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Histochemische Untersuchungen über Hydroxysteroid Dehydrogenase-Aktivität in Nebennieren und Eierstöcken von Ratten und Kaninchen

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Zusammenfassung

In Nebennieren und Eierstöcken geschlechtsreifer und infantiler Kaninchen und Ratten wurde die histochemische Darstellung der Hydroxysteroid-Dehydrogenase-Aktivität vorgenommen und das histochemische Verhalten der Enzymaktivität nach Hypophysektomie, Kastration, Gonadotropin- und Oestrogengaben untersucht. In allen hormonproduzierenden Zellen endokriner Organe ist eine 3β-ol Steroid-Dehydrogenase-Aktivität nachweisbar. Nach Gonadotropingaben steigt die Enzymaktivität in den Eierstöcken an, nach Hypophysektomie sinkt die Aktivität in NN und Ovarien ab. Es wird versucht, die enzymhistochemischen Befunde mit den Ergebnissen der Untersuchungen über die Biosynthese der Steroidhormone in Beziehung zu setzen.

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Literatur

  1. Baggett, B., L. L. Engel, L. Balderas, G. Lanman, K. Savard andR. J. Dorfman: Conversion of C4 Testosterone to C14-estrogenic steroids by endocrine tissues. Endocrinology64, 600 (1959).

    Google Scholar 

  2. Brady, R. O.: Biosynthesis of radioactive testosteron in vitro. J. biol. Chem193, 145 (1951).

    Google Scholar 

  3. Burstein, S.: Zit. bei S.Lieberman u. R. V.Wiele, Dehydroisoandrosterone, its origin and importance as a precursor of urinary 17-ketosteroids. Proc. IV. Internat. Congr. of Biochemistry 1958, S. 153. London: Pergamon Press.

    Google Scholar 

  4. Culinar, A.: Zit.10. J. Obstet. Gynaec. Brit. Emp.52, 545 (1954).

    Google Scholar 

  5. Dorfman, R. J.: Metabolism of androgenes. Proc. IV. Internat. Congr. of Biochemistry 1958, S. 175 Sympos. IV. London: Pergamon Press 1958.

    Google Scholar 

  6. —, andF. Ungar: Metabolism of steroid hormones. Minneapolis: Burgess publishing & Co. 1953.

    Google Scholar 

  7. Faber, E., W. H. Sternberg andC. E. Dunlap: Tetrazolium strains for diphosphopyridine nucleotide (DPN) diaphorase and triphosphopyridin nucleotide diaphorase in animal tissues. Proc. Soc. exp. Biol. (N.Y.)86, 534 (1954).

    Google Scholar 

  8. — — —: Histochemical localisation of specific oxidative enzymes. I. Tetrazolium strains for diphosphopyridin nucleotide diaphorases and triphosphoridine nucleotide diaphorases. J. Histochem. Cytochem.4, 254 (1956).

    Google Scholar 

  9. Fuhrmann, K.: Über den histochemischen Nachweis der 3β-ol-Steroiddehydrogenasen in Geweben endokriner Organe. Zbl. Gynäk.83, 565 (1961).

    Google Scholar 

  10. Junkmann, K.: Die Androgene des Ovars. In: Moderne Entwicklungen auf dem Gestagengebiet, S. 112. Berlin-Göttingen-Heidelberg: Springer 1960.

    Google Scholar 

  11. Kellog, D. A., andG. G. Glenner: Histochemical localisation of human-term placental 17-β-oestradiol dehydrogenases: implications for the transhydrogenase reaction. Nature (Lond.)187, 763 (1960).

    Google Scholar 

  12. Kochakian, Ch. D.: Metabolism of androgenes by tissue enzymes. In: Biochemistry of steroids in Proc. of IV. Internat. Congr. of Biochemistry. London: Pergamon Press 1958, S. 196.

    Google Scholar 

  13. Langer, L. J., andL. L. Engel: Human placental estradiol-17β-dehydrogenase I u. II. J. biol. Chem.233, 583 (1958);234, 2609 (1959).

