Breast Cancer Research and Treatment

, Volume 35, Issue 2, pp 195–199 | Cite as

Prognostic significance of laminin production in relation with its receptor expression in human breast carcinomas

  • Rita Pellegrini
  • Stefania Martignone
  • Elda Tagliabue
  • Dorina Belotti
  • Rosaria Bufalino
  • Natale Cascinelli
  • Sylvie Ménard
  • Maria Ines Colnaghi


Laminin is a basement membrane glycoprotein whose expression has been widely related to cancer progression. Laminin production by primary breast carcinomas was investigated using immunohistochemistry on archival specimens from a retrospective series with long term follow-up. Laminin production was found to be independent of the clinical and pathological variables analyzed, whereas a statistically significant direct association with the expression of the laminin receptor and a negative association with the differentiation-related antigen Ca-MBr8 were observed. Survival analysis indicated that laminin positivity by itself has no prognostic significance. However, when analyzed together with the laminin receptor expression, laminin was associated with a good prognosis in receptor-negative tumors and with the worst prognosis in receptor-positive tumors.

Key words

breast cancer laminin laminin receptor metastasis prognosis 


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  1. 1.
    Timpl R, Engel J, Martin GR: Laminin: a multifunctional protein of basement membranes. Trends Biochem 8: 207–209, 1983Google Scholar
  2. 2.
    Kleinman HK, Cannon FB, Laurie JR, Hassell JR, Aumailley M, Terranova VP, Martin GR, Dubois-Dalco M: Biological activities of laminin. J Cell Biochem 27: 317–325, 1985Google Scholar
  3. 3.
    Castronovo V: Laminin receptors and laminin-binding proteins during tumor invasion and metastasis. Invasion Metastasis 13: 1–30, 1993Google Scholar
  4. 4.
    Ruoslahti E: Integrins. J Clin Invest 87: 1–5, 1991Google Scholar
  5. 5.
    Rao CN, Barsky SH, Terranova VP, Liotta LA: Isolation of a tumor cell laminin receptor. Biochem Biophys Res Commun 111: 804–808, 1983Google Scholar
  6. 6.
    Varani J, Lovett EJ, McCoy JP, Shibata S, Maddose DE, Goldstein IJ, Wicha M: Differential expression of a lamininlike substance by high and low metastatic tumor cells. Am J Pathol 111: 27–34, 1983Google Scholar
  7. 7.
    Turpeenniemi HT, Thorgeirsson UP, Rao CN, Liotta LA: Laminin increases the release of type IV collagenase from malignant cells. J Biol Chem 261: 1883–1889, 1986Google Scholar
  8. 8.
    Castronovo B, Parent P, Foidart JM, Heynen E, Goffinet G, Lambotte R, Mahieu P: Perfusion of human umbilical veins. A new approach to study the interactions of circulating malignant cells with vascular wall and their modulation. Invasion Metastasis 8: 332–350, 1988Google Scholar
  9. 9.
    Vollmers HP, Imhof BA, Braun S, Waller CA, Schirrmacher V, Birchmeier W: Monoclonal antibodies which prevent experimental lung metastasis in a murine model. FEBS Lett 172: 17–20, 1984Google Scholar
  10. 10.
    Albrechtsen R, Nielsen M, Wewer U, Engvall E, Ruoslahti E: Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin. Cancer Res 41: 5076–5081, 1981Google Scholar
  11. 11.
    D'Ardenne AJ, Kirkpatrick P, Wells CA, Davies JD: Laminin and fibronectin in adenoid cystic carcinoma. J Clin Pathol 39: 138–144, 1986Google Scholar
  12. 12.
    D'Ardenne AJ, Richman PI, Horton MA, MCaulay AE, Jordan S: Co-ordinate expression of the alpha-6 integrin laminin receptor subunit and laminin in breast cancer. J Pathol 165: 213–220, 1991Google Scholar
