Breast Cancer Research and Treatment

, Volume 25, Issue 1, pp 29–35 | Cite as

Comparative effect of embryonic mouse fibroblasts (Balb/c-3T3) on the proliferation of hormone-dependent (T-47D) and hormone-independent (MDA-MB-231) human breast cancer cell lines

  • Gerard Chetrite
  • Laurence Delalonde
  • Jorge R. Pasqualini


The effects of cellular extracts (CE) and conditioned medium (CM) of embryonic mouse BALB/c-3T3 (clone A 31) cells on the proliferation and DNA content of hormone-dependent T-47D and hormone-independent MDA-MB-231 breast cancer cell lines were explored. After 6 days of culture, CE and CM provoke an intense proliferative effect in T-47D cells which correlates with DNA content. In contrast, in the MDA-MB-231 cells a significant inhibitory effect was observed. For both CE and CM the action was dose-dependent. In the T-47D cells, the CM can abolish the inhibitory effect provoked by the potent antiestrogen ICI 164,384. It is concluded that mouse embryonic BALB/c-3T3 cells contain factors which can stimulate or inhibit the growth of human mammary cancer cells.

Key words

growth factors antiestrogens embryonic cells breast cancer cells progesterone receptor 


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Gerard Chetrite
    • 1
  • Laurence Delalonde
    • 1
  • Jorge R. Pasqualini
    • 1
  1. 1.Foundation for Hormone ResearchC.N.R.S. Steroid Hormone Research UnitParisFrance

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