Acta Neuropathologica

, Volume 77, Issue 5, pp 489–493 | Cite as

Mitochondria covered with a net of parallel and latticed filaments in nigral neurons of monkeys with experimental parkinsonism

  • H. Nakamura
  • S. Kato
  • J. Tanaka
Regular Papers


Nigral neurons of crab-eating monkeys treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) showed a peculiar configuration occasionally in mitochondria. The outer membrane of mitochondria was covered with a net of fine parallel or latticed filaments, which turned spirally about the long axis of the mitochondria. The filaments were approximately 8 nm in diameter: parallel filaments were arranged at intervals of about 13 nm from center to center; and latticed filaments intersected each other at an angle of almost 135°. When mitochondria were present in groups, the intermitochondrial gap was occasionally filled with the same parallel filaments. The netted mitochondria were frequently associated with intramitochondrial abnormalities such as small flocculent inclusions and disintegrated cristae. Only one or two netted mitochondria were counted in the perikaryon of one section of an injured neuron. They appeared in about one-third of mildly or moderately injured neurons in three of six MPTP-treated monkeys, and not in normal surviving and recovering neurons of treated animals, or in neurons of control animals. We consider the netted mitochondria to be a pathological configuration related to a metabolite of oxidation of MPTP, and to be different from the stubby mitochondria reported in amyotrophic lateral sclerosis (ALS) and a non-ALS case.

Key words

Mitochondria Parkinson disease Substantia nigra 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Monkey 


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  1. 1.
    Buckley IK (1975) Three-dimensional fine structure of cultured cells: possible implications for subcellular motility. Tissue Cell 7:51–72Google Scholar
  2. 2.
    Hirano A, Dembitzer HM, Kurland LT, Zimmerman HM (1968) The fine structure of some intraganglionic alterations. Neurofibrillary tangles, granulovacuolar bodies and “rod-like” structures as seen in Guam amyotrophic lateral sclerosis and parkinsonism-dementia complex. J Neuropathol Exp Neurol 27:167–182Google Scholar
  3. 3.
    Hirano A, Donnenfeld H, Sasaki S, Nakano I (1984) Fine structural observations of neurofilamentous changes in amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 43:461–470Google Scholar
  4. 4.
    Hirano A, Nakano I, Kurland LT, Mulder DW, Holley PW, Saccomanno G (1984) Fine structural study of neurofibrillary changes in a family with amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 43:471–480Google Scholar
  5. 5.
    Kusaka H, Hirano A (1984) Stubby mitochondria (in Japanese). Shinkei Naika (Tokyo) 21:623–624Google Scholar
  6. 6.
    Kusaka H, Hirano A (1985) Fine structure of anterior horns in patients without amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 44:430–438Google Scholar
  7. 7.
    Langston JW, Irwin I, Langston EB, Forno LS (1984) 1-Methyl-4-phenyl-1,2,3,6,-tetrahydropyridinium ion (MPP+): identification of a metabolite of MPTP, a toxin selective to the substantia nigra. Neurosci Lett 48:87–92Google Scholar
  8. 8.
    Nicklas WJ, Vyas I, Heikkila RE (1985) Inhibition of NAD-linked oxidation in brain mitochondria by 1-methyl-4-phenyl-pyridine, a metabolite of the neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Life Sci 36:2503–2508Google Scholar
  9. 9.
    Peña CE (1977) Virus-like particles in amyotrophic lateral sclerosis: electron microscopical study of a case. Ann Neurol 1:290–297Google Scholar
  10. 10.
    Peña CE (1980) Periodic units in the intracristal and envelope spaces of neuronal mitochondria. An artifact due to delayed fixation. Acta Neuropathol (Berl) 51:249–250Google Scholar
  11. 11.
    Porter KR, Anderson KL (1982) The structure of the cytoplasmic matrix preserved by freeze-drying and freeze-substitution. Eur J Cell Biol 29:83–96Google Scholar
  12. 12.
    Porter KR, Tucker JB (1981) The ground substance of the living cell. Sci Am 244:40–51Google Scholar
  13. 13.
    Ramsay RR, Salach JI, Singer TP (1986) Uptake of the neurotoxin 1-methyl-4-phenylpyridine (MPP+) by mitochondria and its relation to the inhibition of mitochondrial oxidation of NAD+-linked substrates by MPP+. Biochem Biophys Res Commun 134:743–748Google Scholar
  14. 14.
    Spacek J, Lieberman AR (1980) Relationships between mitochondrial outer membranes and agranular reticulum in nervous tissue: ultrastructural observations and a new interpretation. J Cell Sci 46:129–147Google Scholar
  15. 15.
    Tanaka J, Nakamura H, Honda S, Takada K, Kato S (1988) Neuropathological study on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine of the crab-eating monkey. Acta Neuropathol 75:370–376Google Scholar
  16. 16.
    Trump BF, McDowell EM, Arstila AU (1980) Cellular reaction to injury. In: Hill RB, LaVia MF (eds) Principle of pathobiology, 3rd edn. Oxford University Press, New York, pp 20–111Google Scholar
  17. 17.
    Wolosewick JJ, Porter KR (1979) Microtrabecular lattice of the cytoplasmic ground substance. J Cell Biol 82:114–139Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • H. Nakamura
    • 1
  • S. Kato
    • 1
  • J. Tanaka
    • 1
  1. 1.Division of Neuropathology, Institute of Neurological SciencesTottori University School of MedicineYonagoJapan

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