Acta Neuropathologica

, Volume 22, Issue 1, pp 68–78 | Cite as

Spongioblastomas, astrocytomas and rosenthal fibers. Ultrastructural, tissue culture and enzyme histochemical investigations

  • F. Gullotta
  • E. Fliedner
Original Investigations


Electron microscopic and enzyme histochemical investigations were carried out on a group of spongioblastomas and astrocytomas and their tissue cultures. Both neoplastic cell populations are ultrastructurally identical, but show differences in the late stages of cultivation,viz. in the modality of degeneration. The cytoplasm of “spongioblasts” becomes progressively overloaded with filaments, floccular material and fine granular osmiophilic masses, which leads to cell necrosis. These osmiophilic masses correspond to Rosenthal fibers of light microscopy. In some cells, before Rosenthal fibers appear, the granular ground substance is thickened, particularly close to the Golgi apparatus and the ribosomes are increased in number. At times, some mitochondria show an abnormal dense homogeneous matrix. This peculiar cell degeneration seems to be not the consequence of a simple overproduction of filaments, but a manifold cellular disorder akin to a storage dystrophy.


Public Health Tissue Culture Cell Population Light Microscopy Late Stage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • F. Gullotta
    • 1
  • E. Fliedner
    • 1
  1. 1.Institut für Neuropathologie der Universität BonnBonnGermany

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