Skip to main content

Immunocytochemical study of myelin-associated glycoprotein (MAG), basic protein (BP), and glial fibrillary acidic protein (GFAP) in chronic relapsing experimental allergic encephalomyelitis (EAE)

Summary

Chronic relapsing experimental allergic encephalomyelitis (EAE) lesions that resemble those seen in multiple sclerosis (MS) were produced in young Hartley and strain 13 guinea pigs (Lassmann and Wisniewski 1979). To study distributions of myelin-associated glycoprotein (MAG), myelin basic protein (MBP), and glial fibrillary acidic protein (GFAP) in these lesions, paraffin and semithin epon sections of CNS from eight of these guinea pigs were immunostained with antisera to these proteins according to the peroxidase-antiperoxidase (PAP) method. In lesions with active myelin sheath breakdown, changes in anti-MAG and anti-BP immunoreactivity corresponded closely. Abnormal and/or decreased anti-MAG staining did not extend beyond margins of lesions into surrounding areas containing myelin sheaths stained normally by anti-BP and by histological stains for myelin. GFAP-stained astrocyte processes were more numerous and much larger in more chronic lesions. Anti-MAG and anti-BP both stained regenerating myelin sheaths which were very numerous in both paraflin and epon sections. In the latter, anti-MAG also stained some myelin-forming oligodendroglia. The results are additional evidence suggesting that in chronic relapsing EAE, myelin sheaths are the primary target. Oligodendroglia appear to be relatively unaffected and remyelinate most of the demyelinated axons.

This is a preview of subscription content, access via your institution.

References

  • Alvord EC, Jr (1980) Chronic relapsing experimental allergic encephalomyelitis induced in monkeys with myelin basic protein. J Neuropathol Exp Neurol 39:338

    Google Scholar 

  • Baskin DG, Erlandson SL, Parsons JA (1979) Immunocytochemistry with osmium-fixed tissue. I. Lightmicroscopic localization of growth hormone and prolactin with the unlabeled antibody-enzyme method. J Histochem Cytochem 27:867–872

    Google Scholar 

  • Choi BH, Kim RC (1984) Expression of glial fibrillary acidic protein in immature oligodendroglia. Science 223:407–409

    Google Scholar 

  • Favilla JT, Frail DE, Palkovits CG, Stoner GL, Braun PE, Webster H deF (1984) Myelin-associated glycoprotein (MAG) distribution in human central nervous tissue studied immunocytochemically with monoclonal antibody. J Neuro-immunol 6:19–30

    Google Scholar 

  • Fontana A, Fierz W, Wekerle, H (1984) Astrocytes present myelin basic protein to encephalitogenic T-cell lines. Nature 307:273–276

    Google Scholar 

  • Gendelman HE, Wolinsky JS, Johnson RT, Pressman NJ, Pezeshpour GH, Boisset GF (1984) Measles encephalitis: Lack of evidence of viral invasion of the central nervous system and quantitative study of the nature of demyelination. Ann Neurol 15:353–360

    Google Scholar 

  • Itoyama Y, Sternberger NH, Kies MW, Cohen SR, Richardson EP, Jr, Webster H deF (1980a) Immunocytochemical method to identify myelin basic protein in oligodendroglia and myelin sheaths of the human nervous system. Ann Neurol 7:157–166

    Google Scholar 

  • Itoyama Y, Sternberger NH, Webster H deF, Quarles RH, Cohen SR, Richardson EP, Jr (1980b) Immunocytochemical observations on the distribution of myelin-associated glycoprotein and myelin basic protein in multiple sclerosis lesions. Ann Neurol 7:167–177

    Google Scholar 

  • Itoyama Y, Webster H deF (1982) Immunocytochemical study of myelin-associated glycoprotein (MAG) and basic protein (BP) in acute experimental allergic encephalomyelitis (EAE). J Neuroimmunol 3:351–364

    Google Scholar 

  • Lassmann H (1983) Comparative neuropathology of chronic experimental allergic encephalomyelitis and multiple sclerosis. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Lassmann H, Wisniewski HM (1979) Chronic relapsing experimental allergic encephalomyelitis. Clinicopathological comparison with multiple sclerosis. Arch Neurol 36:490–497

    Google Scholar 

  • McFarlin DE, Blank SE, Kibler RF (1974) Recurrent experimental allergic encephalomyelitis in the Lewis rat. J Immunol 113:712–715

    Google Scholar 

  • Omlin FX, Webster H deF, Palkovits CG, Cohen SR (1982) Immunocytochemical localization of basic protein in major dense line regions of central and peripheral myelin. J Cell Biol 95:242–248

