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Acta Neuropathologica

, Volume 15, Issue 3, pp 197–207 | Cite as

Changes in the central nervous system of lambs following the administration of sodium diethyldithiocarbamate

  • J. M. Howell
  • J. Ishmael
  • R. Ewbank
  • W. F. Blakemore
Original Investigations

Summary

Two days old and 1 month old lambs were given repeated intraperitoneal injections of sodium diethyldithiocarbamate (NDDC) in phosphate buffer or phosphate buffer alone. All but one NDDC treated lamb died before 16 weeks when this animal and the 4 controls were killed. Most but not all the treated lambs had elevated copper levels, in liver and spinal cord. Eosinophilic bodies were found in all lambs that had received NDDC for 3 weeks or longer. The light and electronmicroscopic appearance of these bodies is described. The findings are discussed in relation to the close similarity which exists between these changes and those found in naturally occurring neuroaxonal dystrophy of man and sheep and to that found in long-standing vitamin E deficiency in the rat and after the application of certain neurotoxic poisons. A possible explanation for these changes is mentioned.

Key-Words

Lesions in Central Nervous System Lambs Copper Chelating Agent Sodium Diethyldithiocarbamate Neuroaxonal Dystrophy 

Zusammenfassung

Lämmern im Alter von 2 Tagen und 1 Monat wurden wiederholt intraperitoneale Injektionen von Natrium-Diäthyldithiocarbamat (NDDC) in Phosphatpuffer oder Phosphatpuffer allein verabreicht. Mit Ausnahme eines Tieres starben alle mit NDDC behandelten Lämmer innerhalb von 16 Wochen. Ein nicht spontan verendetes Versuchstier und 4 Kontrolltiere wurden zu diesem Zeitpunkt getötet. Die meisten behandelten Tiere zeigten erhöhte Kupferwerte in Leber und Rückenmark. Im ZNS aller Tiere, die NDDC durch 3 Wochen und länger erhalten hatten, fanden sich eosinophile Körperchen, deren licht- und elektronenoptisches Verhalten beschrieben wird. Sie zeigen große Ähnlichkeit mit den Veränderungen bei spontaner neuroaxonaler Dystrophie des Menschen und der Schafe sowie nach chronischem Vitamin E-Mangel der Ratte und nach Verabreichung verschiedener Neurotoxine. Diese Befunde werden diskutiert und ihre mögliche Entstehung erörtert.

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Copyright information

© 1970 1970

Authors and Affiliations

  • J. M. Howell
    • 1
  • J. Ishmael
    • 1
  • R. Ewbank
    • 2
  • W. F. Blakemore
    • 3
  1. 1.Department of Veterinary PathologyUniversity of LiverpoolLiverpoolUK
  2. 2.Sub-Department of Animal HusbandryUniversity of LiverpoolLiverpoolUK
  3. 3.M.R.C. Unit in Applied NeurobiologyInstitute of NeurologyLondon

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