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Acta Neuropathologica

, Volume 22, Issue 3, pp 245–256 | Cite as

Désafférentation expérimentale de l'écorce cérébrale: Étude par la méthode de Golgi-Cox en microscopies optique et électronique

  • Claude Fuentes
  • Robert Marty
Travaux Originaux

Cortical deafferentation: A light and electron microscopy study by the Golgi-Cox method

Summary

The auditive cortical area of the cat, which had been deafferentated from the medial geniculate body by electrolytic lesion and impregnated by the Golgi-Cox method, was studied by light and electron microscopy at various intervals after the intervention and compared with normal animals. In light microscopy the gradual breakdown of the dendrites, followed by that of cellular body of the majority of the impregnated neurons was observed. On the 25th day after the intervention the only visible cellular elements were neuroglial. In the thin sections the impregnated neurons revealed similar modifications to those mentioned above and coexisted with the unimpregnated neurons and the neuroglial elements. In electron microscopy, the impregnated neurons appeared dark and homogenous in the normal animal. The disappearance of the endoplasmic organisation contrasted with the conservation of the nucleus. After deafferentation the cytoplasm revealed an ever increasing loss of substance in the form of rounded shapes corresponding approximately to the size of the metallic deposits.

On the basis of previous results, where the deafferentation was studied by electron microscopy without metallic impregnation, these results can be interpreted as expressing, on the part of the deafferented neurons, the gradual loss of affinity for the mercury. However this fact does not exclude the possibility of a certain number of neurons, already impregnated by the mercury actually desintegrating after the first 3 weeks following the intervention.

Key words

Cat Cerebral Cortex Deafferentation Golgi-Cox Light and Electron Microscopy 

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Claude Fuentes
    • 1
  • Robert Marty
    • 1
  1. 1.Laboratoire de NeurophysiologieUniversité des Sciences et Techniques du LanguedocMontpellierFrance

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