Archiv für Gynäkologie

, Volume 209, Issue 3, pp 276–292 | Cite as

Versuche mit Rinder- und Schweinekeimzellen zur Reifung, Befruchtung und Embryonenzüchtung in vitro

  • K. Bregulla
  • H. Bernecker
  • K. Adler
  • R. Hahn
Article

Zusammenfassung

Aus Rinder- und Schweineovarien (Schlachttiere) wurden Oocyten durch eine Follikelpunktion entweder sofort nach der Schlachtung oder bis zu einem Zeitraum von 4 Std danach entnommen. Falls die Punktion nicht unmittelbar nach dem Herauspräparieren der Organe möglich war, wurden die Ovarien auf Eis bis zur weiteren Verarbeitung gelagert.

Die extracorporale Reifung, Befruchtung und Embryonenzüchtung wurde versucht.

Der Unterschied zwischen den jeweils gewonnenen und ausgewerteten Eiern in den entsprechenden Versuchen war durch die Präparierarbeiten bedingt.

Von 1003 follikulären Rindereiern kamen 539 zur Auswertung. In 13 Oocyten war 1, in 2 Oocyten waren 2 und in 1 Oocyten waren 3 Polkörperchen sichtbar. Zwei Eizellen hatten geteilte Kerne („Zweizellstadium“).

Bei den follikulären Schweineoocyten konnten von 851 Eiern 565 ausgewertet werden. In einer Oocyte wurde ein und in 3 Oocyten wurden zwei Polkörperchen gesehen. Vier follikuläre Schweineeier waren geteilt („Zweizellstadium“), eine Zellteilung erfolgte ohne Spermazugabe.

Die 122 tubaren Schweineoocyten wurden durch eine Tubenspülung kurz nach der Ovulation gewonnen. Davon waren unmittelbar nach dem Ausspülen 28 geteilt und 94 ungeteilt. Die Teilung dieser Eizellen war sicher degenerativ (Fragmentation). Die ungeteilten tubaren Oocyten wurden mit und ohne Sperma kultiviert.

An einer gelungenen extracorporalen Reifung sowohl der follikulären Rinder- und Schweineoocyten als auch der tubaren Schweineoocyten besteht kein Zweifel. Die Befruchtung und die Züchtung der Embryonen wird nicht bejaht, da der letzte Beweis, eine Transplantation der fraglichen Embryonen auf ein artgleiches nidationsreifes Endometrium mit dem Wachsen einer normalen Fruchtanlage, nicht geführt wurde.

Mikrophotographien dokumentieren die Versuchsergebnisse.

Experiments with germ-cells of cows and pigs for in vitro maturation, fertilization and cultivation of embryos

Summary

From the ovaries of cows and pigs (animals for slaughter) oocytes were picked up by puncturing of a follicle either immediately after the slaughter has taken place or until 4 hours thereafter. In case puncture was not possible immediately after the organs had been removed the ovaries were placed on ice until they were processed further. The extracorporal maturation, fertilization and cultivation of embryos were attempted. The difference between the obtained eggs and those which could be evaluated in corresponding experiments was caused by the preparatory manipulation.

Of 1003 follicular cow ova 539 were evaluated.

In 13 oocytes there was 1, in 2 oocytes were 2 and in 1 oocyte were 3 polar bodies visible.

Two of the ova showed a divided nucleus (“Two-cell state”). From the follicular pig oocytes 565 out of 851 could be evaluated. In one of the oocytes one single and in 3 of the oocytes 2 polar bodies were observed. Four follicular pig eggs were divided (“two-cell state”) one of the cell division had occurred without application of sperm. 122 tubal pig oocytes were obtained by rinsing the Fallopian tubes shortly after ovulation. Of those 28 were found to be divided and 94 undivided immediately after the rinsing. The division of these cells occurred degeneratively for certain (fragmentation). The undivided tubal oocytes were cultivated with and without sperm cells. There is no doubt about a successful extracorporal maturation as well of the follicular cow- and pig oocytes as of the tubal pig oocytes. Fertilization and cultivation of embryos are not affirmed, as the ultimate proof, a transplantation of the embryos in question to an endometrium of identical species and ripe for nidation followed by growth of a normal embryo could not be brought.

Microphotographs are documenting the experimental results.

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Copyright information

© J. F. Bergmann 1970

Authors and Affiliations

  • K. Bregulla
    • 1
    • 2
  • H. Bernecker
    • 1
    • 2
  • K. Adler
    • 1
    • 2
  • R. Hahn
    • 1
    • 2
  1. 1.Universitäts-FrauenklinikErlangen-Nürnberg
  2. 2.Besamungszentrale Neustadt/AischGermany

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