Acta Neuropathologica

, Volume 43, Issue 1–2, pp 111–117 | Cite as

Tumor specific fluorescent and complement-dependent cytotoxic antibodies in the serum of rats with chemically induced brain gliomas

  • D. Stavrou
  • A. P. Anzil
  • H. Elling
Original Investigations


Brain tumors were induced in Sprague-Dawley and Long-Evans rats by administration of N-methyl-N-nitrosourea in the drinking water. Of these tumors, a grade 2 mixed glioma, a grade 2 to 3 astrocytoma and a grade 1 to 2 oligodendroglioma were established in vitro, maintained in culture and designated 75SD-G-376, 75SD-G-420 and 77LE-G-180, respectively. Of these mass cultures, two were successfully cloned and are currently available as 75SD-G-376C and 75SD-420C cell lines. Clonal lines produce S-100 protein and grow as tumors when isografted in young rats.

Using the cultured cells as target cells, specific antibodies were searched for in the sera of the rats with the primary tumors by means of an indirect fluorescent antibody staining method and a complement-dependent antibody-mediated microcytotoxicity assay. Fluorescent and cytotoxic antibodies were demonstrated in the sera of the mixed glioma- and astrocytoma-bearing animals. However, a variable proportion of cells of the 75SD-G-376 and 75SD-G-420 lines showed no reaction with the corresponding sera. Furthermore, cytotoxic anfibodies had a lytic effect on the autologous glioma cells only in the presence of rabbit complement.

Key words

Experimental brain gliomas Chemically induced Rat Cell cultures Humoral antibodies Immunofluorescence staining Microcytotoxicity assay 


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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • D. Stavrou
    • 1
  • A. P. Anzil
    • 2
  • H. Elling
    • 1
  1. 1.Lehrstuhl für Allgemeine Pathologie und Neuropathologie am Institut für Tierpathologie der Ludwig-Maximilians-Universität MünchenMünchen 22Federal Republic of Germany
  2. 2.Max-Planck-Institut für PsychiatrieMünchen 40Federal Republic of Germany

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