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Acta Neuropathologica

, Volume 71, Issue 1–2, pp 142–149 | Cite as

Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions

Morphological aspects
  • M. Westphal
  • P. Jaquet
  • Charles B. Wilson
Original Works

Summary

Tissues from 12 human corticotropin-secreting adenomas, obtained during transsphenoidal surgery for Cushing's disease (CD, ten cases) or Nelson's syndrome (NS, two cases), were mechanically dispersed. The resulting single cells and cell aggregates were plated on extracellular matrix derived from bovine corneal endothelia. CD and NS cells showed distinct morphological differences initially, CD cells being much more spherical than the flattened NS cells. By 10 days at the latest after plating, however, CD and NS cells were indistinguishable morphologically. Cultured cells from both entities responded with rounding to cortisol (hydrocortisone, 10−6M) within 4–6 h. Synthetic ovine corticotropin-releasing factor (10−8M) produced flattening and extension of cytoplasmic processes after as early as 2 h.

Key words

β-Endorphin Corticotropin-releasing factor Cushing's disease Nelson's syndrome Pituitary adenoma 

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References

  1. Bethea CL, Weiner RI (1981) Human prolactin secreting adenoma cells maintained on extracellular matrix. Endocrinology 108:357–360Google Scholar
  2. Cohen S, Savage CR Jr (1975) Preparation of epidermal growth factor. Methods Enzymol 37:424–430Google Scholar
  3. Denef C, Baes M (1982) β-Adrenergic stimulation of prolactin release from superfused pituitary cell aggregates. Endocrinology 111:356–358Google Scholar
  4. Fehm HL, Voigt KH, Kummer G, Lang R, Pfeiffer EF (1979) Differential and integral corticosteroid feedback effects on ACTH secretion in hypoadrenocorticism. J Clin Invest 63:247–253Google Scholar
  5. Gospodarowicz D, Fujii DK (1981) The extracellular matrix and the control of cell proliferation and differentiation. Miami Winter Symposia, vol 18. Academic Press, New York, pp 113–132Google Scholar
  6. Gospodarowicz D, Bialecki H, Greenburg G (1978) Purification of the fibroblast growth factor activity from bovine brain. J Biol Chem 253:3736–3744Google Scholar
  7. Gospodarowicz D, Hirabayashi K, Giguere L, Tauber JP (1981) Factors controlling the proliferative rate, final cell density, and lifespan of bovine vascular smooth muscle cells in culture. J Cell Biol 106:568–578Google Scholar
  8. Hardy J (1969) Transsphenoidal microsurgery of the normal and pathological pituitary. Clin Neurosurg 16:185–217Google Scholar
  9. Kleinman HK, Klebe RJ, Martin GR (1981) Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol 88:473–485Google Scholar
  10. Lüdecke DK, Bansemer J, Resetic J, Westphal M (1980) Different corticoid feedback effects in adenomatous and anterior lobe tissue in Cushing's disease. In: Faglia G, Giovanelli MA, MacLeod RM (eds) Pituitary microadenomas. Academic Press, London, pp 187–194Google Scholar
  11. Mulder GH, Krieger DT (1980) The release of ACTH by human pituitary and tumor cells in a superfusion system. In: Jutisz M, McKerns KW (eds) Synthesis and release of adenohypophyseal hormones. Plenum Press, New York London, pp 543–559Google Scholar
  12. Oki S, Nakao K, Tanaka J, Horii K, Nakai Y, Shimbo S, Watanabe M, Nakane T, Kuwayama A, Kageyama N, Imura H (1981) Concomitant secretion of adrenocorticotropin, β-endorphin and γ-melanotropin from perfused pituitary tumor cells of Cushing's disease: effects of lysine vasopressin, rat median eminence extracts, thyrotropin-releasing hormone, and luteinizing hormone-releasing hormone. J Clin Endocrinol Metab 52:42–49Google Scholar
  13. Oliva D, Nicosia S, Spada A, Giannattasio G (1982) VIP stimulates ACTH release and adenylate cyclase in human ACTH-secreting pituitary adenomas. Eur J Pharmacol 83:101–105Google Scholar
  14. Sharp B, Melmed S, Goldberg R, Carlson HE, Refetoff S, Hershman JM (1982) Radioimmunoassay detection of endorphins from long-term culture of human pituitary tumor cells. Acta Endocrinol Copenh 99:174–178Google Scholar
  15. Snyder J, Hymer WC, Wilfinger WW (1978) Culture of human pituitary prolactin and growth hormone cells. Cell Tiss Res 379:191Google Scholar
  16. Tramontano D, Avivi A, Ambesi-Impiombato FS, Barak L, Ciger B, Schlessinger J (1982) Thyrotropin induces changes in the morphology and the organization of microfilament structures in cultured thyroid cells. Exp Cell Res 137:269–272Google Scholar
  17. Weiner RI, Bethea CL, Jaquet P, Ramsdell JS, Gospodarowicz DJ (1983) Culture of dispersed anterior pituitary cells on extracellular matrix. Methods Enzymol 103:287–293Google Scholar
  18. Westphal M, Jaquet P, Wilson CB (1986) Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions. Secretory aspects. Acta Neuropathol (Berl) 71:111–118Google Scholar
  19. Wicha MS, Lowrie G, Kohn E, Bagavandoss P, Mahn T (1982) Extracellular matrix promotes mammary epithelial growth and differentiation in vitro. Proc Natl Acad Sci USA 79:3213–3217Google Scholar
  20. Zimmerman AE, Milligan JV, Joneja MG (1981) Scanning electron microscopy of rat neurointermediate lobe cells in culture. Acta Anat (Basel) 110:259–269Google Scholar
  21. Zor U (1983) Role of cytoskeletal organization in the regulation of adenylate cyclase-cyclic adenosine monophosphate by hormones. Endocr Rev 4:1–21Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • M. Westphal
    • 1
    • 2
  • P. Jaquet
    • 1
    • 2
  • Charles B. Wilson
    • 1
    • 2
  1. 1.Laboratory of Molecular Endocrinology, School of MedicineUniversity of CaliforniaSan FranciscoUSA
  2. 2.Departments of Gynecology and Reproductive Sciences, and Neurological Surgery, School of MedicineUniversity of CaliforniaSan FranciscoUSA

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