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Expression of 3-fucosyl-N-acetyllactosamine on glia cells and its putative role in cell adhesion

Acta Neuropathologica volume 75, pages 173–184 (1987)Cite this article

Summary

Mouse monoclonal antibodies from five clones (MMA, 1G10, VIM C6, MC-1 and Tü9), elicited to human myeloid cells and reactive with the 3-fucosyl-N-acetyllactosamine sequence (also termed X-determinant), cross-react with glia cells from human and rat brain. In brain cell cultures from neonatal rats both astrocytes and oligodendrocytes express the X-determinant during the first period of the culture as shown by immunofluorescence tests. While the astrocytes, which exhibit signs of adhesion, permanently express the X-determinant, the oligoden-drocytes lose the epitope nearly completely during culture. The oligodendrocytes preferentially grow on top of the astrocytic layer. After about 8–10 days of culture, numerous X-determinant-positive astrocytic membranes show oligodendrocyte-shaped negative print images underneath the galactocerebroside-positive oligodendrocytes. At this time, the oligodendrocytes are relatively firmly attached to the astrocytic layer. Since participation of the X-determinant in the compaction of the mouse 8–32 cell stage is being discussed, we conclude from our observations that the X-determinant may also play a role in the astrocyte-astrocyte and astrocyte-oligodendrocyte adhesion. It may represent the functionally active carbohydrate moiety of a heterotypic cell adhesion molecule of glia cells.

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Affiliations

  1. Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrasse 52, D-2000, Hamburg 20, Germany

    B. Niedieck & J. Löhler

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  1. B. Niedieck
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  2. J. Löhler
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Supported by the Gemeinnützige Hertie-Stiftung, Frankfurt/Main. The Heinrich-Pette-Institut is financially supported by Freie und Hansestadt Hamburg and Bundesministerium für Jugend, Familie, Frauen und Gesundheit

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Niedieck, B., Löhler, J. Expression of 3-fucosyl-N-acetyllactosamine on glia cells and its putative role in cell adhesion. Acta Neuropathol 75, 173–184 (1987). https://doi.org/10.1007/BF00687079

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  • DOI: https://doi.org/10.1007/BF00687079

Key words

  • Brain cell culture
  • Glia cells
  • Immunolabeling
  • X-determinant
  • Cell adhesion molecule