Summary
Seed from homozygous recessivems 1 genetic male-sterile soybean (Glycine max (L.) Merr.) plants was studied for frequencies of polyembryonic seedlings and different levels of polyploidy among abnormal seedlings from six different source populations: Amesms 1 (Ams), North Carolinams 1 (NCms), Tonicams 1 (Tms), Urbanams 1 (Ums), and F4 generation seed obtained from crosses ofms 1 to two chromosome interchange lines (Ams x Clark T/T and Ums x KS-172-11-3). Frequencies of polyembryony observed in Tms, Ums, Ams, NCms, F4 seed from Ams x Clark T/T, and F4 seed from Ums x KS-172-11-3 were 3.6%, 2.4%, 3.1%, 2.5%, 2.2% and 0.1%, respectively. Frequencies of abnormal seedlings from these six sources varied from 1.7% (Ums X KS-172-11-3) to 16.8% (Ams X Clark T/T). Frequencies of polyploids among the abnormal seedlings ranged from 6.8% in Ums x Ks-172-11-3 to 66.7% in Tms. On average, the frequency of polyploid individuals from monoembryonic seedlings was 1.22%. Chromosome number of these seedlings varied from 20 to 200. Variation of the frequencies of polyembryonic seedlings and polyploid progeny among abnormal seedlings suggested that the mechanism(s) controlling the characters of polyembryony and formation of polyploids was associated with thems 1 gene and was affected by other gene(s) or environmental factors.
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References
Albertsen MC (1976) Developmental cytology of microsporogenesis of thems 1 mutant in soybeans,Glycine max (L.) Merr. MSc Thesis, Iowa State University Library, Ames
Albertsen MC, Palmer RG (1979) A comparative light-and electron-microscopic study of microsporogenesis in male-sterile (ms 1) and male-fertile soybeans (Glycine max (L.) Merr). Am J Bot 66:253–265
Beversdorf WD, Bingham ET (1977) Male sterility as a source of haploids and polyploids ofGlycine max. Can J Genet Cytol 19:283–287
Boerma HR, Cooper RL (1978) Increased female fertility associated with thems 1 locus in soybeans. Crop Sci 18: 344–346
Brim CA, Young MF (1971) Inheritance of a male-sterile character in soybeans. Crop Sci 11:564–566
Caviness CE, Walters HJ, Johnson DL (1970) A partially male sterile strain of soybean. Crop Sci 1:107–108
Crane CF, Beversdorf WD, Bingham ET (1982) Chromosome pairing and associations at meiosis in haploid soybean (Glycine max). Can J Genet Cytol 24:293–300
Cutter GL (1975) Effect of a genetic male-sterile character on the organization and function of the female gametophyte in soybeans. PhD Diss, University of Wisconsin, Madison
Cutter GL, Bingham ET (1977) Effect of soybean male-sterile genems 1 in organization and function of the female gametophyte. Crop Sci 17:760–764
Delannay X, Palmer RG (1982) Genetics and cytology of thems 4 male-sterile soybean. J Hered 73:219–223
Graybosch RA, Bernard RL, Cremeens CR, Palmer RG (1984) Genetic and cytological studies on a male-sterile, female-fertile mutant inGlycine max (L.) Merr. (Soybean). J Hered (in press)
Hadley HH, Starnes WJ (1964) Sterility in soybeans caused by asynapsis. Crop Sci 4:421–424
Johns CW, Palmer RG (1982) Floral development of a flower-structure mutant in soybeans,Glycine max (L.) Merr. (Leguminosae). Am J Bot 68:829–842
Kenworthy WJ, Brim CA, Wernsman EA (1973) Polyembryony in soybeans. Crop Sci 13:637–638
Owen FV (1928a) A sterile character in soybeans. Plant Physiol 3:223–226
Owen FV (1928b) Soybean seeds with two embryos. J Hered 19:373–374
Palmer RG (1974) A desynaptic mutant in the soybean. J Hered 65:280–286
Palmer RG, Heer H (1973) A root tip squash technique for soybean chromosomes. Crop Sci 13:389–391
Palmer RG, Heer H (1976) Polyploids among the progeny from male sterile (ms 1 ms 1) soybeans. Soybean Genet Newslett 3:35–36
Palmer RG, Winger CL, Albertsen MC (1978) Four independent mutations at thems 1 locus in soybeans. Crop Sci 18:727–729
Palmer RG, Johns CW, Muir PS (1980) Genetics and cytology of thems 3 male-sterile soybean. J Hered 71:343–348
Palmer RG, Kaul MLH (1983) Genetics, cytology and linkage studies of a desynaptic mutant in soybean. J Hered 78: 260–264
Rubaihayo PR, Gumisiriza G (1978) The causes of genetic male-sterility in 3 soybean lines. Theor Appl Genet 53:257–260
Sadanaga K, Newhouse K (1982) Identifying translocations in soybeans. Soybean Genet Newslett 9:129–130
Shorter R, Byth DE (1975) Multiple seedling development in soybeans. J Hered 66:323–326
Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. Iowa State Univ Press, Ames Iowa
Sorrells ME, Bingham ET (1979) Reproductive behavior of soybean haploids carrying thems 1 allele. Can J Genet Cytol 21:448–455
Stelly DM, Palmer RG (1980a) A partially male-sterile mutant line of soybean,Glycine max (L.) Merr.: Inheritance. Euphytica 29:285–303
Stelly DM, Palmer RG (1980b) A partially male-sterile mutant line of soybean,Glycine max (L.) Merr.: Characterization of themsp phenotypic variation. Euphytica 29:539–546
Yee CC, Jian L (1983) Allelism tests of Shennong male-sterile soybean L-78-387. 2nd Assembly Symp Genet Soc China, Sec 4, No 053, pp 241–242 (in Chinese)
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Communicated by A. L. Kahler
Joint contribution: Agricultural Research Service, US Department of Agriculture, and Journal Paper No. J-11255 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA 50011, USA. Project 2471
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Oliver Chen, LF., Heer, H.E. & Palmer, R.G. The frequency of polyembryonic seedlings and polyploids from ms1 soybean. Theoret. Appl. Genetics 69, 271–277 (1985). https://doi.org/10.1007/BF00662441
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DOI: https://doi.org/10.1007/BF00662441