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Distribution of dopamine-beta-hydroxylase—like immunoreactivity in the rat pineal organ

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Summary

The aim of the present investigation was to study the distribution in the rat pineal gland of dopamine-β-hydroxylase (DBH) which is essential for the formation of the melatonin synthesis-regulating substance noradrenaline (NA). In 5- and 8-month-old male Sprague-Dawley rats DBH-like immunoreactivity (DBH-LI) was studied using polyclonal antibodies against DBH and the indirect immunofluorescent technique. DBH-LI was mainly located in pincal nerve fibres coming from the superior cervical ganglia. The intensity of the staining reaction was considerably lower than in non-pineal noradrenergic nerve fibres and the impression was gained by comparison of DBH-LI specimens with glyoxylic acid-treated sections that only approximately one third of the NA-containing intrapineal nerve fibres exhibited DBH-LI. There were no detectable differences in DBH-LI with regard to time of day and age of the animals. These results sugest that NA synthesis may be relatively low in intrapineal sympathetic nerve fibers and that the NA required for the regulation of pineal melatonin synthesis may, to a large degree, stem from the circulation. In addition to nerve fibres, some rare intrapineal cell bodies exhibited DBH-LI; in 5-month-old rats their numbers did not reveal significant differences between day and night. These cells do not appear to represent pinealocytes. They may be a special population of noradrenergic nerve cells perhaps belonging to an as yet unknown intrapineal regulatory system.

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References

  • Ariens Kappers J (1960) The development, topographical relations and innervation of the epiphysis cerebri in the albino rat. Z Zellforsch Mikrosk Anat 52:163–215

    Article  Google Scholar 

  • Bowers CW, Dahm LM, Zigmond RE (1984) The number and distribution of sympathetic neurons that innervate the rat pineal gland. Neuroscience 13:87–96

    Article  PubMed  CAS  Google Scholar 

  • Brimijoin S (1974) Local changes in subcellular distribution of dopamine-beta-hydroxylase (EC 1.14.2.1) after blokade of axonal transport. J Neurochem 22:347–353

    Article  PubMed  CAS  Google Scholar 

  • Brownstein M, Axelrod J (1974) Pineal gland: 24-hour rhythm in norepinephrine turnover. Science 184:163–165

    Article  PubMed  CAS  Google Scholar 

  • Budd GC, Salpeter MM (1968) 3H-norepinephrine-labeled axons in electron microscope radioautograms of pineal body and adrenal capsule. J Cell Biol 39: 19a

    Google Scholar 

  • Budd GC, Salpeter MM (1969) The distribution of labeled norepinephrine within sympathetic nerve terminals studied with electron microscope radioautography. J Cell Biol 41:21–32

    Article  CAS  Google Scholar 

  • Craft M, Morgan WW, Reiter RJ (1984) 24-hour changes in the catecholamine synthesis in rat and hamster pineal glands. Neuroendocrinology 38:193–198

    Article  PubMed  CAS  Google Scholar 

  • David GFX, Umberkoman B, Kumar K, Anand Kumar TC (1975) Neuroendocrine significance of pineal. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction II. The ventricular system in neuroendocrine mechanisms. Proceeding of the Second International Symposium on brain-endocrine function. Shizuola, Japan, October 16–18, 1974. Karger, Basel, pp 365–375

    Google Scholar 

  • Deguchi T, Axelrod J (1973) Supersensitivity and subsensitivity of the β-adrenergic receptor in pineal gland regulated by catecholamine transmitter. Proc Natl Acad Sci USA 70:2208–2211

    Article  Google Scholar 

  • Falck B, Owman C, Rosengren E (1966) Changes in rat pineal stores of 5-hydroxytryptamine after inhibition of its synthesis or break-down. Acta Physiol Scand 67:300–305

    PubMed  CAS  Google Scholar 

  • Håkanson R, Owman Ch (1965) Effect of denervation and enzyme inhibition on DOPA decarboxylase and monoamine oxidase activities of rat pineal gland. J Neurochem 12:417–429

    Article  Google Scholar 

  • Hartman BK (1973) Immunofluorescence of dopamine-beta-hydroxylase. Application of improved methodology to the localization of the peripheral and central noradrenergic system. J Histochem Cytochem 21:321–331

    Google Scholar 

  • Helle KB, Bjerrum OJ, Bock E (1982) Immunochemical characterization of dopamine-beta-hydroxylase. Scand J Immunol [Suppl] 9:97–123

    Article  CAS  Google Scholar 

  • Hökfelt T, Fuxe K, Goldstein M, Joh TH (1973) Immunohistochemical localization of three catecholamine synthesizing enzymes: Aspects on methodology. Histochemie 33:231–254

