Summary
An in vitro ovulation could be induced and observed in four human ovaries with Graafian follicle. Perfusion was carried out by means of a semi-synthetic medium which was free from haemoglobin in a closed recirculating system. During the stimulation by means of HCG/HMG (after the II. IRP a total of 22.9 I.U. LH/ml and 3 I.U. FSH/ml) an activation of respiration and glycolysis by a factor of 2–3up to the rupture of the follicle was observed. In this process 93% of the energy were released by the oxydative reduction of glucose and only about 7% by anaerobic glycolysis. 60–90 minutes before the rupture of the follicle an undulatory rise in the arterial hydrostatic pressure of up to 40 mmHg over the initial values was observed, although the outward conditions of perfusion were not altered, after the rupture a pressure drop to standard values. Apart from minor oscillations there was no increase in the intrafollicular pressure in spite of an increase in volume of the follicle, but only a decrease in pressure after the rupture of the follicle.
Zusammenfassung
An vier menschlichen Ovarien konntein vitro unter der Stimulation mit HCG/HMG eine Ovulation induziert und beobachtet werden. Die Ovarien wurden in einem rezirkulierenden System mit einem hämoglobinfreien, halbsynthetischen Medium perfundiert. Unter der Stimulation nahmen Atmung und Glykolyse bis zur Ruptur des Follikels um einen Faktor 2–3 zu. Dabei wurden aber −93% der freiwerdenden Energie aus dem aeroben Glucoseabbau gewonnen und nur −7% durch anaerobe Glykolyse. In der direkt präovulatorischen Phase konnte trotz Volumenzunahme des Follikels kein Anstieg des intrafollikulären hydrostatischen Druckes beobachtet werden. Der arterielle hydrostatische Druck dagegen zeigte 60–90 min zuvor einen wellenförmigen Anstieg bis zu 40 mmHg über den Normallevel, nach der Follikelruptur Abfall auf Ausgangswerte. Die Ergebnisse werden im Hinblick auf den Ovulationsmechanismus diskutiert.
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Die Untersuchungen wurden mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt.
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Stähler, E., Spätling, L., Bethge, H.D. et al. Induction of ovulation in human ovaries perfusedin vitro . Arch. Gynak. 217, 1–15 (1974). https://doi.org/10.1007/BF00666637
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DOI: https://doi.org/10.1007/BF00666637