Boston, pp 43–49Google Scholar
  20. 20.
    Pepys MB, DeBeer FC, Dyck RF, Holford S, Breathnack SM, Black MM, Tribe CRF, Evans DJ, Feinstein A (1982) Biology of serum amyloid P component. Ann NY Acad Sci 389:286–297Google Scholar
  21. 21.
    Prelli F, Pras M, Frangione B (1985) The primary structure of human tissue amyloid P component from patient with primary idiopathic amyloidosis. J Biol Chem 260:12895–12898Google Scholar
  22. 22.
    Rogers J, Morrison JH (1985) Quantitative morphology and regional and laminar distributions of senile plaques in Alzheimer's disease. J Neurosci 5:2801–2808Google Scholar
  23. 23.
    Rowe IF, Jensson O, Lewis PD, Candy J, Tennent GA, Pepys MB (1984) Immunohistochemical demonstration of amyloid P component in cerebrovascular amyloidosis. Neuropathol Appl Neurobiol 10:53–61Google Scholar
  24. 24.
    Saper CB, German DC, White III CL (1985) Neuronal pathology in the nucleus basalis and associated cell groups in Alzheimer's disease: possible role in cell loss. Neurology 35:1089–1095Google Scholar
  25. 25.
    Saperia D, Perlmutter LS, Athanikar J, Chui HC (1988) Amyloid P component in dementia. Soc Neurosci [Abstr] 14:638Google Scholar
  26. 26.
    Scheibel AB, Duong T, Tomiyasu U (1986) Microvascular changes in Alzheimer's disease. In: Scheibel AB, Wechsler AF (eds) The biological substrates of Alzheimer's disease. Academic Press, New York, pp 177–192Google Scholar
  27. 27.
    Scheibel AB, Duong T, Tomiyasu U (1987) Denervation microangiopathy in senile dementia, Alzheimer type. Alzheimer Dis Assoc Disorders 1:19–37Google Scholar
  28. 28.
    Scheibel AB, Pommier E, Duong T (1987) Immunodetection of human serum amyloid P component in Alzheimer's disease. Soc Neurosci [Abstr] 13:1152Google Scholar
  29. 29.
    Sternberger LA (1986) Immunocytochemistry. Wiley and Sons, New York, pp 90–245Google Scholar
  30. 30.
    Terry RD, Wisniewski HM (1970) The ultrastructure of the neurofibrillary tangle and the senile plaque. In: Wolstenholme GW, O'Connor MO (eds) Alzheimer's disease and related conditions. Churchill, London, pp 145–168Google Scholar
  31. 31.
    Westermark P, Shirahama T, Skinner M, Brun A, Cameron R, Cohen AS (1982) Immunohistochemical evidence for the lack of amyloid P component in some intercerebral amyloids. Lab Invest 46:457–460Google Scholar
  32. 32.
    Woo P, Korenberg JR, Whitehead ASJ (1985) Characterization of genomic and complementary DNA sequence of serum amyloid P component. J Biol Chem 260:13383–13388Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • T. Duong
    • 1
    • 3
  • E. C. Pommier
    • 1
    • 3
  • A. B. Scheibel
    • 1
    • 2
    • 3
  1. 1.Department of Anatomy and Cell BiologyUCLA Medical SchoolLos AngelesUSA
  2. 2.Department of PsychiatryUCLA Medical SchoolLos AngelesUSA
  3. 3.Brain Research InstituteUCLA Medical SchoolLos AngelesUSA

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