    Google Scholar 

  14. Levy, H., H. Wendler Deane andB. L. Rubin: Visualization of steroid-3β-ol dehydrogenase activity in tissues of intact and hypophysectomized rats. Endocrinology65, 932 (1959).

    Google Scholar 

  15. Lieberman, S., andR. C. Wiele: Dehydroisoandrosterone, its origin and importance as a precursor of urinary 17-ketosteroids. Proc. of the IV. Internat. Congr. of Biochemistry. London: Pergamon Press 1958, S. 153.

    Google Scholar 

  16. Marcus, P. J., andP. Talalay: Induction and purification of α- andβ-hydroxysteroid dehydrogenases. J. biol. Chem.218, 661 (1956).

    Google Scholar 

  17. Migeon, C. J., andJ. E. Plager: Identification and isolation of dehydroepiandrosteron from peripheral human plasma. J. biol. Chem.209, 767 (1954).

    Google Scholar 

  18. Nachlas, M. M., K. C. Tsou, E. De Souza, C.-S. Cheng andA. M. Seligman: Cytochemical demonstration of succinic dehydrogenase by use of new p-nitrophenyl substituted ditetrazol. J. Histochem. Cytochem.5, 420 (1957).

    Google Scholar 

  19. —,D. G. Walker andA. M. Seligman: Histochemical method for demonstration of diphosphoridin nucleotid diaphorase. J. biophys. biochem. Cytol.4, 29 (1958).

    Google Scholar 

  20. — — —: Histochemical localisation of triphosphoridinnucleotide diaphorase. J. biophys. biochem. Cytol.4, 467 (1958).

    Google Scholar 

  21. Novikoff, A. B.: Histochemical and cytochemical staining methods in analytical cytology, R. C. Mellors Ed. New York: Mc Graw-Hill & Co. 1955.

    Google Scholar 

  22. Oertel, G. W., andK. Eik-Nes: Determination of dehydroepiandrosteron in human blood plasma. J. biol. Chem.232, 543 (1958).

    Google Scholar 

  23. — —: Plasma levels of dehydroepiandrosterone in the dog. Endocrinology65, 766 (1959).

    Google Scholar 

  24. Orsinski, P. A.: Steroid 11β-ol dehydrogenase in human placenta. Nature (Lond.)187, 777 (1960).

    Google Scholar 

  25. Paschkis, K. E., andA. E. Rakoff: Recent Progr. Hormone Res.5, 115 (1950).

    Google Scholar 

  26. Pearlman, W. H., E. Cerceo andM. Thomas: The conversion ofΔ 5-pregnen-3β-ol-20-one to progesteron by homogenates of human placental tissue. J. biol. Chem.208, 231 (1954).

    Google Scholar 

  27. Pearson, B., andF. Grose: Histochemical demonstration of 17-β-hydroxysteroid dehydrogenase by use of tetrazolium salt. Proc. Soc. exp. Biol. (N.Y.)100, 636 (1959).

    Google Scholar 

  28. Price, D.: Comparison of reactions of male and female rat prostate transplants. Anat. Rec.82, 93 (1942).

    Google Scholar 

  29. Rauschkolb, E. W., G. L. Farrel andS. Koletsky: Androsteron secretions after hypophysectomie. Amer. J. Physiol.184, 55 (1956).

    Google Scholar 

  30. Rennels, E. C.: Influence of hormones on histochemistry of ovarian interstitial tissue of immature rat. Amer. J. Anat.88, 61 (1951).

    Google Scholar 

  31. Rubin, B. L., andR. J. Dorfman: Quantitative assay of the 3β-ol-dehydrogenase system in whole adrenal homogenate. Endocrinology61, 601 (1957).

    Google Scholar 

  32. Ryan, K. J.: Conversion ofΔ 5-androstene-3β, 17β-triol to estriol by human placenta. Endocrinology63, 392 (1958).

    Google Scholar 

  33. —: Biological aromatization of steroide. J. biol. Chem.234, 268 (1959).

    Google Scholar 

  34. Samuels, L. T., M. L. Helmreich, M. B. Lasatera andH. Reich: An enzyme in endocrine tissues which oxidivesΔ 5-3-hydroxy steroids to α,β unsaturated ketones. Science113, 490 (1951).

