Acta Neuropathologica

, Volume 76, Issue 3, pp 287–291 | Cite as

Marek's disease virus-induced transient paralysis in chickens: alterations in brain density

  • D. E. Swayne
  • O. J. Fletcher
  • L. W. Schierman
Regular Papers


The presence of brain edema and its distribution in Marek's disease virus(MDV)-induced transient paralysis (TP) was determined in genetically resistant and susceptible inbred White Leghorn chickens. Density measurements on samples from the cerebellum, cerebral cortex, optic chiasm, and optic lobe were determined on continuous density Percoll gradients. At 9 days post inoculation of MDV, the cerebellums from the susceptible line of chickens were significantly less dense than cerebellums from the resistant line or the controls of either line. The cerebellar edema, as measured by tissue density, coincided with the transient neurological symptoms and was not present in brain tissue from clinically recovered TP birds.

Key words

Marek's disease herpesvirus Transient paralysis Percoll Density gradients Brain edema 


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  1. 1.
    Bickford AA (1964) Comparative study of meningoencephalitis syndrome and toxoplasmosis in chickens. MS thesis. Purdue University, Lafayette, Indiana (USA)Google Scholar
  2. 2.
    Bothe HW, Bodsch W, Hossmann KA (1984) Relationship between specific gravity, water content, and serum protein extravasation in various types of vasogenic brain edema. Acta Neuropathol (Berl) 64:37–42Google Scholar
  3. 3.
    Cho BR, Kenzy SG, Mathey WF (1970) Histologic and microbiologic studies of chickens with transient paralysis. Avian Dis 14:587–598Google Scholar
  4. 4.
    Gibbons JD (1985) Nonparametric methods for quantitative analysis, 2nd edn. American Sciences Press, Columbus, Ohio, pp 157–204Google Scholar
  5. 5.
    Hultstrom D, Malmgren L, Gilstring D, Olsson Y (1983) FITC-dextrans as tracers for macromolecular movements in the nervous system. A freeze-drying method for dextrans of various molecular sizes injected into normal animals. Acta Neuropathol (Berl) 59:53–62Google Scholar
  6. 6.
    Jungherr EL (1969) The neuroanatomy of the domestic fowl. Avian Dis (special issue):1–26Google Scholar
  7. 7.
    Kenzy SG, Cho BR, Kim Y (1973) Oncogenic Marek's disease herpesvirus in avian encephalitis (temporary paralysis). J Natl Cancer Inst 51:977–982Google Scholar
  8. 8.
    Kitz K, Lassmann H, Karcher D, Lowenthal A (1984) Blood-brain barrier in chronic relapsing experimental allergic encephalomyelitis: a correlative study between cerebrospinal fluid protein concentrations and tracer leakage in the central nervous system. Acta Neuropathol (Berl) 63:41–50Google Scholar
  9. 9.
    Kornegay JN, Gorgacz EJ, Parker MA, Duncan JR, Schierman LW (1983) Marek's disease virus-induced transient paralysis: a comparison of lesions in susceptible and resistant lines of chickens. Acta Neuropathol (Berl) 61:263–269Google Scholar
  10. 10.
    Lilliefors HW (1967) On the Kolmogorov-Smirnov test for normality with mean and variance unknown. Am Stat Assoc J 62:399–402Google Scholar
  11. 11.
    Miller JD (1979) The management of cerebral edema. Br J Hosp Med 21:152–165Google Scholar
  12. 12.
    Olsson Y, Kristensson K (1979) Recent applications of tracer techniques to neuropathology, with reference to vascular permeability and axonal flow. Adv Neuropathol 1:1–26Google Scholar
  13. 13.
    Parker MA, Schierman LW (1983) Suppression of humoral immunity in chickens prevents transient paralysis caused by a herpesvirus. J Immunol 130:2000–2001Google Scholar
  14. 14.
    Penn RD (1980) Cerebral edema and neurological function: CT, evoked response, and clinical examination. Adv Neurol 28:383–394Google Scholar
  15. 15.
    Purcell DA, Taylor SM (1971) The histopathology of transient paralysis of the fowl. Br Vet J 127:313–317Google Scholar
  16. 16.
    Schierman LW, Fletcher OJ (1980) Genetic control of Marek's disease virus-induced transient paralysis: association with the major histocompatibility complex. In: Biggs PM (ed) Resistence and immunity to Marek's disease. Commission of the European Communities, Brussels, pp 429–442Google Scholar
  17. 17.
    Schultz RL, Karlsson U (1965) Fixation of the central nervous system for electron microscopy by aldehyde perfusion. II. Effect of osmolality, pH of perfusate, and fixative concentration. J Ultrastruct Res 12:187–206Google Scholar
  18. 18.
    Settnes OP (1982) Marek's disease. A common naturally herpes virus-induced lymphoma of the chicken. Nord Vet Med [Suppl] II:11–132Google Scholar
  19. 19.
    Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. Iowa State University Press, Ames, Iowa, pp 175–193Google Scholar
  20. 20.
    Tengvar C, Forssen M, Hultstrom D, Olsson Y, Pertoft H, Pettersson A (1982) Measurement of edema in the nervous system: use of Percoll density gradients for determination of specific gravity in cerebral cortex and white matter under normal conditions and in experimental cytotoxic brain edema. Acta Neuropathol (Berl) 57:143–150Google Scholar
  21. 21.
    Tengvar C, Hultstrom D, Olsson Y (1983) An improved density gradient for measurements of experimental brain edema: addition of sucrose to an isotonic gradient in an attempt to balance osmotic conditions during density determinations. Acta Neuropathol (Berl) 61:201–206Google Scholar
  22. 22.
    Walker D, Gratton DAP (1968) A temporary paralytic syndrome of growing pullets. Vet Rec 82:43–47Google Scholar
  23. 23.
    Wight PAL (1962) The histopathology of the central nervous system in fowl paralysis. J Comp Pathol Ther 72:348–359Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • D. E. Swayne
    • 1
  • O. J. Fletcher
    • 2
  • L. W. Schierman
    • 2
  1. 1.Department of Veterinary Pathology, College of Veterinary MedicineUniversity of GeorgiaAthensUSA
  2. 2.Department of Avian Medicine; College of Veterinary MedicineUniversity of GeorgiaAthensUSA

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