    Google Scholar 

  • Quarles RH, Everly JL, Brady RO (1973) Evidence for the close association of a glycoprotein with myelin in rat brain. J Neurochem 21:1177–1191

    Google Scholar 

  • Quarles RH, Trapp BD (1984) Localization of myelin-associated glycoprotein (Letter to the Editor). J Neurochem 43:1773–1774

    Google Scholar 

  • Raine CS (1983) Multiple sclerosis and chronic relapsing EAE: Comparative ultrastructural neuropathology. In: Hallpike J, Adams CWM, Tourtelotte WW (eds) Multiple sclerosis — pathology, diagnosis and management. Chapman and Hall, London, pp 413–460

    Google Scholar 

  • Raine CS, Barnett LB, Brown A, Behar T, McFarlin DE (1980) Neuropathology of experimental allergic encephalomyelitis in inbred strains of mice. Lab Invest 43:150–157

    Google Scholar 

  • Raine CS, Snyder DH, Valsalmis MP, Stone SH (1974) Chronic experimental allergic encephalomyelitis in inbred guinea pigs — an ultrastructural study. Lab Invest 31:367–380

    Google Scholar 

  • Sato S, Quarles RH, Brady RO (1982) Susceptibility of the myelin-associated glycoprotein and basic protein to a neutral protease in highly purified myelin from human and rat brain. J Neurochem 39:97–105

    Google Scholar 

  • Shii H, Webster H deF, Lassmann H (1982) Distribution of myelin-associated glycoprotein (MAG), myelin basic protein (MBP), and glial fibrillary acidic protein (GFAP) in chronic relapsing experimental allergic encephalomyelitis (EAE). Neurosci Soc Abstr 8:249

    Google Scholar 

  • Smith ME, Somera FP, Eng LF (1983) Immunocytochemical staining for glial fibrillary acidic protein and the metabolism of cytoskeletal proteins in experimental allergic encephalomyelitis. Brain Res 264:241–253

    Google Scholar 

  • Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333

    Google Scholar 

  • Sternberger NH, Itoyama Y, Kies MW, Webster HdeF (1978) Myelin basic protein demonstrated immunocytochemically in oligodendroglia prior to myelin sheath formation. Proc Nat Acad Sci USA 75:2521–2524

    Google Scholar 

  • Sternberger NH, Quarles RH, Itoyama Y, Webster HdeF (1979) Myelin-associated glycoprotein demonstrated immunocytochemically in myelin and myelin-forming cells of developing rat. Proc Nat Acad Sci USA 76:1510–1514

    Google Scholar 

  • Stone SH, Lerner EM (1965) Chronic disseminated allergic encephalomyelitis in guinea pigs. Ann NY Acad Sci 122:227–241

    Google Scholar 

  • Stoner GL (1984) Faulty myelin basic protein phosphorylation could precipitate demyelination. Trans Am Soc Neurochem 15:269

    Google Scholar 

  • Webster H deF (1984) Myelin-associated glycoprotein localization: evidence and interpretations (Letter to the Editor). J Neurochem 43:1774–1777

    Google Scholar 

  • Webster H deF, Palkovits CG, Stoner GL, Favilla JT, Frail DE, Braun PE (1983) Myelin-associated glycoprotein: electron-microscopic immunocytochemical localization in compact developing and adult central nervous system myelin. J Neurochem 41:1469–1479

    Google Scholar 

  • Winchell KH, Sternberger NH, Webster H deF (1982) Myelin-associated glycoprotein localized immunocytochemically in periaxonal regions of oligodendroglia during hexachlorophene intoxication. Brain Res 239:679–684

    Google Scholar 

  • Wisniewski HM, Keith AB (1977) Chronic relapsing experimental allergic encephalomyelitis — an experimental model of multiple sclerosis. Ann Neurol 1:144–148

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Supported in part by the Science Research Fund (Austria), project no.525-07

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Webster, H.d.F., Shii, H. & Lassmann, H. Immunocytochemical study of myelin-associated glycoprotein (MAG), basic protein (BP), and glial fibrillary acidic protein (GFAP) in chronic relapsing experimental allergic encephalomyelitis (EAE). Acta Neuropathol 65, 177–189 (1985). https://doi.org/10.1007/BF00686996

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00686996

Key words

  • Guinea pigs
  • Chronic relapsing experimental allergic encephalomyelitis (EAE)
  • Myelin-associated glycoprotein (MAG)
  • Myelin basic protein (MBP)
  • Glial fibrillary acidic protein (GFAP)