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In vitro evaluation of a novel chemotherapeutic agent, adozelesin, in gynecologic-cancer cell lines

  • Original Articles
  • Adozelesin in Cell Lines, Gynecologic Cancer, Adozelesin
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Summary

Adozelesin is a derivative of an extremely cytotoxic compound, CC1065. This entirely new class of drug binds preferentially to DNA and facilitates alkylation reaction. In the present study, we used the adenosine triphosphate (ATP) chemosensitivity assay to compare the cytotoxic potency of Adozelesin with that of common chemotherapeutic agents in ten gynecologic-cancer cell lines. Flow cytometry was also used to study its effects on cell-cycle kinetics. The mean drug concentrations required to produce a 50% reduction in ATP levels as compared with controls [IC50] were: Adriamycin, 0.17±0.06 μm; 4OH-Cytoxan, 18±3 μm; cisplatin, 17±7 μm; 5-fluorouracil, 183±116 μm; and Adozelesin, 11.0±5.4pm. Thus, Adozelesin was 104–107 times more potent than Adriamycin, cisplatin, 5-fluorouracil, and Cytoxan. Cell kinetics studies revealed significant S and G2 blocks such as those previously reported for other alkylating agents.

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Authors and Affiliations

  1. Division of Gynecologic Oncology, Department of Obstetrics & Gynecology, University of Miami School of Medicine, P.O. Box 016 960 (D-52), 33101, Miami, FL, USA

    Hoa N. Nguyen, Bernd-Uwe Sevin, Hervy Averette, James Perras, Randy Hightower, Ronaldo Ramos, Daniel Donato & Mannuel Penalver

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  1. Hoa N. Nguyen
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  2. Bernd-Uwe Sevin
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  3. Hervy Averette
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  4. James Perras
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  5. Randy Hightower
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  6. Ronaldo Ramos
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  7. Daniel Donato
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  8. Mannuel Penalver
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Additional information

Supported in part by an American Cancer Society Clinical Oncology Fellowship and an American Cancer Society/Florida Division Startup Grant (both awarded to H. N. N.)

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Nguyen, H.N., Sevin, BU., Averette, H. et al. In vitro evaluation of a novel chemotherapeutic agent, adozelesin, in gynecologic-cancer cell lines. Cancer Chemother. Pharmacol. 30, 37–42 (1992). https://doi.org/10.1007/BF00686483

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  • DOI: https://doi.org/10.1007/BF00686483

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