Archives of oto-rhino-laryngology

, Volume 230, Issue 1, pp 81–92 | Cite as

Cytoskeletal and muscle-like elements in cochlear hair cells

  • Hans P. Zenner
Article

Summary

Monospecific antibodies to actin and to tubulin were used as immunofluorescent probes to evaluate the distribution of microtubules and actin filaments in the organ of Corti in mouse and guinea pig. The results indicate that in cochlear receptor cells actin and actin filaments as well as tubulin and microtubules are integral cytoskeletal elements. The presence of actin suggests a possible contractile mechanism within the sensory cilia whereas tubulin is thought to play an important role in the stability of sensory cells. Both proteins are discussed to form structural elements required for the mechano-chemical coupling in hearing.

Key words

Hearing cells Stability Actin Tubulin Cytoskeleton Mechano-chemical coupling 

Abbreviations

ATP

adenosin-tri-phosphate

SDS

sodium-dodecyl-sulphate

PBS

phosphate-buffered saline

Aktin und Tubulin — strukturelle und kontraktile Moleküle in den Sinneszellen der Cochlea

Zusammenfassung

Monospezifische Antikörper gegen Aktin und Tubulin wurden in Kaninchen induziert und zum Nachweis von Mikrotubuli und Mikrofilamenten im Cortischen Organ von Mäusen und Meerschweinchen angewandt. Die Ergebnisse zeigen, daß Tubulin und Mikrotubuli Strukturelemente der Hörzellen sind, die deren Zytoskelett bilden. Während Mikrotubuli vorwiegend für die mechanische Stabilität der Haarzellen verantwortlich gemacht werden, läßt der Nachweis von Aktin die Möglichkeit eines kontraktilen Mechanismus in den Stereozilien zu. Für beide Proteine wird eine wesentliche Rolle bei der mechano-chemischen Kopplung des Hörorgans diskutiert.

Schlüsselwörter

Hörzellen Stabilität Aktin Tubulin Zytoskelett Mechano-chemische Kopplung 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Hans P. Zenner
    • 1
  1. 1.Department of OtolaryngologyUniversity of Würzburg Medical SchoolWürzburgFederal Republic of Germany

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