Theoretical and Applied Genetics

, Volume 69, Issue 3, pp 297–304 | Cite as

Genetic analysis of instability inPetunia hybrida

3. Periclinal chimeras resulting from frequent mutations of unstable alleles
  • M. Doodeman
  • F. Bianchi
Originals

Summary

InPetunia hybrida frequent mutations of unstable alleles give rise to different types of periclinal chimeras. If genes expressed in the epidermis, such as the geneAn1 for flower colour, are concerned, mutations in the dermal layer of the shoot apex will result in changes in the phenotype but not in the offspring. Mutations in the subdermal layer will not lead to an altered phenotype, but to changes in the sporogenous tissues and, thus, to deviating segregations in progenies. Therefore, in crossing experiments with such an unstable mutant, it is always necessary to take the possibility into account that the plant may be a chimera, so as to prevent an incorrect interpretation of the recorded segregational ratios. Mutations of unstable alleles expressed in the mesophyll, such as geneYg3 for leaf colour, also give rise to chimeras. In such instances, however, a change in phenotype always involves a change in segregational ratios as well, since both the mesophyll and the sporogenous tissues are derived from the subdermal layer of the shoot apex.

Key words

Periclinal chimeras Unstable alleles Petunia hybrida 

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References

  1. Bianchi F, Walet-Foederer HG (1974) An investigation into the anatomy of the shoot apex ofPetunia hybrida in connection with the results of transformation experiments. Acta Bot Neerl 23:1–6Google Scholar
  2. Bianchi F, de Boer R, Pompe AJ (1974) An investigation into spontaneous reversions in a dwarf mutant ofPetunia hybrida in connection with the interpretation of the results of transformation experiments. Acta Bot Neerl 23:691–700Google Scholar
  3. Bianchi F, Cornelissen PTJ, Gerats AGM, Hogervorst JMW (1978) Regulation of gene action inPetunia hybrida: unstable alleles of a gene for flower colour. Theor Appl Genet 53:157–167Google Scholar
  4. Cornu A, Bugnon F (1971) Un exemple de ramification résolutive chez les phanérogames: formation de 1-inflorescence scorpioïde chez lePetunia hybrida Hort. Mem Soc Bot Fr (1905–1921) 1971:87–98Google Scholar
  5. Doodeman M, Boersma EA, Koomen W, Bianchi F (1984a) Genetic analysis of instability inPetunia hybrida. I. A highly unstable mutation induced by a transposable element inserted at theAn1 locus for flower colour. Theor Appl Genet 67:345–355Google Scholar
  6. Doodeman M, Gerats AGM, Schram AW, de Vlaming P, Bianchi F (1984b) Genetic analysis of instability inPetunia hybrida. 2. Unstable mutations at different loci as the result of transpositions of the genetic element inserted at theAn1 locus. Theor Appl Genet 67:357–366Google Scholar
  7. Gerats AGM, Cornelissen RTJ, Groot S, Hogervorst JMW, Schram AW, Bianchi F (1982) A gene controlling rate of anthocyanin synthesis and mutation frequency of the geneAn1 inPetunia hybrida. Theor Appl Genet 62:199–203Google Scholar
  8. Krüger M (1932) Vergleichend-entwicklungsgeschichtliche Untersuchungen an den Fruchtknoten und Früchten zweierSolanum-Chimären und ihrer Elternarten. Planta 17:372–436Google Scholar
  9. Satina S, Blakeslee AF (1941) Periclinal chimeras inDatura stramonium in relation to development of leaf and flower. Am J Bot 28:862–871Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Doodeman
    • 1
  • F. Bianchi
    • 1
  1. 1.Department of GeneticsUniversity of AmsterdamAmsterdamThe Netherlands

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