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Dominant expression of multidrug resistance in intraspecific murine lymphoma hybrid cells

  • Short Communication
  • Antitumor Drugs, Intraspecific Lymphoma Hybrids, Multidrug Resistance Expression
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Abstract

Cultured P388/VCR mouse lymphoma cells resistant to vincristine (VCR) and to 5-bromodeoxyuridine (BUdR) and deficient in thymidine kinase (TK-) were fused with P388/DAG cells resistant to 1,2:5,6-dianhydrogalactitol (DAG), an anticancer alkylating agent, and to 6-thioguanine (6-TG) and deficient in hypoxanthine phosphoribosyl-transferase (HPRT-). The hybrid cells expressed multidrug resistance (MDR), i.e., resistance to VCR and cross-resistance to Adriamycin (ADM) and actinomycin D (Act. D), in a dominant manner. The presence of glycoprotein p170, the MDR gene product, was detected in the hybrid cells. Resistance to DAG was also expressed dominantly, whereas cross-resistance to dibromodulcitol (DBD), a chemically related anticancer drug, was slight.

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Authors and Affiliations

  1. Research Center of Oncology, National Institute of Oncology, P.O.B. 21, 1525, Budapest 114, Hungary

    István Pályi, Gizella Turi, Lehel Hullán & Mihály Bak

  2. 1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary

    Károly Szikla

Authors
  1. István Pályi
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  2. Gizella Turi
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  3. Lehel Hullán
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  4. Károly Szikla
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  5. Mihály Bak
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Pályi, I., Turi, G., Hullán, L. et al. Dominant expression of multidrug resistance in intraspecific murine lymphoma hybrid cells. Cancer Chemother. Pharmacol. 34, 81–85 (1994). https://doi.org/10.1007/BF00686118

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  • DOI: https://doi.org/10.1007/BF00686118

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