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Molecular and Cellular Biochemistry

, Volume 55, Issue 2, pp 183–190 | Cite as

Grafting of triggering site onto lymphocytes; distribution of grafted dinitrophenyl groups on cell surface glycoproteins and glycolipids

  • Sarah Spiegel
  • Meir Wilchek
Article

Summary

Quantitative investigation of membrane-bound sialoglycoconjugates on lymphocyte surface was performed by chemical modification of the sialic acid residues with radioactive N4-dinitrophenyl-l-2,4-diaminobutyric acid hydrazide (DNP-DABH). This labeled both glycoproteins and glycolipids with concomitant preservation of the mitogenic activity by multivalent hapten binding protein (anti-DNP antibody). Under conditions where maximum stimulation of thymocytes occurred radioactive DNP-DABH labeled 1.1 × 107 glycolipids molecules/cell but, only 3 × 106 glycoproteins molecules/cell. When B lymphocytes, which do not undergo DNP-mediated stimulation were used, glycolipids labeling could not be detected. Major differences between stimulation committed and non-committed DNP-modified lymphocytes was the amount of ligand attached to the cell surface sialoglycolipids (gangliosides).

Keywords

Cell Surface Sialic Acid Chemical Modification Hydrazide Surface Glycoprotein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DNP

dinitrophenyl

DNP-DABH

N4-dinitrophenyl-l-2,4-diaminobutyric acid hydrazide

PHA

phytohemagglutinin from the red kidney beanPhaseolus vulgaris

Con A

concanavalin A

PBS

phosphate buffered saline, pH 7.2

FDNB

1-fluoro-2,3-dinitrobenzene

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Copyright information

© Martinus Nijhoff Publishers 1983

Authors and Affiliations

  • Sarah Spiegel
    • 1
  • Meir Wilchek
    • 1
  1. 1.Department of BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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