Journal of comparative physiology

, Volume 125, Issue 2, pp 151–156 | Cite as

Developmental changes in the fatty acid composition and cholesterol content of chicken heart plasma membrane

  • Howard Kutchai
  • Thomas F. Ross
  • David M. Dunning
  • Marsha Martin
  • Susan L. King
Article

Summary

  1. 1.

    The fatty acid composition and cholesterol/phospholipid molar ratio of lipids extracted from subcellular fractions of 5–6, 10, and 20 day chick embryo heart and from adult chicken heart were determined.

     
  2. 2.

    The fraction of saturated fatty acids decreases steadily during embryonic and post-hatching development.

     
  3. 3.

    The cholesterol/phospholipid molar ratio decreases between 5–6 and 10 days of embryonic life, increases somewhat between 10 and 20 days of incubation, and decreases again during post-hatching development.

     
  4. 4.

    The pattern of changes in lipid composition is similar in the plasma membrane enriched fraction, the fraction enriched in mitochondria and endoplasmic reticulum, and in the homogenate.

     
  5. 5.

    The changes in the fatty acid composition and cholesterol/phospholipid molar ratio that occur during development are consistent with developmental changes in the microviscosity of the plasma membrane enriched fraction that we previously reported.

     

Keywords

Lipid Cholesterol Endoplasmic Reticulum Fatty Acid Composition Human Physiology 

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References

  1. Barenholz, Y., Moore, N.F., Wagner, R.R.: Enveloped viruses as model membrane systems: microviscosity of vesicular stomatitis virus and host cell membranes. Biochem.15, 3563–3569 (1976)Google Scholar
  2. Bartlett, G.R.: Phosphorus assay in column chromatography. J. biol. Chem.234, 466–468 (1959)Google Scholar
  3. Bligh, E.G., Dyer, W.J.: A rapid method of total lipid extraction and purification. Canad. J. Biochem.37, 911–917 (1959)Google Scholar
  4. Boland, R.A., Martonosi, A., Tillack, T.W.: Developmental changes in the composition and function of sarcoplasmic reticulum. J. biol. Chem.249, 612–623 (1974)Google Scholar
  5. Chapman, D., Penkett, S.A.: Nuclear magnetic resonance studies of the interaction of phospholipids with cholesterol. Nature211, 1304–1305 (1966)Google Scholar
  6. Chapman, D., Wallach, D.F.H.: Recent physical studies of phospholipids and natural membranes. In: Biological membranes —physical fact and function (ed. Chapman, D.), pp. 125–202. London: Academic Press 1968Google Scholar
  7. Courchaine, A.J., Miller, W.H., Stein, D.B.: Rapid semimicro procedure for estimating free and total cholesterol. Clin. Chem.5, 609–614 (1959)Google Scholar
  8. Cronan, J.E., Jr.: Thermal regulation of the membrane lipid composition ofEscherichia coli. Evidence for the direct control of fatty acid synthesis. J. biol. Chem.250, 7074–7077 (1975)Google Scholar
  9. Inbar, M., Shinitzky, M.: Increase of cholesterol level in the surface membrane of lymphoma cells and its inhibitory effect on ascites tumor development. Proc. nat. Acad. Sci. U.S.71, 2128–2130 (1974)Google Scholar
  10. Kent, C., Schimmel, S.D., Vagelos, P.R.: Lipid composition of plasma membranes from developing chick muscle cells in culture. Biochim. biophys. Acta360, 312–321 (1974)Google Scholar
  11. Kidwai, A.M., Radcliffe, M.A., Duchon, G., Daniel, E.E.: Isolation of plasma membrane from cardiac muscle. Biochem. biophys. Res. Commun.45, 901–910 (1971)Google Scholar
  12. Kutchai, H., Barenholz, Y., Ross, T.F., Wermer, D.E.: Developmental changes in plasma membrane fluidity in chick embryo heart. Biochim. biophys. Acta436, 101–112 (1976)Google Scholar
  13. Lenz, B.R., Barenholz, Y., Thompson, T.E.: Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes. Biochem.15, 4521–4528 (1976)Google Scholar
  14. Linden, C.D., Fox, C.F.: Membrane physical state and function. Acets. Chem. Res.10, 321–327 (1975)Google Scholar
  15. Miyamoto, K., Stephanides, L.M., Bernsohn, J.: Fatty acids of glycerophosphatides in developing chick embryonic brain and liver. J. Lipid Res.7, 664–670 (1966)Google Scholar
  16. Oldfield, E., Chapman, D.: Effects of cholesterol and cholesterol derivatives on hydrocarbon chain mobility in lipids. Biochem. biophys. Res. Commun.43, 610–616 (1971)Google Scholar
  17. Overath, P., Schairer, H.U., Stoffel, W.: Correlation of in vivo and in vitro phase transitions of membrane lipids inEscherichia coli. Proc. nat. Acad. Sci. U.S.67, 606–612 (1970)Google Scholar
  18. Rogers, C.G.: Fatty acids and phospholipids of adult and newborn rat hearts and of cultured, beating neonatal rat myocardial cells. Lipids9, 541–547 (1974)Google Scholar
  19. Rottem, S., Cirillo, V.P., DeKruyff, B., Shinitzky, M., Razin, S.: Cholesterol in mycoplasma membranes. Correlation of enzymic and transport activities with physical state of lipids in membranes ofMycoplasma mycoides var.capri adapted to grow with low cholesterol concentrations. Biochim. biophys. Acta323, 509–519 (1973)Google Scholar
  20. Shinitzky, M., Dianoux, A.-C., Gitler, C., Weber, G.: Microviscosity and order in the hydrocarbon region of micelles and membranes determined with fluorescent probes. I. Synthetic micelles. Biochem.10, 2106–2113 (1971)Google Scholar
  21. Shinitzky, M., Inbar, M.: Difference in microviscosity induced by different cholesterol levels in the surface membrane lipid layer of normal lymphocytes and malignant lymphoma cells. J. molec. Biol.85, 603–616 (1974)Google Scholar
  22. Wood, R.: Relationship between embryonic and tumor lipids. I. Changes in the neutral lipids of the developing chick brain, heart, and liver. Lipids7, 596–603 (1972)Google Scholar
  23. Wood, R.: Embryonicvs. tumor lipids. II. Changes in phospholipids of developing chick brain, heart, and liver. Lipids9, 429–439 (1974)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Howard Kutchai
    • 1
  • Thomas F. Ross
    • 1
  • David M. Dunning
    • 1
  • Marsha Martin
    • 1
  • Susan L. King
    • 1
  1. 1.Department of PhysiologyUniversity of Virginia School of MedicineCharlottesvilleUSA

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