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Acta Neuropathologica

, Volume 62, Issue 4, pp 268–275 | Cite as

Alzheimer neurofibrillary tangles: Monoclonal antibodies to inherent antigen(s)

  • G. P. Wang
  • I. Grundke-Iqbal
  • R. J. Kascsak
  • K. Iqbal
  • H. M. Wisniewski
Original Works

Summary

Using isolated Alzheimer neurofibrillary tangles as the immunogen, nine mouse hybridomas were generated which produced antibodies to the tangles as tested in both tissue sections and isolated neurons from Alzheimer brain. Extraction of isolated neurofibrillary tangles with 2% SDS could not remove the antigen(s) with which these monoclonal antibodies reacted. Immunocytochemical study revealed that each of the monoclonal antibodies reacted with one or more of other tissue antigens in addition to the Alzheimer tangles. However, no reaction with either neurofilaments or microtubules was observed with any one of these antibodies. This is the first demonstration of monoclonal antibodies which have been generated against isolated Alzheimer neurofibrillary tangles; these antibodies react with antigen(s) inherent to the tangles.

Key words

Alzheimer disease Senile dementia Alzheimer neurofibrillary tangles Paired helical filaments Monoclonal antibodies 

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Copyright information

© Springer-Verlag GmbH & Co. KG 1984

Authors and Affiliations

  • G. P. Wang
    • 1
  • I. Grundke-Iqbal
    • 1
  • R. J. Kascsak
    • 1
  • K. Iqbal
    • 1
  • H. M. Wisniewski
    • 1
  1. 1.New York State Institute for Basic Research in Developmental DisabilitiesStaten IslandUSA

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