Immunogenetics

, Volume 31, Issue 2, pp 73–78 | Cite as

1.3E2, a variant of the B lymphoma 70Z/3, defective in activation of NF-κB and OTF-2

  • John W. Rooney
  • David W. Emery
  • Carol Hopkins Sibley
Article

Abstract

The mouse B-cell cell lymphoma 70Z/3 is a convenient model system in which to study the regulation of immunoglobulin synthesis. Three transcriptional activators of kappa (κ) light chain synthesis have been identified for these cells: bacterial lipopolysaccharide (LPS), interferon-γ (IFN), and interleukin-1 (IL-1). The response of the κ gene in 70Z/3 cells to LPS is mediated by increases in two transcription factors: NF-κB and OTF-2. In contrast, IFN has no effect on either of these factors in 70Z/3 cells. We have isolated by immunoselection an LPS IFN+ variant of 70Z/3 called 1.3E2. We show here that LPS treatment of these cells causes no increase in nuclear localization of either NF-κB or OTF-2. Although they have normal levels of cytoplasmic NF-κB, it cannot be activated by LPS or by phorbol 12-myristate 13-acetate (PMA) treatment of the cells. These experiments expand the genetic dissection of the molecular pathways of activation of κ transcription in 70Z/3 cells.

Keywords

Lymphoma Transcriptional Activator Light Chain Cell Lymphoma Nuclear Localization 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • John W. Rooney
    • 1
  • David W. Emery
    • 1
  • Carol Hopkins Sibley
    • 1
  1. 1.Department of Genetics (SK-50)University of WashingtonSeattleUSA

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