Abstract
The full-length cDNA corresponding to the mRNA for the hemagglutinin (H) protein of the Yamagata-1 strain of the subacute sclerosing panencephalitis (SSPE) virus was cloned and the nucleotide sequence was determined. The mRNA corresponding to the H protein was composed of 1952 nucleotides and contained a single large open reading frame, which encoded 620 amino acids with a predicted molecular weight of 69,723. This cDNA clone expressed the H protein in Cos 7 cells, and the transfected cells showed hemadsorption. The nucleotide and amino-acid sequence homology with the Edmonston strain of MV were 98.0% and 96.6%, respectively. The deduced amino acid sequence had a single hydrophobic domain near the N-terminus that was long enough to serve as an anchor in the membrane. Five potential glycosylation sites were found on the H protein at identical positions as in the H protein of MV. Cysteine and proline were located at almost identical positions as those of the H protein of MV. In addition, monoclonal antibody study revealed that three epitopes, including the domains that were involved in the biological activities of the H protein of MV, were conserved on the Yamagata-1 strain. These results suggested that the H protein of the Yamagata-1 strain of defective SSPE virus is structurally and functionally similar to that of the Edmonston strain of MV.
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References
WechslerS.L. and MeissnerH.C., Prog Med Virol28, 65–95, 1982.
TrudgettA., GouldE.A., ArmstrongM. MingioliE.S. and McFarlinD.E., Virology109, 180–182, 1981.
terMeulenV., LofflerS., CarterM.J., and StephensonJ.R., J Gen Virol57, 357–364, 1981.
SheshberadaranH., ChenS.-N. and NorrbyE., Virology128, 341–353, 1983.
SatoT.A., FukudaA. and SugiuraA., J Gen Virol66, 1397–1409, 1985.
BaczkoK., LiebertU.G., BilleterM., CattaneoR., BudkaH. and terMeulenV., J Virol59, 472–478, 1986.
LiebertU.G., BaczkoK., BudkaH. and terMeulenV., J Gen Virol67, 2435–2444, 1986.
AlkhatibG. and BriedisD.J., Virology150, 479–490, 1986.
GeraldC., BucklandR., BarkerR., FreemanG. and WildT.F., J Gen Virol67, 2695–2703, 1986.
Horta-BarbosaL., HamiltonR., WittigB., FuccilloD. and SeverJ.L., Science173, 840–841, 1971.
terMeulenV. and HallW.W., J Gen Virol41, 1–25, 1978.
HommaM., TashiroM., KonnoH., OharaY., HinoM. and TakaseS., Microbiol Immunol26, 1195–1202, 1982.
KomaseK., KasaokaT., YoshikawaY., SatoT.A. and YamanouchiK., Virus Genes,4(2): 137–149, 1990.
EndersJ.F. and PeeblesT.C., Proc Soc Exp Biol Med86, 277–286, 1954.
OkayamaH. and BergP., Mol Cell Biol3, 280–289, 1983.
BilleterM.A., BaczkoK., SchmidA. and terMeulenV., Virology132, 147–159, 1984.
HeinkoffS., Gene28, 351–359, 1984.
SangerF., NicklenS. and CoulsonA.R., Proc Natl Acad Sci USA74, 5463–5467, 1977.
BlumbergB., GiorgiC., RouxL., RajuR., DowlingP., CholletA. and KolakofskyD., Cell41, 269–278, 1985.
GiorgiC., BlumbergB.M. and KolakofskyD., Cell35, 829–836, 1983.
GuptaK.C. and KingsburyD.W., Nucleic Acids Res12, 3829–3841, 1984.
ChouP.Y. and FasmanG.D. in MeisterA. (ed.)Advances in Enzymology. Wiley, New York, 1978, pp. 45–148.
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Komase, K., Haga, T., Yoshikawa, Y. et al. Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. III. Nucleotide sequence of the hemagglutinin gene. Virus Genes 4, 163–172 (1990). https://doi.org/10.1007/BF00678407
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DOI: https://doi.org/10.1007/BF00678407