Journal of Comparative Physiology B

, Volume 155, Issue 2, pp 135–143 | Cite as

Isolation and characterization of plasma coagulogen (PC) of the cockroachLeucophaea maderae (Blattaria)

  • Barbara Barwig


  1. 1.

    The plasma coagulogen (PC), one of the two clotting proteins of the African cockroachLeucophaea maderae, was obtained from the hemolymph of last nymphal instars, isolated and further analyzed.

  2. 2.

    The isolation of plasma coagulogen was carried out by two methods, (i) dissociation of plasma coagulogen from an association of plasma coagulogen and insoluble hemocyte coagulogen, and (ii) precipitation with buffer of low ionic strength.

  3. 3.

    The purified protein was homogeneous when tested by polyacrylamide gel electrophoresis, immunoelectrophoresis and sedimentation analysis.

  4. 4.

    PC is a lipoprotein with a lipid content of 50%. A carbohydrate component of about 1% was also detected. The molecular weight of the lipoprotein is about 640,000. The sedimentation coefficient was determined to be 7.7 S.

  5. 5.

    SDS-PAGE of plasma coagulogen revealed two subunits: a heavy chain (PC1) with a molecular weight of about 320,000 and a light chain (PC2), with 76,000.

  6. 6.

    The amino acid analysis indicated high glutamine/glutamic acid as well as asparagine/aspartic acid values.

  7. 7.

    The lipid component showed a high percentage of phospholipids and diglycerides.

  8. 8.

    PC is suggested to be identical with lipophorin because of its high similarity with other insect lipophorins.



Lipid Carbohydrate Polyacrylamide Ionic Strength Lipid Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





ethylenediamine-tetraacetic acid, disodium salt


hemocyte coagulogen


polyacrylamide gel electrophoresis


periodic acid-Schiff reagent


plasma coagulogen


sodium dodecyl sulfate


thin layer chromatography


thin layer gel filtration

\(\bar v\)

partial specific volume


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Barbara Barwig
    • 1
  1. 1.Zoologisches Institut der UniversitätMünchen 2Federal Republic of Germany

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