Acta Neuropathologica

, Volume 24, Issue 2, pp 107–116 | Cite as

Tissue culture, electron microscopic and enzyme histochemical investigations on a sympathetic ganglioneuroblastoma

  • F. Gullotta
  • E. Fliedner
  • R. Wüllenweber
  • G. Orf
Original Investigations


The results of combined tissue culture, ultrastructural and enzyme histochemical investigations carried out on a sympathetic ganglioneuroblastoma are reported.In vitro a vigorous sprouting of newly formed neuritic processes was observed a few days after explantation. The enzymatic reactions for acethylcholinesterase were positivein situ as well asin vitro. The tumor elements showed the ultrastructural characteristics of nerve cells with many microtubuli and filaments, plenty of ribosomes, well developed endoplasmic reticulum. Dense bodies, corresponding to neurosecretion granula (catecholamines) were also observed. Synaptic structures were missing. The results of these investigations confirm that this kind of tumor is different from so-called medulloblastoma.

Key words

Tissue Culture Electron Microscopy Enzyme Histochemistry Sympathetic Ganglioneuroblastoma Medulloblastoma 


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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • F. Gullotta
    • 1
  • E. Fliedner
    • 1
  • R. Wüllenweber
    • 1
  • G. Orf
    • 1
  1. 1.Institut für Neuropathologie, Neurochirurgische Klinik der Universität BonnBonn-VenusbergFederal Republic of Germany

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