Acta Neuropathologica

, Volume 22, Issue 1, pp 68–78 | Cite as

Spongioblastomas, astrocytomas and rosenthal fibers. Ultrastructural, tissue culture and enzyme histochemical investigations

  • F. Gullotta
  • E. Fliedner
Original Investigations

Summary

Electron microscopic and enzyme histochemical investigations were carried out on a group of spongioblastomas and astrocytomas and their tissue cultures. Both neoplastic cell populations are ultrastructurally identical, but show differences in the late stages of cultivation,viz. in the modality of degeneration. The cytoplasm of “spongioblasts” becomes progressively overloaded with filaments, floccular material and fine granular osmiophilic masses, which leads to cell necrosis. These osmiophilic masses correspond to Rosenthal fibers of light microscopy. In some cells, before Rosenthal fibers appear, the granular ground substance is thickened, particularly close to the Golgi apparatus and the ribosomes are increased in number. At times, some mitochondria show an abnormal dense homogeneous matrix. This peculiar cell degeneration seems to be not the consequence of a simple overproduction of filaments, but a manifold cellular disorder akin to a storage dystrophy.

Keywords

Public Health Tissue Culture Cell Population Light Microscopy Late Stage 

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References

  1. Adams, C. W. M.: Neurohistochemistry. Amsterdam-London-New York: Elsevier 1965.Google Scholar
  2. Barka, T., Anderson, P. J.: Histochemistry. New York: Harper and Row 1965.Google Scholar
  3. Cervos Navarro, J.: Microscopia electronica de los tumores del sistema nervioso. 3rd Congr. Nacional de Anatomia Patologica Espanola, Bilbao 1967 (in press).Google Scholar
  4. Duffell, D., Farber, L., Chou, S., Hartmann, J. F.: Electron microscopic observations on astrocytomas. Amer. J. Path.43, 539–554 (1963).Google Scholar
  5. Friede, R. L.: Alexander's disease. Arch. Neurol. (Chic.)11, 414–422 (1964).Google Scholar
  6. Gluszcz, A., Giernat, L., Habryka, K., Alwasiak, J., Lach, B., Papierz, W.: Rosenthal fibers, birefringent gliofibrillary changes and intracellular homogeneous conglomerates in tissue cultures of gliomas. Acta neuropath. (Berl.)17, 54–67 (1971).Google Scholar
  7. Gullotta, F.: La genesi formale delle fibre di Rosenthal. Acta neurol. (Napoli)20, 704–711 (1965).Google Scholar
  8. —, Kreutzberg, G.: Das Gliom des Opticus. Morphologische und histochemische Untersuchungen am Schnittpräparat und der Gewebekultur. Acta neuropath. (Berl.)2, 413–424 (1963).Google Scholar
  9. Hallervorden, J.: Die Markscheidenentwicklung und die Rosenthalschen Fasern. Dtsch. Z. Nervenheilk.181, 547–580 (1961).Google Scholar
  10. Herndon, R. M., Rubinstein, L. J., Freeman, J. M., Mathieson, G.: Light and electron microscopic observations on Rosenthal fibers in Alexander's disease and in multiple sclerosis. J. Neuropath. exp. Neurol.29, 524–551 (1970).Google Scholar
  11. Hossmann, K. A., Wechsler, W.: Zur Feinstruktur menschlicher Spongioblastome. Dtsch. Z. Nervenheilk.187, 327–351 (1965).Google Scholar
  12. Kersting, G.: Die Gewebszüchtung menschlicher Hirngeschwülste. Berlin-Göttingen-Heidelberg: Springer 1961.Google Scholar
  13. —: Tissue culture of human gliomas. In: Progress in neurological surgery, vol. 2, pp. 165–202. Editors: H. Krayenbühl, P. E. Maspes and W. H. Sweet. Basel-New York: Karger 1968.Google Scholar
  14. —: Die Großhirngeschwülste des Kindesalters (eine vergleichende Gewebekulturuntersuchung). Verh. dtsch. Ges. Path., 55. Tag., 311–314 (1971).Google Scholar
  15. Luse, S.: Electron microscopy of brain tumors. In: The biology and treatment of intracranial tumors, pp. 75–102. Editors: W. S. Fields and P. C. Sharkey. Springfield, Ill.: Ch. C. Thomas 1962.Google Scholar
  16. Manuelidis, E. E.: Heterologous transplantation of cerebral and cerebellar astrocytomas. Acta neuropath. (Berl.)20, 160–170 (1972).Google Scholar
  17. Matakas, F.: Einfluß der Kultivation in vitro auf das Enzymmuster intrakranieller Tumoren. Dtsch. Z. Nervenheilk.196, 287–299 (1969).Google Scholar
  18. Mölbert, E.: Die Orthologie und Pathologie der Zelle im elektronenmikroskopischen Bild. In: Handbuch der allgemeinen Pathologie, Bd. II/5, S. 238–465. Red. von F. Büchner. Berlin-Heidelberg-New York: Springer 1968.Google Scholar
  19. Nasu, H., Müller, W.: Enzymhistochemische Untersuchungen an Gliomen. Dtsch. Z. Nervenheilk.186, 67–86 (1964).Google Scholar
  20. Ogasawara, N.: Multiple Sklerose mit Rosenthalschen Fasern. Acta neuropath. (Berl.)5, 61–68 (1965).Google Scholar
  21. Raimondi, A. J., Mullan, S., Evans, P. J.: Human brain tumors: an electron microscopic study. J. Neurosurg.19, 731–752 (1962).Google Scholar
  22. Schiffer, D., Fabiani, A., Vesco, C.: Histochemical study of Rosenthal fibers. With observations about enzyme activities. Psychiat. et Neurol. (Basel)147, 68–80 (1964).Google Scholar
  23. Schlote, W.: Rosenthalsche Fasern und Spongioblasten im Zentralnervensystem. II. Elektronenmikroskopische Untersuchungen. Bedeutung der Rosenthalschen Fasern. Beitr. path. Anat.133, 461–480 (1966).Google Scholar
  24. Schochet, S. S., Lampert, P. W., Earle, K. M.: Alexander's disese. A case report with electron microscopic observations. Neurology (Minneap.)18, 543–549 (1968).Google Scholar
  25. Viale, G. L., Andreussi, L.: Histochemical study of the oxidative activity in tumors of the nervous system. Acta neuropath. (Berl.)4, 538–558 (1965).Google Scholar
  26. Yoshida, N., Ohmaru, I., Sato, T.: Electron microscopic studies on brain tumors. In: Electron microscopy. Vth. Intern. Congr. for Electron Microscopy, Philadelphia 1962. Vol. 2, p. 7. Edited by S. S. Breese, Jr. New York-London: Acad. Press 1962.Google Scholar
  27. Wisniewsky, H., Terry, R. D., Hirano, A.: Neurofibrillary pathology. J. Neuropath. exp. Neurol.29, 163–176 (1970).Google Scholar
  28. Zülch, K. J.: Biologie und Pathologie der Hirngeschwülste. In: Handbuch der Neurochirurgie, Bd. 3. Hrsg.: H. Olivecrona u. W. Tönnis. Berlin-Göttingen-Heidelberg: Springer 1956.Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • F. Gullotta
    • 1
  • E. Fliedner
    • 1
  1. 1.Institut für Neuropathologie der Universität BonnBonnGermany

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