Acta Neuropathologica

, Volume 71, Issue 3–4, pp 251–258 | Cite as

Proliferation of Schwann cells in demyelinated rat sciatic nerve

  • K. Saida
  • T. Saida
Original Works


Experimental demyelination was induced by intraneural injection of anti-galctocerebroside serum into the sciatic nerves of rats. Schwann cells undergoing mitotic division were observed between days 3 to 9 after the injection and demyelinated segments were still associated with macrophages. Dividing Schwann cells were often present in association with both unmyelinated and myelinated fibers. Whether or not, daughter Schwann cells migrate along the same fiber towards neighboring demyelinated segments remains unclear. When Schwann cells attached to axon membranes of demyelinated segments were studied at later time points, they were present in clusters randomly at various regions of the segments. There was no proximo-distal gradient for the wave of Schwann cell proliferation. Mean Schwann cell internuclear distances were around 40–50 μm at the earliest time of remyelination. Schwann cell redistribution and remyelination progressed regardless of the length of demyelinated segments.

Key words

Schwann cells Demyelination Remyelination Mitosis Guillain-Barré syndrome 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ashbury AK (1975) The biology of Schwann cells. In: Dyck PJ, Thomas PK, Lambert EH (eds) Peripheral neuropathy, vol I. Saunders, Philadelphia, pp 201–212Google Scholar
  2. Billings-Gagliardi S, Webster HDeF, O'Connell MF (1974) In vivo and electron microscopic observations of Schwann cells in developing tadpole nerve fibers. Am J Anat 141:375–391Google Scholar
  3. Bonnaud-Toulze EN, Raine CS (1980) Remodelling during remyelination in the peripheral nervous system. Neuropathol Appl Neurobiol 6:279–290Google Scholar
  4. Bradley WG, Asbury AK (1970) Duration of synthesis phase in neurilemma cells in mouse sciatic nerve during degeneration. Exp Neurol 26:275–282Google Scholar
  5. Dyck PJ, Lais AC (1970) Electron microscopy of teased nerve fibers: method permitting examination of repeating structures of same fiber. Brain Res 23:418–424Google Scholar
  6. Friede RL, Johnstone MA (1967) Responses of thymidine labeling of nuclei in gray matter and nerve following sciatic transection. Acta Neuropathol (Berl) 7:218–231Google Scholar
  7. Gross MLP, Thomas PK (1981) The treatment of chronic relapsing and chronic progressive idiopathic inflammatory polyneuropathy by plasma axchange. J Neurol Sci 52:69–78Google Scholar
  8. Hall SM, Gregson NA (1978) The effect of 5-bromodeoxyuridine on the remyelination in the peripheral nervous system of the mouse. Neuropthol Appl Neurobiol 4:117–127Google Scholar
  9. Ignatius MJ, Chander CR, Shooter EM (1985) Nerve growth factor-treated, neurite-bearing PC 12 cells continue to synthesize DNA. J Neurosci 5:343–351Google Scholar
  10. Kaldron N (1984) Schwann cell proliferation and localized proteolysis: expression of plasminogen-activator activity predominates in the proliferating cell populations. Proc Natl Acad Sci USA 81:7216–7220Google Scholar
  11. Kawanishi T, Itagaki Y, Saida I, Motomura S, Yokota T, Matsuura T (1985) The treatment of chronic inflammatory demyelinating polyradiculoneuropathy by double-filtration plasmapheresis. In: Oda T (ed) Therapeutic plasmapheresis, vol IV. Schattauer, New York Stuttgart Berlin, pp 513–518Google Scholar
  12. King RHM, Pollard JD, Thomas PK (1975) Abberrant remyelination in chronic relapsing experimental allergic neuritis. Neuropathol Appl Neurobiol 1:367–378Google Scholar
  13. Lubinska L (1961) Sedentary and migratory states of Schwann cells. Exp Cell Res 8:74–90Google Scholar
  14. Said G, Duchett S, Sauron B (1981) Proliferation of Schwann cells in Tellurium-induced demyelination in young rats. A radioautographic and teased nerve fiber study. Acta Neuropath (Berl) 53:173–179Google Scholar
  15. Saida K, Saida T, Brown MJ, Silberberg DH (1979) In vivo demyelination induced by intraneural injection of antigalactocerebroside serum: a morphologic study. Am J Pathol 95:99–110Google Scholar
  16. Saida K, Summer AJ, Saida T, Brown MJ, Silberberg DH (1980) Antiserum-mediated demyelination: relationship between remyelination and functional recovery. Ann Neurol 8:12–24Google Scholar
  17. Saida K, Saida T, Kayama H, Nishitani H (1984) Rapid alterations of the axon membrane in antibody-mediated demyelination. Ann Neurol 15:581–589Google Scholar
  18. Saida T, Saida K, Dorfman SH, Silberberg DH, Sumner AJ, Manning MC, Lisak RP, Brown MJ (1979) Experimental allergic neuritis induced by sensitization with galactocerebroside. Science 204:1103–106Google Scholar
  19. Saida T, Saida K, Silberberg DH, Brown MJ (1981) Experimental allergic neuritis induced by galactocerebroside. Ann Neurol [Suppl] 9:87–101Google Scholar
  20. Salzer JL, Bunge RP (1980) Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration and direct injury. J Cell Biol 84:739–752Google Scholar
  21. Salzer JL, Bunge RP, Glaser L (1980a) Studies of Schwann cell proliferation. III. Evidence for the surface localization of the neurite mitogen. J Cell Biol 84:767–778Google Scholar
  22. Salzer JL, Williams AK, Glaser L, Bunge RP (1980b) Studies of Schwann cell proliferation. II. Characterization of the stimulation and specificity of the response to a neurite membrane fraction. J Cell Biol 84:753–766Google Scholar
  23. Server AC, Lefkowith J, Braine H, McKahann GM (1979) Treatment of chronic relapsing inflammatory polyradiculoneuropathy by plasma exchange. Ann Neurol 6:258–261Google Scholar
  24. Sobue G, Pleasure D (1984) Schwann cell galactocerebroside induced by derivatives of adenosine 3′,5′-monophosphate. Science 224:72–74Google Scholar
  25. Sobue G, Kreider B, Asbury AK, Pleasure D (1983) Specific and potent mitogenic effect of axolemmal fraction of Schwann cells from rat sciatic nerves in serum-containing and defined media. Brain Res 280:263–275Google Scholar
  26. Thomas GA (1948) Quantitative histology of Wallerian degeneration. II. Nuclear population in two nerves of different fiber spectrum. J Anat 82:135–145Google Scholar
  27. Toyka KV, Augspach R, Wiethölter H, Besinger UA, Haneveld F, Liebert UG, Heininger K, Schwendemann G, Reiners K, Grabensee B (1982) Plasma exchange in chronic inflammatory polyneuropathy: evidence suggestive of a pathogenic humoral factor. Muscle Nerve 5:479–484Google Scholar
  28. Wood PM, Bunge RP (1975) Evidence that sensory axons are mitogenic for Schwann cells. Nature 256:662–664Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • K. Saida
    • 1
  • T. Saida
    • 1
  1. 1.Department of Neurology and Clinical Research CenterUtano National HospitalKyotoJapan

Personalised recommendations