, Volume 31, Issue 2, pp 73–78 | Cite as

1.3E2, a variant of the B lymphoma 70Z/3, defective in activation of NF-κB and OTF-2

  • John W. Rooney
  • David W. Emery
  • Carol Hopkins Sibley


The mouse B-cell cell lymphoma 70Z/3 is a convenient model system in which to study the regulation of immunoglobulin synthesis. Three transcriptional activators of kappa (κ) light chain synthesis have been identified for these cells: bacterial lipopolysaccharide (LPS), interferon-γ (IFN), and interleukin-1 (IL-1). The response of the κ gene in 70Z/3 cells to LPS is mediated by increases in two transcription factors: NF-κB and OTF-2. In contrast, IFN has no effect on either of these factors in 70Z/3 cells. We have isolated by immunoselection an LPS IFN+ variant of 70Z/3 called 1.3E2. We show here that LPS treatment of these cells causes no increase in nuclear localization of either NF-κB or OTF-2. Although they have normal levels of cytoplasmic NF-κB, it cannot be activated by LPS or by phorbol 12-myristate 13-acetate (PMA) treatment of the cells. These experiments expand the genetic dissection of the molecular pathways of activation of κ transcription in 70Z/3 cells.


Lymphoma Transcriptional Activator Light Chain Cell Lymphoma Nuclear Localization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • John W. Rooney
    • 1
  • David W. Emery
    • 1
  • Carol Hopkins Sibley
    • 1
  1. 1.Department of Genetics (SK-50)University of WashingtonSeattleUSA

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