Abstract
More than 300 reference strains representing 60 species and varieties ofFusarium cultures named according to different taxonomic systems are currently maintained at the American Type Culture Collection (ATCC). They have been preserved by freeze-drying and by freezing and subsequent storage in liquid nitrogen (−196°C) to insure their viability without contamination, variation, mutation, or deterioration. The materials and procedures used at the ATCC for the acquisition, accessioning, cataloguing, preservation and distribution are described. Longevity storage data for the strains available for distribution are presented and discussed.
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References
Booth, C. 1971. The genus Fusarium C.M.I. Kew, England. 237 p.
Brown, W., and A.S. Horne 1926. Studies in the genus Fusarium III. An analysis of factors which determine certain microscopic features of Fusarium strains. Ann. Bot.40: 203–221.
Cormack, M.W. 1951. Variation in the cultural characteristics and pathogenicity ofFusarium avanaccum and Fusarium arthrosporioides. Canad. J. Bot.29: 32–45.
Dietz, A. 1975. Nitrogen preservation of stock cultures of unicellular and filamentous microorganisms, pp. 22–36.In: ‘Round table conference on the cryogenic preservation of cell cultures’ (A.P. Rinfred, and B. LaSalle, eds.). Nat'l. Acad. Sci., Washington, D.C.
Fennell, D.I. 1960. Conservation of fungous cultures. Bot. Rev.26: 79–141.
Gordon, W.L. 1952. The occurrence of Fusarium species in Canada. II. Prevalence and taxonomy of Fusarium species in cereal seed. Canad. J. Bot.30: 209–251.
Hossack, D.J.N. 1972. Liquid nitrogen frozen inocula — a year's experience. Proc. Soc. Analyt. Chem.9: 36–38.
Hwang, S.W. 1960. Effects of ultra-low temperature on the viability of selected fungus strains. Mycologia 52: 527–529.
Hwang, S.W., W.F. Kwolet, and W.C. Haynes. 1976. Investigation of ultralow temperature for fungal cultures III. Viability and growth rate of mycełial cultures following cryogenic storage. Mycologia 60: 377–387.
Joffe, A.Z. 1974. A modern system of Fusarium taxonomy. Mycopath. Mycol. Appl. 53: 201–228.
Joffe, A.Z. 1977. The genus Fusarium pp. 59–82.In: ‘Mycotoxic Fungi, Mycotoxins, Mycotoxicoses: An Encyclopedic Handbook’ (T.D. Wyllie & L.G. Morehouse, eds.). Marcel Dekker, New York.
Jong, S.C. 1978a. Conservation of the cultures, pp. 119–135.In: ‘The biology and cultivation of edible mushrooms’ (S.T. Chang & W.A. Hayes, eds.). Academic Press, London and New York.
Jong, S.C. 1978b. Collection of fungi. pp. 198–394.In: ‘Catalogue of strains I’, 13th edition. American Type Culture Collection, Rockville, Maryland.
Miller, J.J. 1945. Studies on the Fusarium of muskmelon wilt. I. Pathogenic and cultural studies with particular reference to the caused and nature of variation in the causal organism. Canad. J. Res.23C: 16–43.
Onions, A.H. 1971. Preservation of fungi. pp. 113–151.In: ‘Methods in microbiology’ (C. Booth, ed.), Vol. 4. Academic Press, New York.
Rodricks, J.W., C.W. Hesseltine, and M.A. Mehlman. (ed.). 1977. Mycotoxins in human and animal health. Pathotox Publishers, Inc., Illinois. 807 p.
Snyder, W.C., and H.N. Hansen. 1940. The species concept in Fusarium Amer. J. Bot. 27: 64–67.
Snyder, W.C., and H.N. Hansen. 1941. The species concept in Fusarium with reference to section Martiella. Amer. J. Bot. 28: 738–742.
Snyder, W.C., and H.N. Hansen. 1945. The species concept in Fusarium with reference to Discolor and other sections. Amer. J. Bot.32: 657–666.
Snyder, W.C., and H.N. Hansen. 1954. Variation and speciation in the genus Fusarium New York Acad. Sci. Ann. 60: 16–23.
Snyder, W.C., and T.A. Toussoun. 1965. Current status of taxonomy in Fusarium species and their perfect stages. Phytopathology 55: 833–837.
Swoager, W.C. 1972. Preservation of microorganisms by liquid nitrogen refrigeration. American Laboratory 4: 45–52.
Tousson, T.A., and P.E. Nelson. 1968. A pictorial guide to the identification of Fusarium species according to the taxonomic system of Snyder & Hansen. Pennsylvania State Univ. Press, University Park. 51 p.
Weiss, F.A. 1957. Maintenance and preservation of cultures, pp. 99–119. In ‘Manual of microbiological methods’ (Society of American Bacteriologists), McGraw-Hill Book Co., Inc., New York.
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Jong, S.C., Davis, E.E. Conservation of reference strains of Fusarium in pure culture. Mycopathologia 66, 153–159 (1979). https://doi.org/10.1007/BF00683964
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DOI: https://doi.org/10.1007/BF00683964