Advertisement

Chemistry of Natural Compounds

, Volume 5, Issue 4, pp 173–175 | Cite as

Preliminary analysis of the composition of polysaccharide fractions

  • Yu. S. Ovodov
  • M. N. Adamenko
Article
  • 66 Downloads

Conclusions

A simple and convenient method for analyzing polysaccharide fractions isolated from natural sources has been proposed.

The method is based on ion-exchange chromatography on DEAE-cellulose and gel filtration on Bio-Gels using small columns and automatic monitoring of the separation with the aid of a flow-through ultraviolet densitometer. The method is characterized by rapidity and good reproducibility.

Keywords

Polysaccharide Alginic Acid Inulin Dextran Sulfate Elution Curve 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    F. Smith and R. Montgomery, The Chemistry of Plant Gums and Mucilages and Some Related Polysaccharides, NY, 31–35, 1959.Google Scholar
  2. 2.
    J. E. Scott, Methods Biochem. Anal.,8, 146, 1960.Google Scholar
  3. 3.
    H. Neukom, H. Deuel, W. J. Heri, and W. Kündig, Helv. Chim. Acta,43, 64, 1960.CrossRefGoogle Scholar
  4. 4.
    S. A. Barker, M. Stacey, and G. Zweifel, Chem. and Ind., 330, 1957.Google Scholar
  5. 5.
    J. Rosik, V. Zitko, and J. Vasatko, Coll.,27, 1346, 1962.Google Scholar
  6. 6.
    D. A. Applegarth and G. S. Dutton, J. Chromatog.,15, 246, 1964.CrossRefGoogle Scholar
  7. 7.
    R. A. Granath, Abhand. Deut. Akad. Wiss., Berlin, Kl. Med., 94, 1964.Google Scholar
  8. 8.
    K. Krinstad and O. Ellefsen, Das Papier,18, 583, 1964.Google Scholar
  9. 9.
    T. A. Miettinen, Clin. Chim. Acta,8, 695, 1963.CrossRefGoogle Scholar
  10. 10.
    D. M. W. Anderson and S. Rahman, Chem. Comm.,8, 145, 1965.Google Scholar
  11. 11.
    D. M. W. Anderson and J. F. Stoddart, Carbohyd. Res.,2, 104, 1966.CrossRefGoogle Scholar
  12. 12.
    D. M. W. Anderson, E. Hirst, and J. F. Stoddart, J. Chem. Soc., 1959, 1966.Google Scholar
  13. 13.
    D. M. W. Anderson, E. Hirst, and J. F. Stoddart, J. Chem. Soc., 1476, 1967.Google Scholar
  14. 14.
    M. Dubois, K. A. Gilles, J. R. Hamilton, P. A. Rebers, and F. Smith, Anal. Chem.,28, 350, 1956.CrossRefGoogle Scholar
  15. 15.
    R. G. Ovodova, V. E. Vaskovsky, and Yu. S. Ovodov, Carbohyd. Res.,6, 328, 1968.CrossRefGoogle Scholar

Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • Yu. S. Ovodov
    • 1
  • M. N. Adamenko
    • 1
  1. 1.Far East Branch, Siberian Division, AS USSRInstitute of Biologically Active SubstancesUSSR

Personalised recommendations