    PubMed  Google Scholar 

  • König R, Heym Ch (1978) Immunofluorescent localization of dopamine-beta-hydroxylase in small intensely fluorescent cells of the rat superior cervical ganglion. Neurosci Lett 10:187–191

    Article  PubMed  Google Scholar 

  • Korf HW, Møller M (1984) The innervation of the mammalian pineal gland with special reference to central pinealopetal projections. Pineal Res Rev 2:41–86

    Google Scholar 

  • Livett BG, Geffen LB, Austin L (1968) Proximo-distal transport of (14C)-noradrenaline and protein in sympathetic nerves. J Neurochem 15:931–939

    Article  PubMed  CAS  Google Scholar 

  • Matsushima S, Reiter RJ (1977) Fine structural features of adrenergic nerve fibres and endings in the pineal gland of the rat, ground squirrel and chinchilla. Am J Anat 148:463–478

    Article  PubMed  CAS  Google Scholar 

  • McGeer EG, McGeer PL (1966) Circadian rhythm in pineal tyrosine hydroxylase. Science 153:73–74

    Article  PubMed  CAS  Google Scholar 

  • Moore RY, Klein DC (1974) Visual pathways and the central neural control of circadian rhythm in pineal serotonin-N-acetyl-transferase. Brain Res 71:17–33

    Article  PubMed  CAS  Google Scholar 

  • Okuno S, Fujisawa H (1984) Purification and characterization of the rat dopamine-beta-monooxygenase and monoclonal antibodies to the enzyme. Biochim Biophys Acta 799:260–269

    PubMed  CAS  Google Scholar 

  • Owman Ch (1964) Sympathetic nerves probably storing two types of monoamines in the rat pincal gland. Int J Neuropharmacol 3:105–112

    Article  PubMed  CAS  Google Scholar 

  • Parfitt AG, Klein DC (1976) Sympathetic nerve endings in pineal gland protect against acute stress-induced increase in N-acetyltransferase (EC 2.3.1.5) activity. Endocrinology 99:840–851

    PubMed  CAS  Google Scholar 

  • Pellegrino de Iraldi A, Zieher LM (1966) Noradrenaline and dopamine content of normal, decentralized and denervated pineal gland of the rat. Life Sci 5:149–154

    Article  PubMed  CAS  Google Scholar 

  • Quay WB (1974) Pineal chemistry. Charles C. Thomas, Springfield, IL, USA

    Google Scholar 

  • Reuss S, Semm P, Vollrath L (1985) Changes in electrical activity of the rat pineal gland following stimulation of the cervical sympathetic ganglia. J Auton Nerv Syst 12:281–288

    Article  PubMed  CAS  Google Scholar 

  • Schröder H, Gerold N, Heym Ch (1981) Immunofluorescence of dopamine-beta-hydroxylase in small intensely fluorescent cells of human sympathetic ganglia. Acta Anat 109:47–50

    Article  PubMed  Google Scholar 

  • Sternberger L (1979) Immunocytochemistry. John Wiley, New York

    Google Scholar 

  • Thomas JA, Sakai KK, Holck MI, Marks BH (1980) Dopaminebeta-hydroxylase: A modulator of beta adrenergic receptor activity. Res Commun Chem Pathol Pharmacol 29:3–13

    PubMed  CAS  Google Scholar 

  • Torre De la JC, Srugeon JW (1976) A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method. Histochemistry 49:81–93

    Article  PubMed  CAS  Google Scholar 

  • Williams TH, Black jr AC, Chiba T, Jew J (1976) Interneurons/SIF cells in sympathetic ganglia of various mammals. In: Coupland RE, Fujita T (eds) Chromaffin, enterochromaffin and related cells. Elsevier, Amsterdam, pp 95–116

    Google Scholar 

  • Wolfe DE, Potter LE, Richardson KE, Axelrod J (1962) Localizing tritiated norepinephrine in sympathetic axons by electron microscopic autoradiography. Science 138:440–442

    Article  PubMed  CAS  Google Scholar 

  • Wurtman, RJ, Axelrod J (1966) 24-hour rhythm in the content of norepinephrine in the pineal and salivary glands of the rat. Life Sci 5:655–669

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Johannes Gutenberg-University Mainz, Saarstrasse 19/21, D-6500, Mainz, Federal Republic of Germany

    H. Schröder & L. Vollrath

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  1. H. Schröder
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  2. L. Vollrath
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Supported by the Deutsche Forschungsgemeinschaft (DFG) (Grant Schr 283/1-1)

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Schröder, H., Vollrath, L. Distribution of dopamine-beta-hydroxylase—like immunoreactivity in the rat pineal organ. Histochemistry 83, 375–380 (1985). https://doi.org/10.1007/BF00684385

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