    Google Scholar 

  35. — —: The influence of chorionic gonadotropin on the 3β-ol dehydrogenase activity of testes and adrenals. Endocrinology58, 435 (1956).

    Google Scholar 

  36. Seligman, A. M., andA. M. Rutenberg: The histochemical demonstration of succinic dehydrogenase. Science113, 317 (1951).

    Google Scholar 

  37. Shelton, E., andW. C. Schneider: On the usefulness of tetrazolium salts as histochemical indicators of dehydrogenase activity. Anat. Rec.112, 61 (1952).

    Google Scholar 

  38. Smith, F. E.: Tetrazolium salt. Science113, 751 (1951).

    Google Scholar 

  39. Schultz-Brauns, O.: Eine neue Methode des Gefrierschneidens für histologische Schnelluntersuchungen. Klin. Wschr.10, 113 (1931).

    Google Scholar 

  40. Stachenko, J., andCl. J. P. Giroud: Functional zonation of the adrenal cortex: pathways of corticosteroid biogenesis. Endocrinology64, 730 (1959).

    Google Scholar 

  41. Stone, D., andO. Hechter: Studies on ACTH action in perfused bovines adrenals site of action of ACTH corticosteroidogenesis. Arch. Biochem.51, 457 (1954).

    Google Scholar 

  42. Taber, E.: Androgen secretion in the fowl. Endocrinology48, 6 (1951).

    Google Scholar 

  43. Talalay, P., andR. H. Levy: Steric and molecular specifity of steroid dehydrogenases. Ciba Foundation Study Group 2, 53 (1959). London: J. A. Churchill 1959.

    Google Scholar 

  44. —, andP. J. Marcus: Specifity, kinetics and inhibition of α- andβ-hydroxy steroid dehydrogenes. J. biol. Chem.218, 675 (1956).

    Google Scholar 

  45. —, andH.-G. Williams-Ashman: Participation of steroid hormones in the enzymatic transfer of hydrogen. Rec. Progr. in Hormone Research 1960, p. 1. New York and London: Academic Press 1960.

    Google Scholar 

  46. Tomkins, G. M.: A mammalian 3-α-hydroxysteroid dehydrogenase. J. biol. Chem.218, 437 (1956).

    Google Scholar 

  47. Tsou, K. C., C.-S. Cheng, M. M. Nachlas andA. M. Seligman: Syntheses of some p-nitrophenyl substituted tetrazolium salts as electron acceptors, for demonstration of dehydrogenases. J. Amer. chem. Soc.78, 6139 (1956).

    Google Scholar 

  48. Wachstein, M., andE. Meisel: Thin sections from unfixed tissues for histochemical staining. Stain Technol.28, 135 (1953).

    Google Scholar 

  49. Wattenberg, L. W.: Microscopic histochemical demonstration of steroid-3β-ol dehydrogenase in tissue sections. J. Histochem. Cytochem.6, 225 (1958).

    Google Scholar 

  50. Zander, J.: Die gestagen wirksamen Hormone im Organismus. Geburtsh. u. Frauenheilk.17, 876 (1957).

    Google Scholar 

  51. Zweifach, B. W., M. M. Black andE. Shorr: Histochemical alterations revealed by tetrazolium chloride in hypertensive kidneys in relation to renal VEM mechanism. Proc. Soc. exp. Biol. (N.Y.)74, 848 (1950).

    Google Scholar 

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  1. Universitäts-Frauenklinik Erlangen, Erlangen

    K. Fuhrmann

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Auszugsweise vorgetragen auf dem III. Weltkongreß für Geburtshilfe und Gynäkologie, Wien 1961.

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Fuhrmann, K. Histochemische Untersuchungen über Hydroxysteroid Dehydrogenase-Aktivität in Nebennieren und Eierstöcken von Ratten und Kaninchen. Arch. Gynak. 197, 583–600 (1963). https://doi.org/10.1007/BF00668401

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  • DOI: https://doi.org/10.1007/BF00668401

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