Abstract
Various antisera raised either to antigens ofCandida albicans or to sub-lethal infections of blastospores (convalescent sera) were tested for their efficacy in diagnosing systemic disease in artifically infected animals. Globulin from convalescent serum, when conjugated with alkaline phosphatase and used in enzyme-linked immunosorbent assays (ELISA), was the only antiserum type which detected circulatingCandida-related antigen in the serum of infected animals. Conjugates made from anti-mannan, anti-blastospore or antimycelial globulin did not detect antigen. Mannan did not appear to be related to an antigen produced in sera of experimentally infected mice. The significance of these results in the diagnosis of systemic candidosis is discussed.
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Warren, R.C., Richardson, M.D. & White, L.O. Enzyme-linked immunosorbent assay of antigens from Candida albicans circulating in infected mice and rabbits: The role of mannan. Mycopathologia 66, 179–182 (1979). https://doi.org/10.1007/BF00683968
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DOI: https://doi.org/10.1007/BF00683968