  13. 13.
    Terranova VP, Rao CN, Kalebic T, Margulies IM, Liotta LA: Laminin receptor on human breast carcinoma cells. Proc Natl Acad Sci USA 80: 444–448, 1983Google Scholar
  14. 14.
    Castronovo V, Taraboletti G, Liotta LA, Sobel ME: Modulation of laminin receptor expression by estrogen and progestins in human breast cancer cell lines. J Natl Cancer Inst 81: 781–788, 1989Google Scholar
  15. 15.
    Marques AL, Franco ELF, Torloni H, Brentani MM, Da Silva-Neto JB, Brentani RR: Independent prognostic value of laminin expression in breast cancer survival. Cancer Res 50: 1479–1483, 1990Google Scholar
  16. 16.
    Daidone MG, Silvestrini R, D'Errico A, Di Fronzo G, Benini E, Mancini AM, Garbisa S, Liotta LA, Grigioni WF: Laminin receptors, collagenase IV and prognosis in node-negative breast cancers. Int J Cancer 48: 529–532, 1991Google Scholar
  17. 17.
    Martignone S, Ménard S, Bufalino R, Cascinelli N, Pellegrini R, Tagliabue E, Andreola S, Rilke F, Colnaghi MI: Prognostic significance of the 67-kilodalton laminin receptor expression in human breast carcinomas. J Natl Cancer Inst 85: 398–402, 1993Google Scholar
  18. 18.
    Mariani-Costantini R, Barbanti P, Colnaghi MI, Ménard S, Clemente C, Rilke F: Reactivity of a monoclonal antibody with tissues and tumors from human breast: Immunohistochemical localization of a new antigen and clinico-pathologic correlations. Am J Pathol 115: 47–56, 1984Google Scholar
  19. 19.
    Mastroianni A, Invernizzi L, Tagliabue E, Fassina G, Albini A, Ménard S, Colnaghi MI: Alteration of laminin production in small-cell lung carcinoma: possible correlation with the absence of the basement membrane. Tumor Biol 14: 279–287, 1993Google Scholar
  20. 20.
    Facheris P, Perrone F, Ménard S, Andreola S, Bazzini P, Bufalino R, Canevari S, Cascinelli N, Colzani E, Di Fronzo G, Colnaghi MI: Study of the biological and prognostic significance of the antigen CaMBr8 on breast carcinoma. Br J Cancer 65: 466–470, 1992Google Scholar
  21. 21.
    Martignone S, Pellegrini R, Villa E, Tandon NN, Mastroianni A, Tagliabue E, Ménard S, Colnaghi MI: Characterization of two monoclonal antibodies directed against the 67 KDa high affinity laminin receptor and application for the study of breast carcinoma progression. Clin Exp Metast 10: 379–386, 1992Google Scholar
  22. 22.
    Barsky SH, Rao CN, Williams JE, Liotta LA: Laminin molecular domains which alter metastasis in a murine model. J Clin Invest 74: 843–848, 1984Google Scholar
  23. 23.
    Malinoff HL, McCoy JP, Varani J, Wicha MS: Metastatic potential of murine fibrosarcoma cells is influenced by cell surface laminin. Int J Cancer 33: 651–655, 1984Google Scholar
  24. 24.
    Taraboletti G, Belotti D, Giavazzi R, Sobel ME, Castronovo V: Enhancement of metastatic potential of murine and human melanoma cells by laminin receptor peptide G: Attachment of cancer cells to subendothelial matrix as a pathway for hematogenous metastasis. J Natl Cancer Inst 85: 235–240, 1993Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Rita Pellegrini
    • 2
  • Stefania Martignone
    • 2
  • Elda Tagliabue
    • 2
  • Dorina Belotti
    • 1
    • 2
  • Rosaria Bufalino
    • 2
  • Natale Cascinelli
    • 2
  • Sylvie Ménard
    • 2
  • Maria Ines Colnaghi
    • 2
  1. 1.Istituto Mario NegriBergamoItaly
  2. 2.Division of Experimental Oncology EIstituto Nazionale TumoriMilanItaly

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