Histochemistry

, Volume 80, Issue 2, pp 157–163 | Cite as

Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VII/RAg in nervous tissue after fixation

  • A. Mauro
  • A. Bertolotto
  • I. Germano
  • G. Giaccone
  • M. T. Giordana
  • A. Migheli
  • D. Schiffer
Article

Summary

The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

Keywords

Public Health Trypsin Formalin Collagenase Laminin 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • A. Mauro
    • 1
  • A. Bertolotto
    • 1
  • I. Germano
    • 1
  • G. Giaccone
    • 1
  • M. T. Giordana
    • 1
  • A. Migheli
    • 1
  • D. Schiffer
    • 1
  1. 1.II. Neurological ClinicUniversity of TurinTurinItaly

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