Journal of Comparative Physiology B

, Volume 155, Issue 4, pp 493–499 | Cite as

Identification of crystalline allantoin in the urine of African Cricetidae (Rodentia) and its role in their water economy

  • Rochelle Buffenstein
  • W. E. Campbell
  • J. U. M. Jarvis
Article

Summary

All eleven cricetid species, examined in this investigation, produced an off-white crystal-line precipitate in their urine when deprived of water, whereas not one murid examined did so. This crystalline compound was identified as allantoin, a common end product of purine catabolism. The quantity found in the solid precipitate alone accounted for 47% of the total nitrogen excreted and was approximately 14 times greater than the predicted quantity of allantoin from purine degradation. It appears that there is a shift in nitrogen excretion from urea to allantoin in the Cricetidae.

Water-deprived cricetids had higher urine osmolalities, urea concentrations and lower daily percentage body water turnovers than the murids. This can be explained by the substantial water savings associated with excreting solid allantoin. The discrepancy in the mode of nitrogen excretion between the two families inhabiting the Namib Desert can be attributed to their different evolutionary histories, the Cricetidae being pre-adapted for survival in deserts.

Keywords

Total Nitrogen Purine Urea Concentration Water Saving Urine Osmolality 

Abbreviations

WTR

water turnover rate

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References

  1. Abraham J, Simeone FA, Hopkins FA (1976) A sensitive assay for allantoin. Anal Biochem 70:377–380Google Scholar
  2. Barash J (1977) Accumulation of urea and allantoin during purine utilization by germinating spores ofGeothrichum candidum. J Gen Microbiol, 72:539–542Google Scholar
  3. Borchers R (1977) Allantoin determination. Anal Biochem 79:612–613Google Scholar
  4. Borut A, Shkolnik A (1974) Physiological adaptation to the desert environment. In: Robertshaw D (ed) Environmental physiology. International Review of Science, Physiology, Ser. 7. Butterworths, London MTP, pp 185–229Google Scholar
  5. Buffenstein R (1977) Adaptive renal efficiency in three species of rodent. Unpublished honours project, University of Cape TownGoogle Scholar
  6. Buffenstein R (1984) Energy and water balance during torpor and hydropenia in the pigmy gerbil,Gerbillus pusillus. J Comp Physiol B 154:535–544Google Scholar
  7. Buffenstein R (1985) The effect of a high fibre diet on energy and water balance in two Namib desert rodents. J Comp Physiol, B 155:211–218Google Scholar
  8. Byers SO, friedman M, Garfield MM (1947) The blood uric acid and allantoin of the rat after nephrectomy and hepatectomy. Am J Physiol 15:677–681Google Scholar
  9. Chaney AL, Marbach EP (1962) Modified reagents for determination of urea and ammonia. Clin Chem 8:131–135Google Scholar
  10. Christian DP (1979) Physiological correlates of demographic patterns in three sympatric Namib desert rodents. Physiol Zool 52:329–339Google Scholar
  11. Coetzee CG (1969) The distribution of mammals in the Namib desert and adjoining inland escarpment. Sci Papers Namib Desert Res Station 40:23–36Google Scholar
  12. Drilhon A, Marcoux F (1942) Etude biochimique du sang et de l'urine d'un chélonien:Testudo mauritanica. Bull Soc Chim Biol Paris 24:103–107Google Scholar
  13. Ghobrial LI, Nour TA (1975) The physiological adaptations of desert rodents. In: Prakash I, Ghosh PK (eds) Rodents in desert environments. Junk, The Hague, Monographiae Biol 28:413–414Google Scholar
  14. Greger R, Lang F, Deetjen P (1975) Handling of allantoin by the rat kidney. Clearance and micropuncture data. Pflügers Arch 357:201–207Google Scholar
  15. Greger R, Lang F, Deetjen P (1976) Renal excretion of purine metabolites, urate and allantoin, by the mammalian kidney. Int Rev Physiol Kidney Urinary Tract Physiol 11:257–281Google Scholar
  16. Halpern BH, Pacaud A (1951) Technique de prélèvement d'echantillons de sang chez les petits animaux de laboratoire par fonction du plexus ophthalmique. CR Séanc Soc Biol 145:1465–1466Google Scholar
