Histochemistry

, Volume 83, Issue 3–4, pp 307–313 | Cite as

Ultrastructural cytochemistry of glycoconjugates in basophils from humans and animals

  • H. Sakakibara
  • M. Eguchi
Article

Summary

The distribution of glycoconjugates in the basophil granules of humans, guinea pigs, and rabbits was compared. The observation of acid mucopolysaccharides using the dialyzed iron method and of sulfated glycoconjugates using the high iron diamine method revealed three types of reactions in the basophil granules of all three species: (1) granules showing a strong overall reaction, (2) granules showing reaction only at their periphery, and (3) granules showing no reaction. With regard to the relationship between maturation and the types of basophil granules, it appeared that, in general, there were many type-1 granules among immature basophils, but that these granules decreased in mature basophils as type-3 granules increased. The reaction patterns of periodate-reactive neutral glycoconjugates, as shown by the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method, were different from those of acid mucopolysaccharides: the reaction of basophil granules was diffusely positive, and localization at the periphery was rarely observed. Therefore, unlike the acid mucopolysaccharides, it was difficult to classify the glycoconjugates into three types. However, as with acid mucopolysaccharides, there was a tendency for periodate-reactive glycoconjugates to decrease as maturation progressed. In terms of different species of animals, the reaction of periodate-reactive glycoconjugates with PA-TCH-SP was stronger in humans and rabbits than in guinea pigs.

Keywords

Acid Mucopolysaccharide Glycogen Particle Thiocarbohydrazide Basophil Granule Ultrastructural Cytochemistry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ackerman GA (1963) Cytochemical properties of the blood basophilic granulocyte. Ann NY Acad Sci 103:376–393PubMedCrossRefGoogle Scholar
  2. Avila JL (1978) The influence of the type of sulphate bond and degree of sulphation of glycosaminoglycans on their interactin with lysosomal enzyme. Biochem J 171:489–491PubMedGoogle Scholar
  3. Avila JL, Convit J (1976) Physicochemical characteristics of the glycosaminoglycan-lysosomal enzyme interaction in vitro. A model of control of leukocytic lysosomal activity. Biochem J 160:129–136PubMedGoogle Scholar
  4. Chan BST, Yoffey JM (1960) The basophil cell of guinea-pig bone marrow and their response to horse serum. Immunology 3:237–243PubMedGoogle Scholar
  5. Dunn WB, Spicer SS (1969) Histochemical demonstration of sulfated mucosubstances and cationic proteins in human granulocyte and platelets. J histochem Cytochem 17:668–674PubMedGoogle Scholar
  6. Hardin JH, Spicer SS (1971) Ultrastructural localization of dialysed iron-reactive mucosubstance in rabbit heterophils, basophils and eosinophils. J Cell Biol 48:368–386CrossRefGoogle Scholar
  7. Horn RG, Spicer SS (1964) Sulfated mucopolysaccharide and basic protein in certain granules of rabbit leukocytes. Lab Invest 13:1–15PubMedGoogle Scholar
  8. Jewell GG, Saxton CA (1970) The ultrastructural demonstration of compounds containing 1,2-glycol groups in plant cell walls. Histochem J 2:17–28PubMedCrossRefGoogle Scholar
  9. Komiyama A, Spicer SS (1974) Ultrastructural localization of a characteristic acid phosphatase in granules of rabbit basophils. J Histochem Cytochem 22:1092–1104PubMedGoogle Scholar
  10. Murata F, Nagata T (1976) Fine structure and acid mucosubstance localization of the human basophilic leukocytes. J Clin Electron Microsc 9:207–214Google Scholar
  11. Murata F, Momose Y, Nagata T (1977) Demonstration of intracytoplasmic glycogen of megakaryocytes and blood platelets by means of the periodic acid-thiocarbohydrazide-silver proteinate method. Histochemistry 52:307–316PubMedCrossRefGoogle Scholar
  12. Murata F, Yoshida K, Ohno S, Nagata T (1978) Ultrastructural localization of glycogen in the granulocytes of normal rabbit bone marrow. Histochemistry 58:103–111PubMedCrossRefGoogle Scholar
  13. Olsson I (1969) The intracellular transport of glycosaminoglycans (mucopolysaccharides) in human leukocytes. Exp Cell Res 54:318–324PubMedCrossRefGoogle Scholar
  14. Parmley RT, Ogawa M, Spicer SS, Wright NJ (1976) Ultrastructure and cytochemistry of bone marrow granulocytes in culture. Exp Hematol 4:75–89PubMedGoogle Scholar
  15. Parmley RT, Spicer SS, O'Dell RF (1978) Ultrastructural identification of acid complex carbohydrates in cytoplasmic granules of normal and leukemic human monocytes. Br J Haemtol 39:33–39CrossRefGoogle Scholar
  16. Parmley RT, Spicer SS, Austin RT (1979) Radiosulfate labelling and high iron diamine staining of rabbit granulocytes (abstr). J Histochem Cytochem 27:716Google Scholar
  17. Parmley RT, Spicer SS, Komiyama A, Dow LW, Austin RL (1979) Ultrastructural cytochemistry of chronic myelocytic leukemia. Exp Mol Pathol 30:41–54PubMedCrossRefGoogle Scholar
  18. Parmley RT, Eguchi M, Spicer SS, Alavarez CJ, Austin RL (1980) Ultrastructural cytochemistry and radioautography of complex carbohydrates in heterophil granulocytes from rabbit bone marrow. J Histochem Cytochem 28:1067–1080PubMedGoogle Scholar
  19. Parmley RT, Takagi M, Spicer SS, Thrasher A, Denyo FR (1982) Ultrastructural visualization of complex carbohydrates in eosinophilic leukocytes. Am J Anat 165:53–67PubMedCrossRefGoogle Scholar
  20. Parwaresch MR (1976) The human blood basophil. Springer, Berlin Heidelberg New York, pp 31–41Google Scholar
  21. Rinehart JF, Abul-Haj SK (1951) An improved method for histologic demonstration of acid mucopolysaccharides in tissues. AMA Arch Pathol 52:189–194PubMedGoogle Scholar
  22. Sannes PL, Spicer SS, Katsuyama T (1979) Ultrastructural localization of sulfated complex carbohydrates with a modified iron diamine procedure. J Histochem 27:1108–1111Google Scholar
  23. Schrevel J, Gros D, Monsigny M (1981) Cytochemistry of cell glycoconjugates. Prog Histochem Cytochem 14:1–269PubMedGoogle Scholar
  24. Seligman AM, Hanker JS, Wasserking H, Dmochowski H, Katzoff L (1965) Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH), thiosemicarbozide (TSC) and osmium tetroxide. J Histochem Cytochem 13:629–639PubMedGoogle Scholar
  25. Spicer SS (1965) Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13:221–234Google Scholar
  26. Spicer SS, Hardin JH, Setser ME (1978) Ultrastructural visualization of sulphated complex carbohydrates in blood and epithelial cells with the high iron diamine procedure. Histochem J 10:435–452PubMedCrossRefGoogle Scholar
  27. Thiéry JP (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J Microsc 6:987–1018Google Scholar
  28. Wetzel BK, Spicer SS, Horn RG (1967) Fine structural localization of acid and alkaline phosphatase in cells of rabbit blood and bone marrow. J Histochem Cytochem 15:311–334PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • H. Sakakibara
    • 1
  • M. Eguchi
    • 1
  1. 1.The Second Department of PediatricsDokkyo University School of MedicineMibu-machi, TochigiJapan

Personalised recommendations