Journal of Comparative Physiology B

, Volume 155, Issue 3, pp 367–371 | Cite as

Species differences in the distribution of adrenal free and sulfoconjugated catecholamines

  • Karoly Racz
  • Otto Kuchel
  • Nguyen T. Buu
Article

Summary

We determined free and sulfoconjugated catecholamines (CA) in adrenals of several species by reverse-phase high performance liquid chromatography (HPLC) with electrochemical detection. The two main adrenal CA, free epinephrine (E) and norepinephrine (NE) from eight species (guinea-pig, rat, dog, mice, bovine, cat, green-monkey and human) were considerably different both in the total amount as well as their relative proportions. Free dopamine (DA) content also differed from species to species but this CA was present in a relatively constant proportion, representing about 1% of the total free CA. Phenolsulfotransferase (PST) activity was present in all of the adrenals. Sulfoconjugated CA, however, were only selectively present: E and NE sulfate were entirely absent but in most of these species DA sulfate was detected in a proportion corresponding to 1–10% of the total (free + sulfoconjugated) DA. The adrenal DA sulfate concentrations did not parallel the adrenal PST activity, indicating that this enzyme can not be considered to be an index of the CA sulfates present in this organ.

Keywords

Sulfate Dopamine Liquid Chromatography High Performance Liquid Chromatography Norepinephrine 

Abbreviations

CA

catecholamines

DA

dopamine

DHBA

dihydroxybenzylamine

E

epinephrine

HPLC

high performance liquid chromatography

NE

norepinephrine

PST

phenolsulfotransferase

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Karoly Racz
    • 1
  • Otto Kuchel
    • 1
  • Nguyen T. Buu
    • 1
  1. 1.Laboratory of the Autonomic Nervous SystemClinical Research Institute of MontrealMontrealCanada

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