  17. Hewitt S, Wheldrake JF, Baudinette RV (1981) Water balance and renal function in the Australian, desert rodentNotomys alexis: the effect of diet on water turnover rate, glomerular filtration rate, renal plasma flow and renal blood flow. Comp Biochem Physiol 68A:405–440Google Scholar
  18. Holleman DF, Dietrich RA (1973) Body water content and turnover in several species of rodents as calculated by the tritiated water method. J Mammal 54:456–465Google Scholar
  19. Kageyama N (1971) A direct colorimetric determination of uric acid in serum and urine with uricase-catalase system. Clin Chim Acta 31:421–426Google Scholar
  20. Kiriyama S, Ashida K (1964) Effect of the quality of dietary protein on nitrogen compounds in the urine of rats. J Nutr 82:127–134Google Scholar
  21. Larson HW (1931) A colorimetric method for the determination of allantoin. J Biol Chem 94:727–738Google Scholar
  22. Lehninger A (1970) Biochemistry. Worth Publishers, New YorkGoogle Scholar
  23. Loveridge JP (1970) Observations on nitrogenous excretion and water relations ofChiromantis xerampelina (Amphibia, Anura). Arnoldia 5:1–6Google Scholar
  24. MacMillen RE, Baudinette RV, Lee AK (1972) Water economy and energy metabolism of the sandy inland mouse,Leggadina hermansburgensis. J Mammal 53:529–539Google Scholar
  25. MacMillen RE, Lee AK (1969) Water metabolism of Australian hopping mice. Comp Biochem Physiol 28:493–514Google Scholar
  26. Mares MA, Blair WF, Enders FA, Greegor D, Hulse AC, Hunt JH, Otte D, Sage RD, Tomoff CS (1977) The strategies and community patterns of desert animals. In: Orians GH, Solbrig OT (eds) Convergent evolution in warm deserts. Dowden Hutchinson and Ross, Cambridge Massachusetts, pp 107–163Google Scholar
  27. Meester J (1965) The origins of the southern African mammal fauna. Zool Afr 1:87–93Google Scholar
  28. Missone X (1969) African and Indo-Australian Muridae. Evolutionary trends. Tervuren, Belgique, Ann, Musée Royal Afrique centrale, Sér Sci Zool 172:1–219Google Scholar
  29. Missone X (1974) Rodentia, Part 6. In: Meester J, Setzer HW (eds) The mammals of Africar. an identification manual. Smithsonian University Press, Washington, pp 1–39Google Scholar
  30. Morgan EH, Hanson A (1964) Serum and urine allantoin in pregnancy and lactation in the rat. Acta Physiol Scand 60:164–169Google Scholar
  31. Pak N, Donoso G, Tagle MA (1973) Allantoin excretion in the rat. Br J Nutr 30:107–112Google Scholar
  32. Richmond CR, Langham WH, Trujillo TT (1962) Comparative metabolism of tritiated water by mammals. J Cell Comp Physiol 59:45–53Google Scholar
  33. Schmidt-Nielsen K (1964) Desert animals: physiological problems of heat and water. Clarendon Press. OxfordGoogle Scholar
  34. Schmidt-Nielsen K (1979) Animal physiology: adaptation and environment, 2nd edn. Cambridge University Press, CambridgeGoogle Scholar
  35. Tracey MV (1955) Urea and ureids. In: Paech K, Tracey MV (eds) Modern methods of plant analysis, vol 4. Springer, Berlin Göttingen Heidelberg, pp 119–141Google Scholar
  36. Vrbaski MM, Grujic'-Injac B, Gajic' D (1978) A new method for allantoin determination and its application in allantoin determination inAgrostemma githago L seeds. Anal Biochem 91:304–308Google Scholar
  37. Withers PC (1979) Ecology of a small mammal community on a rocky outcrop in the Namib desert. Madoqua 2:229–246Google Scholar
  38. Yousef MK, Johnson HD, Bradley WG, Seif SM (1974) Tritiated water turnover rate in rodents: desert and mountain. Physiol Zool 47:153–162Google Scholar
  39. Young EG, Conway CF (1942) On the estimation of allantoin by the Rimini-Schryver reaction. J Biol Chem 142:839–852Google Scholar
  40. Zar JH (1974) Biostatistical analysis. Prentice Hall, New JerseyGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Rochelle Buffenstein
    • 1
  • W. E. Campbell
    • 2
  • J. U. M. Jarvis
    • 1
  1. 1.Department of ZoologyUniversity of Cape TownRondebosch
  2. 2.Department of ChemistryUniversity of Cape TownRondebosch

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